...the ins and outs of this classification system, including its history, biology, and usages in the lab. History...peptidoglycan membrane. Common gram positive bacteria include staphylococci and streptococci. Gram negative bacteria... lipid membrane. Common gram negative bacteria include salmonella and E. coli. Because of their thicker... For more specific procedural instructions, including timing, preparing the smear, and mixing the stains...

...ways foreign DNA can be introduced into cells including transformation, transduction, conjugation, and...often comes from adjacent lysed bacteria and can include plasmid DNA or fragmented DNA released into the...Addgene. For more information on viral vectors, including transduction download our Viral Vectors 101 eBook...into a eukaryotic cell through non-viral methods including both chemical and physical methods in the lab....

...GCE, each component of the experiment system (including media, cell type, and growth temperature) may ...to use a protocol that you’ll have to rework to include reagents accessible to your lab. The solution GCE...is for recombinant expression of phosphoserine-including proteins in E. coli (Zhu et al., 2022). It uses... While they’ve shortlisted several protocols, including their phosphoserine ncAA protocol, to optimize...

...example, Addgene’s contributions by Tyler Ford included reagent sharing and were presented at the ASPB...at this specific conference, and I was able to include and exclude different modules for this workshop...learned: Since early 2018 the team has grown to include experts who could help provide new materials and...assembled the right people at the right time. Be inclusive and seek solutions by working with communities...

...also be designed with other DNA-binding domains, including the commonly used LexA and rTetR systems such ...could be delivered throughout the nervous system, including the motor cortex, cerebellum, and spinal cord....easily adaptable to neuroscience applications, including optogenetics, and they should make it much easier...Other proteins may also be utliized as scaffolds, including the set of red fluorescent proteins. The T-DOGGs...

... delivery technologies have been investigated including lentiviruses, adenovirus, and inorganic delivery...additional safety benefits: they lack all viral genes, including those that are responsible for integration into...capable of infecting a wide range of cell types including but not limited to muscle, liver, and brain cells...clinical trials have been directed at diseases including but not limited to hemophilia B, LPL deficiency...

.... This tool-kit includes an array of FPs as well as additional useful tags, including 3xFLAG epitopes,... chromatin. A fluorescent protein can still be included for visualization purposes, but the key component...green. This system can be used in many cell types including endothelial cell-pericyte, neuron-astrocyte, and...

...contains both fragments of split mVenus (aa 155), includes I152L in N-terminal fragment An enhanced recombinant... Dan Mulvihill mVenus Split mVenus (aa 155), includes I152L in N-terminal fragment Constant rate of ...  When designing experiments, it’s important to include proper controls to assess the behavior of the split...Biosensors Collection Split Luciferase systems, including NanoLuc®, DULIP, LuTHy Other split protein tools...

...for affecting change. On Friday, the symposium includes a panel discussion and keynote by former congressman...implementation and compliance for the FLSA changes, including finding money in already tight lab budgets and...their advocacy skills. Friday’s symposium will include a discussion on effective advocacy strategies on... on various levels, with panelists including: Kate Stoll, PhD - Senior Policy Advisor MIT Washington ...

...unstained flow controls). Spectral flow cytometers include a tool called “autofluorescence extraction” which...various tissue types varies, it is critical to include an unstained sample for each tissue type you are... the expression levels of the markers you are including in your panel. Highly expressed markers should...during unmixing. Additionally, it is critical to include unstained beads or cells within your reference ...

...Over the years, Addgene’s repository has grown to include many CRISPR tools.  Distributing and depositing...this tell us? Well, it’s hard to draw any exact conclusions, but it certainly suggests that Addgene depositors...approximately 104 plasmids shipped per working day, including appropriate MTAs and customs forms. Of course,...repository have been cited a total of 23,006 times, including 14,677 unique citations.  CRISPR is a story that...

...are required for any application. If you want to include a homology directed repair (HDR) template to create... table below summarizes the various components included with each expression system as well as suitable...components into mammalian cell lines is quite broad and includes several different methods, so we've summarized...Difficulty Hard Hard Hard These tables are not inclusive of all methods, nor are these methods limited ...

...addressed in a somewhat cursory manner, their inclusion here adds much needed context to understand every...to see the dirty laundry exposed here, but it’s unclear how much they will actually care. The section of...state of science would be complete without the inclusion of some interesting case studies, and Zimmer provides...who originally isolated the GFP gene but was not included amongst the Nobel prize winners for developing...

...beer. Ha! We had over 60 people join the fun - including a lot of future Addgenies as shown in the photo...employees. We now have a team of three (myself included) that plan big company events and fun things to...people you spend 40+ hours with each week. In conclusion - get out there any have some fun together! Laura...at all really! We've had plenty of events that included a simple corn hole set and a cooler. Chances are...

... flexibility is their broad range of targets, including proteins, peptides, amino acids, drugs, metal ...biosensors are used in a variety of applications, including detection of environmental pollutants, drug discovery...aptamers for use in many entirely new applications including aptamer based fluorescent biosensors. Aptabeacons...-based instead of protein-based fluorophores, including: Environments with higher temperatures, since...

...you start reading, there will be a temptation to include every piece of information that was ever published...figures/schematics or tables. And don’t forget to include citations so that people can go back and read the...studies that reported mutations in that protein (including how frequently a mutation was found and the size...doesn’t have to be revolutionary, but you could include a few comments on where you think the field is ...

...administered, which leads to many complications, including the inability of some institutions to know exactly...Furthermore, 7% did not enforce these policies. It is unclear how many postdocs are at each of these institutions...are afoot for the postdoc population, and it is unclear how this will all shake out. In the short term,...would help our resource. In the medium term, it is unclear what effects the new laws will have on the postdoc...

...that went beyond obtaining “omic” data and had to include clear plans to connect Genotype to Phenotype thus... is to ensure that, in general, these labs all include materials sharing in their dissemination plans ... diversity in the social bonds that they form including genetic mechanisms that control who a female wants...of basic evolution of fungi and eukaryotes and includes the fungus that is killing frogs all over the ...

...completely understood, but some possible reasons include: Metabolic pressures - it takes cellular resources...when designing a full gene. A non-exhaustive list includes: Codon abundance relative to cognate tRNA abundance...insoluble, nonfunctional globs of protein known as inclusion bodies that will generally segregate with the ...assay to make sure your protein isn’t forming inclusion bodies and is folding properly. References 1...

...domain - VP64.  Later generations of activators include the SunTag system which facilitates recruitment...Addgene’s website. Fig. 3: Cas9 repressors include dCas9 alone and a dCas9-KRAB fusion, both targeted...targeted to the promoter region. Cas9 activators include Cas9 fused to VP64, VP64 along with other SAM factors...expression in different species and cell types including (but not limited to) bacteria, yeast, plants, ...