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We narrowed to 440 results for: pes;

Showing: 81 - 100 of 440 results

  1. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    Published
    Nov. 24, 2015, 3:30 p.m.
    ...to express a variety of proteins in single cell types in the mouse, but knew it would take years to develop...established mouse lines expressing GFP in specific cell types to do more than just label cells. If GFP could be...see if they could apply GFP regulation to other types of proteins. They started with another biological...their scaffold system is likely applicable to many types of proteins. For proteins with a modular structure...

  2. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ... also capable of infecting a wide range of cell types including but not limited to muscle, liver, and ...brain cells. There are a multitude of known AAV serotypes that have slight variations in their viral capsids...over many years in postmitotic, long-lived cell types. AAVs in the clinic Thus far, AAV vectors AAV1,...prevalence of neutralizing antibodies against some AAV types, AAV1, 2, 3 and 5 are thought to be as high as 70%...

  4. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ...same holds true for types of viruses generated in the lab. The four most common types of viruses grown in...involve needles or scalpels (sharps). Cuts and scrapes from sharp equipment are one of the most common...Resources on the Addgene blog Viral Vectors 101: Types of Viruses Viral Vector Elements Tips for Preventing...

  5. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...from cells or using live cells. There are several types of expression systems used for protein production...post-translational modifications. There are two types of insect expression systems; baculovirus infected...integrated into the insect cell genome. Both of these types of insect expression systems can be scaled up for...of recombinant proteins. Many cells from various types of plants such as maize, tobacco, rice, sugarcane...

  6. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...Why Expansion Microscopy?  Traditional light microscopes are limited in the level of resolution that they...you might expect them to dramatically limit the types of labels you could use. But many ExM protocols ...reagents, straightforward protocols, and standard microscopes sounds great, right? But there is always a catch...loss of protein during digestion, or masking of epitopes during sample anchoring and gel polymerization...

  7. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    Published
    July 25, 2024, 1:15 p.m.
    ...proteins and small molecules from a variety of sample types (Engvall 1971). Scientists value ELISAs for their...Herein, we’ll give you a rundown of the different types of ELISA, advantages and disadvantages of each, ...detection methods.      Figure 1: The four types of ELISA are direct, indirect, sandwich and competitive...antibody must bind to distinct, non-overlapping epitopes so that they do not interfere with each other....

  8. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...instead of fluorescent markers. There are various types of SSC and FSC that can be used for this purpose...). This gating strategy is used to identify two types of dendritic cells (DCs), mDCs and pDCs. Let's follow...Cells are gated, and in the gated population, two types of FSC are used to separate single cells from doublets...under Creative Commons license.    Pro tip! Subtypes of immune cells are often defined by a subset ...

  9. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ... that the late replication that occurs for some types low copy plasmids during cell division simply does...microbiological world. (Want to know more about these plasmid types? Read our Plasmids 101: Environmental Plasmids blog... plasmids from the bacterial pathogens Yersinia pestis, Agrobacterium tumefaciens, and Bacillus anthracis...) Curing of four different plasmids in Yersinia pestis using plasmid incompatibility. Letters in Applied...

  10. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...Differences in the physiology of different cell types, however, can make this assumption incorrect. One...lentiviral vectors are engineered with non-native envelopes, a process called pseudotyping. Lentiviruses pseudotyped... cell type may not translate well to other cell types. To overcome this issue, try transducing with a ...PMC1368960. 10. Denning, W., Das, S., Guo, S., Xu, J., Kappes, J.C., Hel, Z. “Optimization of the transductional...

  11. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...size. Like CRISPR, there is a variety of small RNA types to choose from and can be catered for your specific...of different applications that involve different types of small RNA molecules, such as: Micro-RNA (miRNA...and piRNA can be found endogenously in many cell types, while shRNA and siRNA are synthetic constructs ... end_module_attribute %}{% module_attribute "field_types" is_json="true" %}{% raw %}{"button_text":"text...

  12. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...antibody-peptide labeling; specifically, cognate peptide epitopes fused to the protein of interest are recognized...system, dCas9 is fused to a scaffold containing epitopes recognized by scFV antibodies fused to VP64 transcriptional...for inducible expression, loxP sites, H2B, P2A or PEST2D sequences. Several pre-constructed promoter-selection...AUUUA [5 consecutive]) and protein (C-terminal CL1 & PEST). These modifications ensure that any signal from...antibodies. Unfortunately, while there are many types of epitope tags (eg. HA, MYC, Flag), they can be...them to GFP, and compared them to popularly used epitopes tags such as HA and MYC in vivo. The authors found... between the POI and a TAP tag (a tag with two epitopes that can be used to sequentially purify the POI...

  13. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...brain cell types. Look for more of these interneuron-targeting constructs and other serotypes available...Lacy Targeting gene expression to specific cell types in the brain can be a challenging — but critical... to target and manipulate distinct interneuron subtypes. Neuron, 113(10), 1525–1547.e15. doi: https://...

  14. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...BioRender.com.   Optogenetic tools Types of control We will discuss three types of tools: light-gated ion channels... been developed to selectively study individual types of GPCRs with greater spatial and temporal control...’ll need to know (and understand!) the hardware types, construction, and testing used for delivering your...

  15. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...proteomic data from several different tissues or cell types. If your antibody indeed binds to your target then... the manipulation. Additionally, not all sample types are amenable to genetic manipulation, nor can all...the results. Using antibodies against different epitopes on the same target reduces the likelihood that...antibodies that are predicted to bind different epitopes of this protein.   You perform flow cytometry...

  16. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    Published
    Oct. 27, 2020, 1:15 p.m.
    ...dicer-like enzymes (DCL) process the dsRNA into specific types of interfering RNAs.   RNAi vs. CRISPR: Complementary...easily identified and have readily observable phenotypes quantified. Fluorescent reporter-based RNAi constructs... RNAi plants. With a visual reporter of RNAi, phenotypes, such as morphological parameters, can be easily... for Rapid Identification of Loss of Function Phenotypes. Plant Biology 7:251–257 . https://doi.org/10.1055...

  17. Plasmid Grant to Aid Cervical Cancer Screening in Ghana

    Type
    Blog Post
    Published
    Nov. 8, 2016, 3:30 p.m.
    ...cancers are caused by persistent infection of HR HPV types, predominantly HPV 16 and 18.” HPV plasmids as positive...genes as positive controls in her PCR screens. She hopes that these screens will both identify individuals...also better correlate the presence of certain HPV subtypes with risk and disease severity. Individuals who...

  18. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    Published
    Aug. 28, 2018, 12:04 p.m.
    ...had taken out a few foldscopes and held an improv workshop. These paper microscopes work as conventional...conventional microscopes and give a similar optical quality (magnification of 140X and 2 micron resolution). I ...

  19. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ... are suitable for your cell lines and/or tissue types.  Please do note my oh-so-subtle use of reference... on plate space. For many cell lines and tissue types, appropriate reference genes (and supporting data...unwanted variation into your data. Try different types of pipettes and pipetting methods to find what’s... it up for larger or frequently analyzed sample types and sets.   🔥Hot Tip🔥 The ABI machines automatically...

  20. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

    Type
    Blog Post
    Published
    June 11, 2019, 1:16 p.m.
    ...which let you lock and hide individual vectors (shapes defined by 2D points connected by lines and curves...wide selection of tools for drawing and editing shapes and like Vectr, Inkscape uses vector graphics. ...settings let you tailor your search to particular types of images. For example, by selecting “Tools” and...
Showing: 81 - 100 of 440 results