Skip to main content

We narrowed to 84 results for: plasmid cloning by pcr

Showing: 81 - 84 of 84 results
  1. Hot Plasmids: May 2026

    Type
    Blog Post
    Published
    May 5, 2026, 1:15 p.m.
    ...research, popular plasmids for a common lab enzyme, and more! Read on to find: Plasmids and recipes for...Every few months we highlight some of the new plasmids, antibodies, viral preps, and other materials ...materials in the repository through our Hot Plasmids articles. This month, we’ve got some new biosensors for neuroscience...Fortunately, the Pojer and Arrowsmith Labs have shared plasmids you can use to express and purify your own supply...your preference or needs. Plus, the MBP-Benzonase plasmid is available to industry as well as academic scientists...(Figure 1). Nearly 100 labs have ordered these plasmids already. Join them and turbocharge your own preps...preps today! Find SerMNucA Benzonase plasmids here! Rieubon, L., Lim, M., Lau, K., Pojer, F. (2026). Purification...
  2. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...They generated plasmids tagged with EB3 to mark the growing tips of microtubuli and plasmids tagged with ...and dCas9-VPR targeting plasmids can be found at Addgene. Check out these plasmids for piggyBac gRNA delivery...highlights from recent CRISPR plasmids. To find all of the CRISPR plasmids available from Addgene, head... Find the plasmids at Addgene The MoClo CRISPR/Cas Toolkit for plants includes 95 plasmids consisting...need. If you'd ever like to write about a recent plasmid deposit please sign up here.  Here's what you'll...
  3. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    Published
    July 13, 2023, 1:15 p.m.
    ...single plasmid transformation, though, a pooled library has hundreds to millions of distinct plasmids, all...anyone who has worked with individual plasmids knows, some plasmids amplify well and others don’t. The size...pooled library, which contains a collection of plasmids in a single tube, or a pre-made viral prep library... prepare the libraries for you.  Starting from plasmids requires amplification, verification, and lentiviral...library and appropriate packaging and envelope plasmids into HEK cells allows production of lentivirus...amplify the library. This is similar to amplifying a plasmid: transform E. coli with the pooled library DNA,...own protocol that amplifies without disrupting plasmid representation. A good place to start is with a...
  4. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    Published
    March 21, 2024, 1:15 p.m.
    ...self-labeling protein fusions as well as empty backbones to clone your own protein of interest. Fluorescence microscopy...cell and protein analysis products. You can find plasmids deposited by Promega here.    References and ...Self-Labeling Protein Tags Fluorescent protein plasmids and resources A practical approach to choosing...key, especially for G-protein-coupled receptors (GPCRs). Mutations in receptor sites can affect the fluorescence...
Showing: 81 - 84 of 84 results