...about Popular Plasmids in the Repository Learn about New Plasmid Technologies in our Hot Plasmid Articles ...to create a plasmid based on the biotin ligase BirA backbone (Eric Campeau, Addgene plasmid #26624) was...inserting BirA into a pMal plasmid (NEB) thus creating the pMal-T-Avi-His/BirA plasmid now available through...other Hot Plasmids Find Additional Posts on Mol Bio Techniques Find Additional Posts on Plasmid Technologies...controlled easier than formation of GST dimers. Avi-Tag plasmid for improved biotinylation selectivity A common...exploit the best of both worlds. We generated a plasmid that produces biotin ligase and a protein of interest...and a 6X HisTag for affinity purification (see plasmid map and cartoon below). Including biotin in your...

...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...about a recent plasmid deposit please sign up here.   Listen this episode of Hot Plasmids! Light controls...Hahn's lab at the University of North Carolina (plasmids at Addgene). It consists of two domains, LOV2 ... the same number of fluorophores. You can find plasmids containing the cobalamin binding riboswitches ...the Reuben Harris lab has deposited a reporter plasmid that allows for rapid fluorescence-based quantification...editing complexes are more efficient than the BE3 plasmid containing rat APOBEC1. Finally, they show that...

...resource: Recombinant Antibodies! In addition to plasmids and viral vectors, you’ll now be able to order...et al., 2015). All of Addgene’s antibodies are plasmid-based and recombinant. Learn more about recombinant...infrastructure, from our educational materials to our repository of open samples. We are, and have always been...other recombinant affinity reagents, share your plasmids via Addgene! Email [email protected] to learn...support, we have enabled scientists to easily share plasmids with each other. Together, we can transform the...

...Resources & Plasmids Plan Your CRISPR Experiment Synthetic Biology Resources & Plasmids  ...addition to the poster session was a first for our repository, and I was proud to be responsible for sharing...poster were impressed to see how the numbers of plasmid genome engineering tools – both deposited and distributed...expressed gratitude for the role Addgene plays in plasmid sharing to those who were anxious to hear about...

...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...need. If you'd ever like to write about a recent plasmid deposit please sign up here.        New toolkit... the Kanemaki lab has created a new toolkit of plasmids for the N- or C- terminal tagging of proteins ...previously difficult. Find SpCas9 and Target-AID-NG plasmids at Addgene! Nishimasu H et al., Science, 2018....datasets for research and innovation. Find MerMAIDs plasmids at Addgene Opperman J et al., Nature Communications...inducible and reversible nature. Find the LADL plasmids at Addgene Kim et al., Nat Methods. 2019, PubMed...

...preference to journal access granted via a public repository or authors’ own web sites. 9)      The year 2013...Share Science, in honor of a decade spent making plasmids more accessible and easier to share. As we prepare...

...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...need. If you'd ever like to write about a recent plasmid deposit please sign up here.  Human whole genome...attached fluorescent protein to the DNA. They created plasmids for purification of tTALE-eGFP and tTALE-mCherry...digestion of single molecules in real-time. Find plasmids expressing tTALE-FP Shin et al., Sci. Rep. 2019...

...SnapGene powered plasmid maps at Addgene  Three factors make our SnapGene powered plasmid maps far easier...easier for you to share plasmid information with colleagues, or explain plasmid features to a lab member... on the main plasmid page under the "Resource Information" section. Analyze your plasmid sequence For ...it much easier to browse the repository and quickly identify the plasmids and features you need for your...over from our users: "Please, please improve your plasmid maps!" After thoughtful design, vetting, and tweaking...tweaking, we’re excited to announce that our plasmid and sequence displays are now powered by GSL Biotech's...software and extensive feature library, our updated plasmid and sequence displays are now much easier to interpret...

...of the new plasmids, antibodies, and viral preps in the repository through our hot plasmids articles. ... missed something smokin'? You can pitch a Hot Plasmids section (antibodies and viral preps welcome!) ...Figure 3 Colognori et al 2022.   Find CRISPR Csm plasmids here! Colognori D, Trinidad M, Doudna JA. Precise...inducible expression.   Find heat-inducible lentiviral plasmids here!   Wu Y, et. al. Control of the activity ...2: Addgene’s western blot for mEGFP-HRas (from Plasmid 18662). See the Applications section on the Anti-GFP... Campbell et al, Nature Methods   Find YFP plasmids here!   Campbell, B.C., et al. Chemically stable...

...toolbox. Natural plasmids, also called environmental plasmids, are all around us! A plasmid is a small piece...colicin-producing plasmids have been referred to as “Col plasmids” or “Col factors.” Virulence plasmids: microbial...Old Solution but a New Problem Plasmids 101: What is a Plasmid? Plasmids 101: Transformation, Transduction...F. (2023). Plasmid classifications. Plasmid, 126, 102684. https://doi.org/10.1016/j.plasmid.2023.102684...., 2001) Beneficial traits of natural plasmids Natural plasmids often convey a variety of functions that...functions of natural plasmids. Created with BioRender.com. Resistance (R) plasmids: microbial defense...example of a plasmid benefiting its host. For example, cells with a penicillin resistance plasmid can grow...

...sharing plasmids through Addgene is that we provide an added level of confidence in the plasmids we distribute...ensuring plasmid quality is to stay organized. Here at Addgene, we collect data on every plasmid we distribute...cloned into the plasmid) and any other important features that differentiate this plasmid from its predecessor...sequence analysis, plasmid verification, and using NCBI BLAST. Tips for high-throughput plasmid sequencing If...on the Addgene Website View our Plasmid Reference Pages Get Plasmid Backbone Information from Our Vector...distribute through our quality control processes. Every plasmid we receive is rigorously verified before becoming...community. This is no small task, however, at a repository that consistently receives around 200 new DNA...

...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...need. If you'd ever like to write about a recent plasmid deposit please sign up here.        A retroviral...treatment of cardiovascular disease. Find the LipoGlo plasmids at Addgene! Lawler et al, QJM: An International...promoter systems. Find these CRISPR activation plasmids at Addgene. Liu et al., Nature Communications,...

...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need... recent plasmid deposit please sign up here.          Listen to this episode of Hot Plasmids! Optogenetic...Grefen and Blatt, 2012). The 2in1 Plasmid Toolkit consists of 4 plasmid toolkits generated with 4 different...deposited the resulting optogenetic plasmid tools which includes plasmids encoding a fusion between cPYP and...targeting and editing experiments. Find the CASANOVA plasmids here! Bubeck F et al., Nature Methods 2018. PubMed...as mice, C.elegans, and Zebrafish. Find Archon1 plasmids at Addgene! Piatkevich KD et al, Nat Chem Biol...

...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...need. If you'd ever like to write about a recent plasmid deposit please sign up here. Here's what you'...easily be applied to other systems.    Find BLITZ Plasmids here! Xiong, et al. eLife. 2021. https://doi.org...from Goertsen D, et al., 2022.  Find M-CREATE plasmids here!  Goertsen D, et al. Nature Neuroscience....have higher risks of off-target mutations. These plasmids contain catalytically inactive dCas9 and microbial...Image from Wang, et al, 2022.  Find dCas9-SSAP plasmids here! Wang et al. Nat Cell Biol. 2022. https:/...

...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...need. If you'd ever like to write about a recent plasmid deposit please sign up here.  Here's what you'...Ladder  by: Rachel Leeson This simple, inexpensive plasmid-based ladder system is a cost-effective alternative... ladders. Image from Santilli, et al. 2021 Plasmids are used to express 10, 15, 20, 30, 40, 50, 60...kD proteins or 20, 40, 60, and 80 kD proteins. Plasmids containing 150 or 250 kD proteins are also available...

...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...write about a recent plasmid deposit please sign up here.  Listen to the hot plasmids episode! New, highly...hundred SH3 domain containing plasmids available through Addgene. These plasmids can be used to carry out ...dsRNA necessary for RNAi. The Vellai lab’s new plasmid, T444T, contains T7 terminators adjacent to the...to increased RNAi efficiency. Find the T444T plasmid here! Strum A, et al. Nucleic Acids Research. 2018... Drosophila and C. elegans.   Find the TurboID plasmids here! Branon et al. Nat Biotechnol. 2018 PubMed...

...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need... recent plasmid deposit please sign up here.      Listen to this episode of the Hot Plasmids podcast ... (ARG) plasmids to better monitor gene expression in vivo using ultrasound. These ARG plasmids contain...responses in C. elegans. Find the split BoTN/B plasmids at Addgene. Liu Q et al., Neuron. 2019. PubMed...potentially problematic for imaging. Find the ARG plasmids at Addgene. For more details on acoustic reporters...s collection consists of a library of reporter plasmids, each containing two fluorescent proteins and ...

...necessarily mean the repository does not have it. That said, if you know of a plasmid that we definitely...Chromatin-related genes with plasmids available from Addgene The links below pull up lists of plasmids containing the...containing lists of plasmids from the indicated publications. You can request all of these plasmids directly from...consolidate every conceivable chromatin-related plasmid in a collection that grows on a daily basis, the...study chromatin and want to start sharing your plasmids through Addgene, don’t hesitate to start the process...these pages to learn when Addgene receives new plasmids containing these genes. As with the PIs above,... In-exhaustive Chromatin-related genes with plasmids on Addgene.org AGO1 BRCA1 CREBBP CUL1 H2AFX...

... If there’s a plasmid or AAV tool that you can’t find, you can always suggest a plasmid to us, request...want to get into the field. Find Optogenetics Plasmids for Use in Neuroscience Research Neuroscience ...400 who signed up for our newsletter and a free plasmid) were very excited about our new viral services... efficiently. For select viral vectors in the repository, you can instead have Addgene make the virus ...Service Find Retrograde AAV Browse Optogenetics Plasmids ...

...Resources on Addgene.org Browse Cancer Plasmids Browse Immunology Plasmids Find Kits for Cancer Research  ...immunosurveillance have a look at our Immunology Research Plasmids and Resources. Studying the immune system and ...potential to become an open-source pipeline and data repository for cancer researchers. Go open-source! Impressions...analyse the tumor microenvironment (one of the plasmids on her poster is pTomo-Ras which has been deposited... immunology resources. If you have a great new plasmid you’d like to share with the scientific community...