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We narrowed to 188 results for: pme i

Showing: 81 - 100 of 188 results

  1. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    .... However, I have a list of what I really need in order to reach my research goals. Would I be able to...include: equipment, reagents, animals, glassware, and even people. When developing a budget, I suggest ...of the major equipment that you use in your current lab first. Generally, major equipment can be defined...requires specialized equipment, try finding institutes that offer shared equipment that you can use at ...defined as equipment with capital costs greater than $5,000 but this can depend on the institution/funding...funding source. Major equipment includes large centrifuges, sequencers and analyzers, and any ultra sensitive...sensitive equipment (RT-PCR, Microscopy, Fluorescence Scopes, Flowcytometers, etc.). After identifying all...

  2. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...thing. Nevertheless, the models are compelling, and I encourage you to hold one. Why do we need physical...physical model. Until then, you can only wonder what I am talking about. How do researchers use physical ...they will almost invariably say something like “Oh,…I didn’t quite appreciate that….” For example, when ...polypeptide emerges from the exit channel.   He then said “I hadn’t realized how perfectly flat the complex is ...an initial solution to this problem with the development of Byron’s Bender – a mechanical device that ...molecular structures for use in professional development programs for bioscience educators. Using these...

  3. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    Published
    Oct. 29, 2019, 2:52 p.m.
    ...ion-channels and pumps in developmental biology, regeneration studies, and cancer biology. I was first introduced...introduced to bioelectricity when I attended the Developmental Bioelectricity satellite symposium at the 2019...meeting I had an increased appreciation for the role of ion channels and pumps in developmental biology...2019 Society for Developmental Biology meeting in Boston. The atmosphere was electric! After attending...whole scientific community. Over the years, developmental biologists and cancer researchers are beginning... decision-making in somatic cell networks. “Developmental bioelectricity is an extremely exciting emerging... "The body electric 2.0: recent advances in developmental bioelectricity for regenerative and synthetic...

  4. Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Feb. 25, 2025, 2:15 p.m.
    ...need to push the boundaries of discovery. As I look ahead, I am excited to continue to grow and innovate...Houston, D. W., Polinski, N. K., Meijers, R., Levey, A. I., Saper, C. B., Errington, T. M., Turn, R. E., Bandrowski...science. Want to learn more about our latest developments? Subscribe to the Addgene newsletter. References...

  5. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    Published
    Oct. 7, 2014, 3 p.m.
    ...  It was with a sense of relief that I attended the amazing, postdoc-driven Future of Research Conference...supporters from all of the research centers in Boston.  I was relieved because it finally feels to me like the...the Workforce, Metrics and Incentives and Funding.  I can’t wait to see what solutions these talented postdocs...progress reports information on the Individual Development Plans (IDPs) for the graduate students and postdocs...

  6. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

    Type
    Blog Post
    Published
    Aug. 13, 2014, 3:49 p.m.
    ... of tumor antigen. I asked Howarth to tell us more about SpyLigase, its development, and its potential... different approaches to improve CTC capture, but I hope that our antibody tentacles may have an impact...plasmids that may well be of use to others to Addgene. I am a big fan; thanks for the T-shirt! References:...

  7. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    Published
    May 18, 2018, 7:32 p.m.
    ... this event, I gave my own talk on reproducibility at Addgene and here I summarize what I learned both...irreproducibility with some solutions based on what I’ve learned. I hope you can apply some of these solutions to your...align better with an initial hypothesis. To be clear, I don’t think that the majority of researchers do these...whether the ultimate results were positive or negative. I can feel you rolling your eyes, but even if this ideal...plasmid repository 49:45 0 1:18:45 - Reagent Development - Steven C. Almo, Institute for Protein Innovation...

  8. Finding and Joining Your Dream Lab

    Type
    Blog Post
    Published
    April 8, 2014, 2:50 p.m.
    ...this PI looking for in a new researcher?,” “How do I convince the PI to choose me?” We asked three leading...how it will be before you take the position. Am I choosing the right mentor? Make sure your advisors...neighbors during the interview process. What if I want a non-academic career path?  If you are considering...source software, hardware, wetware, technology development, etc). Second, the potential hire visits the ...identifying a lab that fits both work style and development needs. These two factors will provide a solid...

  9. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    Published
    July 6, 2017, 1:59 p.m.
    ...defeat it we need to attack it from all fronts!”  Here I aim to compile a few highlights from the conference...Methods to study autophagy can be found here. Fun fact: I also learned that Professor Kroemer often functions...from the poster sessions During the poster session I got a chance to talk with Dr. Friedmann-Morvinski ...CAR T cells and PD-1 modulators. An exciting development in immunotherapy is the combination of the CRISPR...PMID: 28225754. 6. Marumoto, Tomotoshi, et al. "Development of a novel mouse glioma model using lentiviral...

  10. Bringing Sustainable Practices to the Lab: Easy Action Items

    Type
    Blog Post
    Published
    April 22, 2019, 12:26 p.m.
    ...room and don’t have to keep buying more freezers. I am pretty sure that the world will go on without my...the same. Long-term lab conservation goals Lastly, I think it’s a good balance to have both short-term ...reduce your consumption in the lab: Turn off your equipment at the end of the day and when not in use. You...making dinner at night, so why leave your lab equipment on? This applies to water baths, computers, and...

  11. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    Published
    Dec. 9, 2014, 2:22 p.m.
    ...the common mode of operating.  Managing scientists I should add a few special words about managing scientists...Biotechnology article “Managing knowledge-intensive workers”. I have added a few comments of my own to this list. ...work, opportunities to pursue and problems to solve (I think this is true of most people, not just scientists...training or teaching others, research, and policy development. Every worker at every level does better with...

  12. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    Published
    Jan. 16, 2014, 2:56 p.m.
    ...Cepko to learn about her mentoring style. How can I find out if an advisor is a good mentor? You have ... more resources to help you choose a good mentor, I have provided a downloadable PDF at the end of this...scientists think, “Oh, but that won’t happen to me – I am different.”  Unfortunately, a bad mentor can be...practice successful grant writing, lab budgeting, equipment acquisition, public speaking and personnel management...

  13. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    Published
    Feb. 28, 2023, 2:15 p.m.
    ... Open source hardware builds   Since I started my PhD, I have been convinced that most microscopy ...a number of new platforms (such as openUC2, which I am a co-founder of), that aim to support the community...taking these limitations into account in the development process. Widely used, commercially available ...Squid: Simplifying Quantitative Imaging Platform Development and Deployment." bioRxiv. 2020. Diederich, B....

  14. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    Published
    Oct. 20, 2020, 1:15 p.m.
    ...   3. Start something In almost every career talk I present, I’m asked what Addgene “looks for” when hiring... The online dictionary gives two definitions that I like: “the ability to assess and initiate things independently...start? 4. Do informational interviews (on a schedule) I hope you all know by now that people get jobs at least...wait for it to be over to work on career skills development. Back in March, we posted these 25 great tips...

  15. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ...consider my first challenge when I started working with AAV vectors. I wanted to use an AAV vector to express...effort to test and optimize our vector - overall, I spent two years testing and reformulating our AAV ...from people trying to answer the exact same question I was: What AAV vector is best for my specific system...Vectors. Molecular Therapy - Methods and Clinical Development, 18(September), 98–118. https://doi.org/10.1016...

  16. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    Published
    June 23, 2022, 1:15 p.m.
    ...editing techniques is its ease of use.  “I was really excited when I saw these two papers,” said the University...conditions. Remaining hurdles in CRISPR biology “I think it is absolutely possible to use CRISPR to cure... It is speeding the process of the discovery/development of genetic targets and RNA therapies. [6]. In...

  17. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    Published
    Dec. 12, 2017, 3:13 p.m.
    ... able to edit RNA in mammalian cells with CRISPR? I certainly didn’t, but now we can with Cas13b/ADAR ...from the REPAIR (RNA Editing for Programmable A to I Replacement) system. Like RNA targeting systems, RNA... to make it easy for you to keep up with new developments (and, of course, find plasmids that will be ...CRISPR History provide a great overview of the development of CRISPR into a varied research toolbox, as ...Plasmids and Resources and CRISPR History and Development for Genome Engineering  ...

  18. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    Published
    July 2, 2019, 12:51 p.m.
    ...CRISPRa, the Day lab singled out two Bdnf isoforms (I and IV) for investigation. Co-transduction of primary...hundreds of differentially expressed genes between Bdnf I-overexpressing neurons and Bdnf IV-overexpressing ...Among the upregulated genes common between the Bdnf I and Bdnf IV overexpression variants were other immediate-early...University of Rochester Medical Center. During development, complex genetic programs specify and assemble...technological gap, a trio of recent papers describe the development of CRISPR activation (CRISPRa) tools for neuroscience...recently circumvented by the Yang Lab with the development of a Suntag-p65-HSF1 (SPH) transgenic mouse (...regulates neuronal physiology by promoting the development of neurons and synapses as well as long-term ...

  19. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...phylogenetically classified as the more complex Class I systems that contain multiple subunits, but the more...its ancestors. They classified the system as Class I, Type III-E, and then used this information to identify...03886-5. Epub 2021 Sep 6. PMID: 34489594. Eleonora I. Ioannidi, Matthew T. N. Yarnall, Cian Schmitt-Ulms...and cellular biology, this happens through the development of tools that exploit and expand our current ...which we're featuring in today's blog. Cas7-11 Development and Discovery As part of their quest to find,...Cas systems opens up the potential of further development of low-toxicity RNA editing tools.   Fig...embryonic stem cells and glioblastoma cells. PASTE Development and discovery The first generation of CRISPR ...

  20. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type
    Blog Post
    Published
    April 2, 2019, 1:44 p.m.
    ...protein after a vegetable substitute for spaghetti? I actually couldn’t find the specific origins of the...does not form properly (Azplazu and Frasch, 1993). I could not find the exact origin of this gene name ...contains bagpipe from the Deplancke lab. Conclusion I hope you enjoyed this tour of Addgene’s new conference...primarily involved in development. These genes are known to play a role in neural development, sex determination... a transcriptional co-repressor important in development. Groucho family proteins are found in flies, ...Hedgehog, an important regulator of embryonic development The largest conference room at Addgene is named...rather to an extremely important gene in animal development. To understand where sonic hedgehog got its name...
Showing: 81 - 100 of 188 results