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We narrowed to 281 results for: prep

Showing: 81 - 100 of 281 results

  1. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ...highlight a subset of the new plasmids and viral preps in the repository through our hot plasmids articles...

  3. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    Published
    Feb. 2, 2021, 2:15 p.m.
    ... program coordinator.   6. Virtual interview prep Prepare for your virtual interview just as you would...that program of your dream.  “Chance favors the prepared mind” by Louis Pasteur These tips are a set of...of common sense approaches that will prepare you for the competitive landscape beyond college. They will...guarantee of success. Don’t luck into an internship, prepare the road ahead for success. Every student should...

  4. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...adeno-associated virus (AAV). We also offer viral prep services for some of the plasmids in our collection...

  5. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...These sensors have been tested in vivo, in tissue preps, in multiple species, and in cell culture models...

  11. Common Injection Routes in Mice

    Type
    Blog Post
    Published
    Jan. 22, 2025, 9:42 p.m.
    ...events. Intradermal injections require a bit of extra prep, as the injection site area must be shaved first...

  12. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...some of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles...variant (Piatkevich et al., 2023). Check out their preprint for more details. Not only will these mStayGold...doi.org/10.1038/s41592-023-02085-6. PMID: 38036853. Preprint: https://doi.org/10.21203/rs.3.rs-2941917/v1.  ...doi.org/10.1038/s41587-023-02018-w. PMID: 38081970. Preprint: https://doi.org/10.21203/rs.3.rs-2684100/v1.  ...proteins derived from StayGold. ResearchSquare (Preprint). https://doi.org/10.21203/rs.3.rs-3188559/v1....

  14. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...chemogenetics in our chemogenetics guide Find viral preps for your research Find chemogenetics plasmids for...

  15. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...about your research goals and education, too! Prepping for the interview Many universities offer free...

  17. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ...genome-containing viral particles that are present in an AAV prep. AAV samples are first DNaseI digested to remove...still necessary. To save time and money, I like to miniprep 5 mL of my AAV transfer plasmid cultures and then...pellet for the remainder of the cultures and later maxiprep only the cultures with intact ITRs. I use a spreadsheet...culture supernatant because it’s time consuming to prepare the 40% PEG solution. PEG takes several hours on.... Rather than investing all of this effort in preparing reagents, I choose to only harvest the cell pellet...separate out contaminants from an impure AAV preparation. 15%, 25%, 40%, and 60% iodixanol solutions are...double-check that the iodixanol gradient solutions were prepared correctly. When aliquoting purified and buffer...

  18. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ... minutes. Additionally, while commercial plasmid prep kits come in discreet sizes (mini, midi, maxi, giga...purification kits. Kit-free alkaline lysis plasmid miniprep Starting material: 2 mL bacterial culture Product...Product: plasmid DNA This kit-free plasmid miniprep protocol from Addgene follows a similar workflow as ...authors estimate that a gravity-based column plasmid maxiprep kit takes 1-1.5 hours to complete, while this ....0007750  Vogelstein, B., & Gillespie, D.A. (1979). Preparative and analytical purification of DNA from agarose...

  19. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...vector preparations, we have used quantitative PCR to evaluate the physical titer of our preps. We often... for ddPCR set-up. Prepare the master mix in a separate area from where you prepare your sample dilutions...

  20. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...Addgene Browse all AAV vectors Find ready-to-use AAV preps ...STORM imaging, and electron microscopy. Sample preparation for these techniques sometimes makes it more ...retain their ability to be detected after sample preparation better than standard proteins tags like GFP. ...
Showing: 81 - 100 of 281 results