We narrowed to 286 results for: prep
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TypeBlog PostPublishedMay 26, 2022, 1:15 p.m....highlight a subset of the new plasmids and viral preps in the repository through our hot plasmids articles...
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Hot Plasmids - February 2022
TypeBlog PostPublishedFeb. 22, 2022, 2:15 p.m....highlight a subset of the new plasmids and viral preps in the repository through our hot plasmids articles... -
Antibodies 101: Buffers, Storage, and Conjugates
TypeBlog PostPublishedFeb. 1, 2022, 2:15 p.m....not just the antibodies that matter when you’re prepping for your experiment - there are a number of outside... -
7 Tips to Secure a STEAM Internship This Summer
TypeBlog PostPublishedFeb. 2, 2021, 2:15 p.m.... program coordinator. 6. Virtual interview prep Prepare for your virtual interview just as you would...that program of your dream. “Chance favors the prepared mind” by Louis Pasteur These tips are a set of...of common sense approaches that will prepare you for the competitive landscape beyond college. They will...guarantee of success. Don’t luck into an internship, prepare the road ahead for success. Every student should... -
Plasmids 101: Screening Strategies Used in Plasmid Cloning
TypeBlog PostPublishedSept. 15, 2020, 1:15 p.m....using lysed bacterial colonies, without the need to prep your plasmid. In this case, you’d be using colony... -
Popular Retroviral Vectors and Their Uses in Scientific Research
TypeBlog PostPublishedJuly 24, 2018, 12:51 p.m....to produce virus? Get the ready-to-use lentiviral prep from the Addgene viral service. CRISPR technology... -
Giving gRNAs a Facelift - Synthetic and Beyond
TypeBlog PostPublishedOct. 20, 2022, 1:15 p.m....Addgene's CRISPR resources Request ready-to-use viral preps of select CRISPR lentiviral plasmids Additional... -
GPCRs: How Do They Work and How Do We Study Them?
TypeBlog PostPublishedFeb. 11, 2021, 2:15 p.m.... on neuron activity (firing or inhibition). AAV preps from DREADD plasmids allow you to easily express... -
Supporting Rare Disease Research with the 2020 BeHEARD Awards
TypeBlog PostPublishedSept. 8, 2020, 2 p.m....research and how they will use plasmids and viral preps from Addgene to further their work. The Association... -
Synthetic promoter AAVs for cell-type specific expression in retinal cells
TypeBlog PostPublishedMarch 12, 2020, 1:15 p.m.... Find AAV plasmids Find ready-to-use AAV viral preps Browse all viral vectors References Juettner, J... -
Common Injection Routes in Mice
TypeBlog PostPublishedJan. 22, 2025, 9:42 p.m....events. Intradermal injections require a bit of extra prep, as the injection site area must be shaved first... -
Hot Plasmids February 2024
TypeBlog PostPublishedFeb. 20, 2024, 2:45 p.m....some of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles...variant (Piatkevich et al., 2023). Check out their preprint for more details. Not only will these mStayGold...doi.org/10.1038/s41592-023-02085-6. PMID: 38036853. Preprint: https://doi.org/10.21203/rs.3.rs-2941917/v1. ...doi.org/10.1038/s41587-023-02018-w. PMID: 38081970. Preprint: https://doi.org/10.21203/rs.3.rs-2684100/v1. ...proteins derived from StayGold. ResearchSquare (Preprint). https://doi.org/10.21203/rs.3.rs-3188559/v1.... -
Viral Vectors 101: Chemogenetics
TypeBlog PostPublishedJuly 11, 2023, 1:15 p.m....chemogenetics in our chemogenetics guide Find viral preps for your research Find chemogenetics plasmids for... -
PhD Applications After COVID
TypeBlog PostPublishedJan. 5, 2023, 2:15 p.m....about your research goals and education, too! Prepping for the interview Many universities offer free... -
Polymerase Chain Reaction Overview and Applications
TypeBlog PostPublishedAug. 10, 2021, 1:15 p.m....Quantify RNA levels Molecular Diagnostics NGS library prep QC ddPCR Determine ultrasensitive and absolute... -
Hot Plasmids: Summer 2025
TypeBlog PostPublishedAug. 28, 2025, 1:15 p.m....highlight some of the new plasmids, antibodies, viral preps, and more in the repository through our Hot Plasmids... -
What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry
TypeBlog PostPublishedJuly 1, 2025, 1:15 p.m....Additional resources on the Addgene blog Plasmid Preps: Different Purity, Different Quantities, Different...Schlumpberger M. Effects of low A260/A230 ratios in RNA preparations on downstream applications. Qiagen Gene Expression... -
Hot Plasmids: Winter 2025
TypeBlog PostPublishedMarch 11, 2025, 1:15 p.m....some of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles...//doi.org/10.1038/s41477-024-01786-w. (bioRxiv preprint: https://doi.org/10.1101/2024.02.11.579803). ... -
Tips for a 1st time AAV user (by a Rookie AAV user)
TypeBlog PostPublishedOct. 23, 2018, 11:49 a.m....genome-containing viral particles that are present in an AAV prep. AAV samples are first DNaseI digested to remove...still necessary. To save time and money, I like to miniprep 5 mL of my AAV transfer plasmid cultures and then...pellet for the remainder of the cultures and later maxiprep only the cultures with intact ITRs. I use a spreadsheet...culture supernatant because it’s time consuming to prepare the 40% PEG solution. PEG takes several hours on.... Rather than investing all of this effort in preparing reagents, I choose to only harvest the cell pellet...separate out contaminants from an impure AAV preparation. 15%, 25%, 40%, and 60% iodixanol solutions are...double-check that the iodixanol gradient solutions were prepared correctly. When aliquoting purified and buffer... -
Neuronal labeling with Spaghetti Monster
TypeBlog PostPublishedAug. 14, 2018, 12:42 p.m....Addgene Browse all AAV vectors Find ready-to-use AAV preps ...STORM imaging, and electron microscopy. Sample preparation for these techniques sometimes makes it more ...retain their ability to be detected after sample preparation better than standard proteins tags like GFP. ...