Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 93 results for: tac promoter

Showing: 81 - 93 of 93 results

  1. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ...others understand ours. Improving access to data promotes dissemination, especially for young researchers...researchers who may be intimidated by having to contact more senior colleagues. Secondly, science is highly technical...

  2. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...encoded on the envelope plasmid which contains a promoter, the envelope gene, and a polyA tail.   ...protein’s role in infecting cell lines without using intact SARS-CoV-2 virus. Scientists have been using similar...

  3. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ...localization sequence) and expressing it from a CAGGs promoter. However FLPe is still less efficient than Cre... the Saccharomyces Cerevisiae 2 Micron Plasmid Attaches Covalently to DNA via a Phosphotyrosyl Linkage...

  4. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    Published
    Oct. 25, 2016, 2:30 p.m.
    ...help them navigate the scientific enterprise and promote their voices in driving changes in science. Follow...benefits may be changing. Please also feel free to contact us if you have information that you think would...

  5. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...and 3′ EGFP fragments under the ubiquitous CAG promoter. The approximately 500 bp genomic fragment containing...Now it’s time to consider where you would like to attack this gene. Should you cut it at the beginning of...

  6. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...enough to prevent confluency but high enough to promote cell growth. Overgrown cultures stimulate quiescence...mix Even before the lentiviral envelope protein attaches to cell surface receptors, other important binding...

  7. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ...non-interview setting. There are many resources on self-promotion (how hard it is for some people, especially women...Describe how you pushed to a solution or overcame obstacles. Take time to consider the order of your narrative...

  8. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ...integration at regions of open chromatin, especially at promoter or exonic regions. If the cell’s chromatin structure...piggyBac™, the cargo is inserted into the genome fully intact, while, with a standard DNA plasmid transfection...with any integrated transgene). To overcome this obstacle, a positive selection cassette can be incorporated...use of negative selection largely overcomes this obstacle. Furthermore, we have found that transfecting ...

  9. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ...RNA (protospacer+scaffold) in a pol-III (OsU3) promoter driven expression system. We use a single polycistronic...Ag-biotechnologist in the National Rice Research Institute, Cuttack, Odisha, India. Currently Kutubuddin is a Fulbright...

  10. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    Published
    Jan. 4, 2022, 2:15 p.m.
    ...When you go on interviews or are looking for a promotion, have some solid examples of times when you have...the past, or how completely different teams are tackling comparable conflicts. Is this level of domain ...

  11. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    Published
    March 14, 2017, 2:30 p.m.
    ...activates hypoxia inducing factor (HIF-1), which promotes the production of VEGF to increase angiogenesis...bolster and modulate our immune defenses to better attack cancer. Combing our knowledge with our bodies’ ...created by the cancer cells to shield themselves from attack. It is a region of incessant chemical activity,...that suppress APCs. T cells that would normally attack the cancer cells are also suppressed by chemical...microenvironment TGFβ, IL-10, can also help the immune system attack tumors. Attempts have been made to use whole cell...collaborative therapies must be explored. When attacking cancer, multiple immunotherapy targets can be ...

  12. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...nuclease-dead Cas9 (dCas9) is directed near the promoter of a target gene. Here, the target window is not...clonal heterogeneity may represent a more serious obstacle to the generation of truly isogenic cell lines...

  13. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...spectrum. When photons are absorbed by matter, they promote electrons within atoms to a higher energy state... analysis, you'll need to use fluorophores that attach to the target. When you design a flow experiment...cells with fluorophore-conjugated antibodies that attach to a specific target. By using a mix of different...
Showing: 81 - 93 of 93 results