...Biotin is a cofactor that in its active form is covalently attached to the active site of key metabolic ...in cells and is the process by which biotin is covalently bonded to a specific lysine at the active site...the bond between them is one of the strongest noncovalent protein-ligand interactions found in nature. ...in live mammalian cells with biotin ligase and monovalent streptavidin.” Nature Protocols 3.3 (2008): 534...

... in fusion proteins. Since GFP is such a well-validated, all-round good performing probe, it is the first...different acid concentrations. Overviews of the pKa values of different fluorescent protein are available ...remain fluorescent under acidic conditions, with pKa values below 4. Examples include mTurquoise2 (pKa = 3.1...Another peptide-based strategy uses an enzyme to covalently label the peptide with a fluorophore. Both systems...

...help of the DNA tuner program) (Latynski and Valentovich 2014). We routinely clone the purified amplicons...gentamicin-resistance (amplified from TP997). We validated these vectors, which we call “pTOX” in a broad...turn subtly blue or magenta. This is particularly valuable when first optimizing conjugation in strains that...PMID: 25800749. PMCID: PMC4513841. Latynski US, Valentovich LN. "DNA tuner: a computer program for the construction...

...off-target risk must be evaluated separately, increasing the time to clinical approval. Even with the challenges... in DMD patient myoblasts. They observed a 13% removal rate of exon 51, which resulted in appropriately...Pages Find CRISPR Plasmids for Your Research Find Validated gRNAs for Your Research ...

...work begin — validation, validation, validation! (psst… don't forget to submit your validation data to our...the mechanism of entry if known, and, as always, validate the capsid for your specific application and animal...Gore, B. B., Weed, N., Omstead, V., Bishaw, Y., Shapovalova, N. V., Martinez, R. A., Fong, O., Yao, S., Mortrud...

...using an antibody validated for your protein and application of interest or validating an antibody yourself...antibodies. Whenever possible, use validated antibodies or validate them yourself, so you don’t spend ...yourself. But even with a validated antibody, it can still take time for you to get it to work well. Dilutions...

...Epigenetics began as a correlative field in which covalent modifications to DNA or histones, the proteins...downstream of Tet1 to restore the DNA after cytosine removal. Recently, Albert Cheng’s lab developed Casilio-ME...lab has deposited pX330a dCas9-LSD1 for targeted removal of H3K4me1/2 and H3K9me2 histone modifications....Addgene.org Check out Our CRISPR Guide Pages Find Validated gRNAs to Target Your Genomic Locus of Interest...

...modification system and the scarless, Footprint-Free™ removal of cargo with the excision-only piggyBac™ transposase...stimulate homologous recombination and Footprint-Free™ removal of the selection cassette by excision-only piggyBac...site is left behind in the genome following donor removal, which alters the endogenous DNA sequence and has...nuclease thereby preventing re-cutting. Transposase removal of the cassette later restores the target sequence...

....twitter.com/drNBUdlQco — The Tarling-Vallim Lab (@TarlingVallim) December 19, 2020 In third place, the...shared by Hannah Drury (@druryhannah) The Tarling-Vallim Lab’s first place-winning entry doubled as their...

... the attainment of a bachelor's degree or its equivalent as a minimum (8 U.S. Code § 1184 - Admission ...must possess at least a bachelor's degree or its equivalent and state licensure, if required to practice ...beneficial to the United States For the purposes of evaluating an NIW, USCIS will also focus on the importance...field EB-1Bs historically have a high rate of approvals with USCIS due to the fact that the requirements...

...This time: E. coli genome-wide CRISPR inhibition Covalent tethering of DNA template to Cas9 SECURE base ...E. coli genome-wide CRISPR inhibition library Covalent tethering of DNA repair template to Cas9 Homology-directed...single-stranded oligodeoxynucleotide HDR template covalently attached to the Cas9-gRNA RNP complex via the...

...signaling pathways controlling properties like growth, survival, and differentiation.” https://www.addgene.org...frequently implicated in cancer cell proliferation, survival, differentiation, and apoptosis. For each of these...screen for] morphological features, migration, or survival in different contexts.” It’s also possible you...

...binding affinity (Janda et al., 2016; Torres & Casadevall, 2008) but…does it really do anything? Fc functions....2009.139 Janda, A., Bowen, A., Greenspan, N. S., & Casadevall, A. (2016). Ig Constant Region Effects on Variable...articles/10.3389/fmicb.2016.00022 Torres, M., & Casadevall, A. (2008). The immunoglobulin constant region...

...Once drug candidates are isolated, they can be validated for specificity to their target, or their direct...effectiveness prior to patient treatment. Target validation When a target is known and a drug is developed... to measure the specificity of that target and validate the hit compound. The standard is to test the ...

...have become a popular model organism because their larval stage lends itself well to studies of neuroscience...startled by acoustic, visual or tactile stimuli, larval zebrafish react by performing a distinct C-bend...in a transparent animal: circuit function in the larval zebrafish." Current opinion in neurobiology 23.1...

...organizations that share our community-oriented values. Addgenies like the nonprofit company culture as...gain access to new materials and information. Core values drive decisions at Addgene If you’re ever curious...making process at Addgene, just look to our core values. These accurately reflect our culture and outlook...

...antibodies are both extensively published, knockout validated, and work in your application. But…they are the...differences are subtle, chimeric antibodies should be revalidated before use. In addition, chimeric antibodies ...resources on the Addgene blog Antibodies 101: Validation Antibodies 101: Polyclonal Antibodies Antibodies...

...  Figure 3: Neutralization outcomes of convalescent sera with various pseudotyped virus. Image from...of SARS-CoV-2 501Y.V2 from neutralization by convalescent plasma. Nature 593:142–146. https://doi.org/...neutralizing antibodies predict disease severity and survival. Cell 184:476-488.e11. https://doi.org/10.1016...

.... The energy from this breakage is stored as a covalent bond between the cleaved 3′ DNA strand and a tyrosyl...different DNA strand comes along, it can attack this covalent bond thus joining the two DNA strands and releasing...overhanging 3´ deoxythymidine (T) residues that are covalently linked to topoisomerase. Vectors in these kits...

...Mentoring for Scientists series, you have: realized the value of having a mentor, developed some strategies for...Scientists Guide.  You can use it to clarify and discuss values. The activity is just the start – it is the discussion...inclusion, diversity, and open communication Feel valued by giving back Final tips for success Make mentoring...