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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 85 results for: yeast expression

Showing: 81 - 85 of 85 results

  2. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...bacterial expression vector which gives close to 100% cloning efficiency and gene expression. The pUCXKT... SFFV) or inducible (CMV-TO) cDNA expression, sgRNA (U6) expression, shRNA/shRNA-miR30 constitutive (H1...enhancing target gene expression anywhere from 87x to 20,000x over endogenous expression (depending on the...Testing your cell-free expression system: There’s a kit for that! Cell-free expression systems have been ...comparison of different cell-free expression systems; the Cell-free expression test kit represents a set of...benchmarking of cell-free expression systems, and tested for product integrity, expression level, and aggregation...fragments facilitates target DNA cleavage upon co-expression and spontaneous self-assembly. In an effort to...

  3. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...in mammalian and yeast cells using modified Cas9 proteins to regulate gene expression (CRISPRi/CRISPRa...step as simple as heat-shocking to induce the expression of a Cre recombinase contained within the knock-in...

  4. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ...Non-autonomous transposons, however, need another TE to express a transposase or reverse transcriptase so they ...size and is active in vitro as well as in vivo in yeast, plant, insect, and mammalian cells, including human...

  5. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...Cre-based Brainbow-expressing cells. Autobow plasmids are all-in-one versions which express self-excising ...multiple host cells in order to optimize expression. The expression plasmids for a given species are based...pMitoTimer from Yan’s lab, which is constitutively expressed, reports on the balance of biogenesis and mitochondrial...channel fluorescence images of C2C12 cells stably expressing pTRE-Tight-MitoTimer and rtTA. The cells were...down your endogenous protein of interest and co-expressing its replacement recombinant protein with a FKBP...deposited to Addgene, including lentiviral and AAV expression vectors for certain variants. Klapoetke et ... relatively long life-cycle of a protein from expression to degradation. Chemical approaches may require...
Showing: 81 - 85 of 85 results