...The principle of Tetbow In modern neuroscience, plasmid or viral vector-mediated gene delivery is more ... use Tetbow, you can introduce the four Tetbow plasmids (Addgene #104102 - #104105) using in utero electroporation...Tips and troubleshooting your Tetbow experiment Plasmid concentrations and AAV titer - The XFP genes have...achieve the highest color variations. Adjust the plasmid concentrations or AAV titer for the best results...

...urgent requests for access to coronavirus-related plasmids and to receive important coronavirus-related deposits...shipping staff agreed to come in and start getting plasmid deposits into the collection and to start shipping...support Addgene now, when their distribution of plasmids for COVID-19 research is accelerating essential...-related slowdown. More scientists are sharing plasmids and other reagents every day, and this crisis ...

...protein guide Find plasmids for subcellular localization Find fluorescent protein plasmids ...signal to noise ratio five fold over the 1xHA tag.  Lastly, the researchers found that the HA frankenbody ...the ability to bind nascent HA tagged proteins.  Lastly, the HA frankenbody can be used for imaging in ...

...at least four times more efficient in primary fibroblasts. iTOP also works in multiple types of primary...and gut stem cells, dendritic cells, embryonic fibroblasts, glia cells and neurons, and cell death post-... which transient protein expression has a long-lasting effect, such as Cre- or Cas9-mediated recombination...10.1016/j.cell.2015.03.028. PubMed. Find the Plasmids @Addgene: iTOP-tested proteins and reporters...

... waste of our time.  Networking connections can last years This is a graphic snapshot of my LinkedIn ...graduate school, there was only one other person in my class who had children. When I had my baby, we became ...the postdoc association. It was 25 years since our last contact, but we were delighted to be reconnected...years in many different ways. This leads me to my last tip: It’s usually not worth connecting with people...

...have yoga class twice a week at Addgene HQ, but now we’re sharing links to online yoga classes. Get outdoors...recently? Most of us at Addgene began working from home last week to flatten the curve, but working remotely ... Many studios and gyms are now offering online classes and participating in these is a great way to support...

...shyer classmates. For my undergraduate degree, I majored in human anatomy, but I also took classes in biochemistry...but adamantly refused to get them vaccinated. A classmate told me that they never finish a course of antibiotics...anxious, whispered questions from friends and classmates back in high school, and it’s oddly fitting that...

...their required components are very similar. While plasmids containing virus-specific elements such as the...integrate, and the addition of a helper virus or plasmid allows it to replicate episomally, essentially ...adenovirus cargo is high, it’s generally not as long lasting as AAV (up to several weeks vs. AAV’s several years...-associated Virus (AAV) Guide Addgene’s viral plasmids and resources Addgene’s viral services: AAV ...

...sequence for degradation. Viral introduction of shRNA plasmids can induce gene-specific silencing temporarily...transfection and random genomic integration of a plasmid. Viral transduction is also generally less toxic...response 2) very high immunogenicity 3) and long-lasting immune response even after a single dose. ...their use in other clinical applications. In the last decade, viral applications in cancer treatment and...

...specific mutations in your plasmids Use REPLACER Mutagenesis to mutate your plasmid Resources on Addgene.... targeted mutagenesis experiments? Find EvolvR plasmids at Addgene! References Gaj, Thomas, and Pablo...Browse our CRISPR guide pages Find bacterial CRISPR plasmids ...