... antibiotic resistance genes so that you can specifically isolate bacteria containing your constructs ...common antibiotic resistance genes and which applications are best (or worst) for each antibiotic. The...Ampicillin Ampicillin, often just called amp, is the historical king of the antibiotic resistance realm. Amp ...immediately after it has been heat-shocked or electrically zapped. As a result, a shorter recovery period...is expensive (relative to amp), and for some applications, the benefit it offers is marginal. Fortunately...antibiotic resistance ultimately comes down to your applications and needs. The more niche and specific your ... antimicrobial agents in MIC trays. Journal of Clinical Microbiology, 23(5), 959–961. https://doi.org/...

...translational applications (mechanistic studies and gene therapy treatments for neurological diseases). ...neuroscience research is the lack of a method for genetically manipulating neurons throughout the whole brain... creates a template that’s suitable for PCR amplification using pre-designed primers. By using this library...astrocytes. Why screen for IV deliverable AAVs? Typically AAVs are directly injected into the brain, but...throughout the body and is often difficult to surgically access. Therefore an IV injection is an ideal...pictures (see figure 4). It also enables single-cell anatomical study and neuronal tracing, aka determining where...

...that, they also help us keep track of the tubes’ physical location in the lab. These barcodes additionally...  freezers depending on the sample type. It is critical for us to know which plate, rack, and freezer ...missing so that we can make a new tube from a replicate sample. The transformation After the DNA has ...plasmid can grow. As the bacteria divide, they replicate and retain the plasmid. This allows us to make...help us track each sample, but we also have a dedicated and detail-oriented lab team that works carefully...performs an average of 140 transformations. The lab typically transforms newly deposited samples from Monday...Excel sheet.3. Be specific  - When assigning a physical location to your samples, include as much detail...

...Summer Science Communication Series. We’ll be covering the basics of science communication, tips and tricks...science with others, and introducing science communication as a research field. And there will even be ...be a few tips for scientific communication (manuscripts, talks, etc…!)  Deep Dives You probably are familiar... Dives” series comes in! We’ll take a common application or concept and go beyond the basics - diving ...you’re ready for the next steps on some common applications, join us as we get into the weeds on some of...

...expression limiting regulatory elements or use a non-canonical code (Gustafsson et al., 2004), such as Hoogsten...folding and stability, change post-translational modification sites and even affect protein function (Mauro...recognize multiple synonymous codons) may actually be critical for determining the rate of translation. The ribosome...10.1101/gr.139758.112 Mauro VP, Chappell SA (2014) A critical analysis of codon optimization in human therapeutics...synonymous codon usage bias, and its potential applications. Nucl Acids Res 15:1281–1295 . https://doi.org... GluCl ion channel genes for mammalian cells dramatically improves expression levels. Journal of Neuroscience...synthetic? How Addgene;s dataset reveals trends in biological innovation Resources on Addgene.org Check out...

... gives you a single level of control without modification - either Cre is there or it’s not. Cre-mediated...37 °C). Smart molecular evolution led to the identification of FLPe which has a temperature optimum of ...asymmetric target site core but its 8 bp length that is critical for Cre and FLP function. Because recombination...Recombination between the two target sites with identical sequence leads to inversion of the flanked DNA...this plasmid mix results in sparse labeling of cortical neurons and their synapses.   How can you...control   Figure 2: Expression of a morphological marker (eYFP) and synaptic marker (PSD95-mcherry...(7): 657–62. PubMed PMID: 9661200.  3. Cardin, Jessica A. et al. 2009. “Driving Fast-Spiking Cells Induces...

... this post I’d like to delve deeper and look specifically at the characteristics and capabilities an ELN...facilitate true reproducibility. The following are critical: (1) connectivity to other data sources used in...likely stored in institutional and lab servers specifically designed to hold large amounts of data. Although...group’s research – it makes no sense and is not practical to move all this data in bulk into a lightweight...lightweight tool like an ELN. It is nevertheless critically important to be able to conveniently reference specific...with a particular experiment or project thus significantly enhances the ability of others to subsequently... both accept deposit of data associated with publications into one of five general purpose open source...

...described above showed successful target modification, indicating that AAV is a good delivery vehicle for...overall packaging capacity but necessitating purification and co-infection of two AAVs. Cas9 orthologs...post-injection. Livers from these mice were histologically normal and liver injury markers were not increased...the AAV-SaCas9-sgRNA constructs mediate genome modification, but they did so without a substantial immune...enough to deliver with AAV. This system fuses catalytically dead dCas13b to the catalytic domain of RNA ...finding smaller Cas9 orthologs works for some applications, it doesn’t eliminate the need to deliver larger...

...parenting strategies and make important ecological indicators since species live in water and on land....protocol community! Asclepias syriaca and Asclepias curassavica (Gerog Jander) Milkweed plants are known to many... as the only Monarch butterfly food. They have chemical pathways generating interesting drug-like compounds...Lab (@EvoEmbryo) September 24, 2018   Xenopus tropicalis (Gary Gorbsky) This is not a new experimental...plants and are unusual enough to be interesting biologically. They have economic importance as harmful weeds... expensive. They are using a cool fluorescent indicator system to rapidly identify recombinants. Arthropods...

...very little data on which might work in your application. It can be quite difficult to decide on which...be searched and sorted by gene/target, name, application, pass/fail rating, sample species, antibody species... how the antibody performed in the submitted application, along with a brief description of the experiment...experiment. If that data was included in a publication, you'll find that information in the report as well... section will vary slightly depending on the application — flow cytometry, western blot, immunohistochemistry...data showing how the antibody worked in your application. The submission process is easy, with simple,...