We narrowed to 884 results for: ica

...progress on establishing a system for the chemical replication of RNA in the absence of evolved enzymatic...enzymatic machinery (i.e. RNA replication with no proteins). The chemistry is complex and this work requires...inheritance that might be less error prone than DNA replication. He too mentioned how “surprising” small RNAs... about using CRISPR/cas to address the growing clinical problem of antibiotic resistant bacteria. See ... and Sun Yat-sen University presented two new technical developments. Using the Tet-on system, his lab...Steitz quipped that RNA in the ribosome is in a complicated conformation “not like DNA which has a boring...

...Because of their small size, nanobodies can be synthetically expressed off of plasmids in bacteria, and they...with a large and diverse pool of sybodies that hypothetically contain ones that bind strongly to your target...Starting with libraries and a biotinylated (for purification purposes) protein target of interest, the protocol... identifying sybodies that bind strongly and specifically to the protein of interest. The Seeger lab used...-30) of sybody candidates are sequenced for identification. All unique sybodies can be subcloned into ...2020) Generation of synthetic nanobodies against delicate proteins. Nature Protocols 15:1707–1741. https...Light to Manipulate Cell Signaling and Protein Purification RANbodies: Reporter Nanobody Fusions Plasmids...

...McDonald. "A practical guide to evaluating colocalization in biological microscopy." American Journal of... proteins of interest and examine them using biochemical methods thus removing the context of the cell...way it is important to choose the appropriate quantification methods and tools. It is usually not enough...microscopy is limited by the wavelength of light and, practically speaking, for common lab microscopes the detection...Quantitative analysis of dynamic association in live biological fluorescent samples." PloS one 9.4 (2014): e94245...

... (green or blue) within a population of timer indicates the age of the pool: the higher the ratio, the...fluorescence falls within the linear window for the theoretical timer shown in Figure 1B, you can use the equation...-FT-N1 pTRE-Slow-FT pBAD/HisB-Slow-FT -  Applications of fluorescent protein timers FP timers have...protein, or cellular structure and a particular biological event (trafficking to a subcellular location,...of neural fiber bundle formation." Journal of Biological Chemistry 276.32 (2001): 29621-29624. PubMed ...to red report on cellular trafficking." Nature chemical biology 5.2 (2009): 118-126. PubMed PMID: 19136976... insulin-secreting beta-like cells." Nature communications 7 (2016). PubMed PMID: 27063289. PubMed Central...

...requires incubation at 55 °C - a dangerous, non-physiological temperature for mammalian cells and one which...been excitedly testing NgAgo's genome editing applications. So, how does NgAgo stack up thus far? A few...survey. When asked if they could detect indels (an indication of gene disruption ability) with NgAgo, one respondent...could not achieve indels with the NgAgo system indicates that it is very tricky to use and fussy to optimize...proposed in the original article, to achieve greater efficacy, researchers will have to carry out repeated transfections...results out there include: Google Groups - I've specifically set up a forum where NgAgo users can share protocols... S. Lander, and Feng Zhang. "Development and applications of CRISPR-Cas9 for genome engineering." Cell...

... most commonly used are GECIs - genetically encoded calcium indicators (e.g. GCaMP). Others include: pHluorin...capacity. Many of these FPs are fluorescent when ectopically expressed in most organisms. Furthermore, fusing...fluorescence. Therefore, FPs are used to study many biological questions. The two most common uses are: 1) to...For LSS proteins, the Stokes shift is ≥ 100nm. Specifically, these proteins are excited by UV light or blue...were prone to oligomerize. This may affect the biological function of the FP-fusion protein. Therefore,.... al. (2010) “Fluorescent proteins and their applications in imaging living cells and tissues”  Physiol...

...excited to announce we now offer an export license application for rabies materials! Some of the materials in...international requestors. We also offer export license applications for VSV and SARS-COV-1 plasmids. Export control...will need to provide a brief description of the applicable research project when purchasing export-controlled... applied to cover processing costs. Once the application is approved, Addgene then ships the material ...offered VSV and SARS-COV-1 plasmids under this application process since May 2020. We find this process ...whenever possible, as we cannot expedite the application process.  More information about export control...

...translational applications (mechanistic studies and gene therapy treatments for neurological diseases). ...neuroscience research is the lack of a method for genetically manipulating neurons throughout the whole brain... creates a template that’s suitable for PCR amplification using pre-designed primers. By using this library...astrocytes. Why screen for IV deliverable AAVs? Typically AAVs are directly injected into the brain, but...throughout the body and is often difficult to surgically access. Therefore an IV injection is an ideal...pictures (see figure 4). It also enables single-cell anatomical study and neuronal tracing, aka determining where...

... antibiotic resistance genes so that you can specifically isolate bacteria containing your constructs ...common antibiotic resistance genes and which applications are best (or worst) for each antibiotic. The...Ampicillin Ampicillin, often just called amp, is the historical king of the antibiotic resistance realm. Amp ...immediately after it has been heat-shocked or electrically zapped. As a result, a shorter recovery period...is expensive (relative to amp), and for some applications, the benefit it offers is marginal. Fortunately...antibiotic resistance ultimately comes down to your applications and needs. The more niche and specific your ... antimicrobial agents in MIC trays. Journal of Clinical Microbiology, 23(5), 959–961. https://doi.org/...

...that, they also help us keep track of the tubes’ physical location in the lab. These barcodes additionally...  freezers depending on the sample type. It is critical for us to know which plate, rack, and freezer ...missing so that we can make a new tube from a replicate sample. The transformation After the DNA has ...plasmid can grow. As the bacteria divide, they replicate and retain the plasmid. This allows us to make...help us track each sample, but we also have a dedicated and detail-oriented lab team that works carefully...performs an average of 140 transformations. The lab typically transforms newly deposited samples from Monday...Excel sheet.3. Be specific  - When assigning a physical location to your samples, include as much detail...