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We narrowed to 814 results for: LAS

Showing: 511 - 520 of 814 results

  1. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    Published
    April 10, 2018, 12:56 p.m.
    ... a reporter and a nanobody that’s encoded on a plasmid. A nanobody is a ~150 amino acid, single domain... your own RANbodies? Great! All of the RANbody plasmids from Yamagata et al can be found here. Can’t find...consider making you own DNA ladders with these plasmids from Penn State Need help picking the right fluorescence...Resources on Addgene.org More nanobody expression plasmids can be found here Watch this video to learn more...

  2. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ...Fluorescent protein plasmids Use FRET to study protein-protein interactions Deposit plasmids with Addgene! ...visualizing protein turnover in situ in neurons. The classic approach for studying protein turnover is radioactive...Tagged protein can easily be overexpressed from a plasmid but overexpression may interfere with the system...

  3. How to Prepare for an Industry Interview

    Type
    Blog Post
    Published
    July 9, 2020, 1:15 p.m.
    ...starting with the hiring manager. These interviews lasted for 30 minute to an hour. Although I never experienced...prior research project. In total, the interviews lasted from half to all of the day.  This whole process...technical difficulties. Have your presentation on a flash drive and consider bringing your own laptop as a... is complete Industry interviews are long! Mine lasted 4.5 hours, which was a lot of extraversion for ...

  4. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ...popular split-FP plasmids from our catalog to help you find the right split-FP plasmid for your experiment...below, and click the Article link to find all the plasmids from the associated paper:   Color Base FP...and experimental design. We hope you find these plasmids useful. Good luck, and happy experimenting! References...

  5. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...underway to explore CRISPR’s capacity for both classes of edits in cancer therapy. Blood disorders Two...Disease (SCD) and Transfusion-dependent beta thalassemia (TBT), both of which affect hemoglobin. Most ...introducing a desired edit, but this particular class of treatments has quite the opposite aim: to destroy...ijms24021563 Resources on Addgene.org CRISPR Plasmids and Resources CRISPR 101 eBook CRISPR History ...

  6. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ...Want to get your hands on SpRY? Find a mammalian plasmid here and edit on! References and Resources References..., 6175 (2023). DOI:10.1038/s41467-023-41829-y (Plasmids available here) Walton, R. T., et al., Unconstrained...368,290-296(2020). DOI:10.1126/science.aba8853 (Plasmids available here)   Nishimasu, H., et al.,Engineered... on Addgene.org Addgene’s CRISPR Guide CRISPR Plasmids and Resources Resources on the Addgene blog ...

  7. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ...for expression of a gene carried on the transfer plasmid or quantifying the number of viral copies that ...beneficial as they ensure that the correct transfer plasmid was used in the transfection; if preparing several... that comes from amplification of the original plasmid which can over-inflate results. A packing vector...antibiotic resistance genes carried on transfer plasmids. Target cells are transduced with serial dilutions...

  8. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...Agarose Method Plasmids 101: Mammalian Vectors Resources on Addgene.org Find mammalian plasmids for your ... It’s good practice to label your plates and/or flasks with your name, date, the cell line you’re using...records of your experiments. Autoclave is the rave! Last, but not least, any lab material that is intended...

  9. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...resources on addgene.org Find Optogenetics Plasmids Find Biosensor Plasmids Visit the Fluorescent Protein Guide...Frank Hayward et al., 2024). They deposited their plasmid with Addgene, hoping to inspire others to explore...Lab is eager to hear from you. Find the MagLOV plasmid here!   References and resources References Duret...

  10. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    Published
    Aug. 3, 2023, 1:15 p.m.
    ...tool for those wishing to run larger panels: five laser spectral flow machines have 64 detectors, allowing...spectra for two fluorophores excited by the violet laser, BV421 and BV711. These “rainbow box” plots are ...full emission spectra for BV421 and BV711 on a 3 laser spectral flow cytometer   Using spectral flow...Similarity index across 12 different colors on a three laser spectral flow cytometer.   Level of co-expression...
Showing: 511 - 520 of 814 results