... serotypes in order to create an atlas of the mouse brain. This atlas enables researchers to quickly find...streptavidin-binding strep-tag and, more recently, OLLAS (Escherichia coli OmpF linker and mouse langerin...in particular are difficult to track with these classic probes. To overcome the limitations of existing...

...their MECP2-related plasmid tools with Addgene and share additional resources. Plasmid tools to facilitate...to partner with them to launch a Rett Syndrome Plasmid Collection and Resource Center for Rett Syndrome... Rett Syndrome in novel ways.  Addgene’s MECP2 Plasmid Collection and Resource Center will continue to...

... usage bias in Plasmids 101: Codon usage bias Read more about plasmids in our Plasmids 101 blog series...Reference Read about genetic code expansion Browse our plasmid collection page  ...

... at a particular hole (the active hole) induces laser stimulation of the chosen brain circuit, while a...most of its times in the opposite chamber) [2]. Laser activation is usually controlled automatically using...naturally so, after all the optimization, turn on the laser and watch what happens. Who knows, you may observe...optogenetics setup (viral expression, efficiency of laser light transmission to the target neurons, appropriate...

...used pairs to measure FRET. The table below lists plasmids that can be used to create your choice of fluorescent... fusion protein with your gene of interest: Plasmid Color Expression Description pPROEX Aqua Cyan...can easily directly excite the acceptor with the laser used to excite the donor. In that case the cross-talk...CRET and other RET techniques which have never outclassed the Master. Note: BRET= Bioluminescence Resonance...

...to whole-body in small animals, such as mice (Matlashov et al., 2020). Enhanced miRFPs are monomeric and...NIR FPs named miRFPs (monomeric infrared FPs) (Matlashov et al., 2020) derived from bacterial phytochrome...multicolor NIR imaging.  Small miRFPnano A separate class of NIR FPs, named miRFPnanos, were derived from ...ion indicator. Nat Methods 16, 171-174 (2019). Matlashov, M.E. et al. A set of monomeric near-infrared ...

...ever watched any behind the scenes footage in the last several years, you can usually see a director sitting...the subject after I’ve left the room. Microphone: Lastly, I needed to figure out a microphone situation....beating heart of Addgene. It’s why we exist, storing plasmids, and distributing them to labs that need them....a different shoot to create the How to Deposit Plasmids video. Shot without anyone else in the office....

...techniques comes in handy whether you are perusing classic papers or are brainstorming new technological innovations...blotting, restriction mapping and sequencing gels are classic techniques that allowed for many seminal scientific... on Analyzing DNA Sequencing Results Check out Plasmid Cloning by PCR Resources on Addgene.org Check...Pages Find Molecular Biology Tools Analyze Your Plasmids by Restriction Digest ...

...and quality controlled plasmids and viral vectors. When working with a plasmid from Addgene, you can be...Last Wednesday we worked with the Harvard GSAS Science Policy Group to organize a Minisymposium on Reproducibility...Sharing - Susanna Bachle, Addgene the nonprofit plasmid repository 49:45 0 1:18:45 - Reagent Development...

...finishing my MSc. The masters program was 6 months of classes and 6 months of hands-on lab work for a dissertation...would like to do. I had taken advanced virology classes in California and enjoyed them, so I applied for...found out the job was with Addgene. I had used plasmids from this company before and recognised the logo...experiments over the phone or explain how various plasmids work. Most people who call or email me are scientists...