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We narrowed to 814 results for: LAS

Showing: 521 - 530 of 814 results

  1. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...Antibodies 101: Monoclonal Antibodies Plasmids 101: Peptide Tagging Plasmid-based Recombinant Antibody Resources.... In two studies investigating mechanisms of endoplasmic reticulum localization, Munro and Pelham casually... recombinant anti-c-Myc [9E10] at Addgene Find plasmids with the myc-tag  ...

  2. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    Published
    Aug. 3, 2023, 1:15 p.m.
    ...tool for those wishing to run larger panels: five laser spectral flow machines have 64 detectors, allowing...spectra for two fluorophores excited by the violet laser, BV421 and BV711. These “rainbow box” plots are ...full emission spectra for BV421 and BV711 on a 3 laser spectral flow cytometer   Using spectral flow...Similarity index across 12 different colors on a three laser spectral flow cytometer.   Level of co-expression...

  3. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...the complex is docked on the membrane of the endoplasmic reticulum. And graduate students, momentarily...a surface format made of rock-hard, inflexible plaster. As a result, the model obscures the flexible nature...Designs web site. Or, if you are visiting a local classroom or trying to explain your science to the general...Guide Page CRISPR/Cas9 History Browse All CRISPR Plasmids and Resources ...

  4. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ...school system that has made strong efforts in the last 5 years to incorporate more science and technology...page in terms of the vocabulary--DNA, protein, plasmid, gene, etc. Though I talked about CRISPR’s molecular...should we use CRISPR? How shouldn’t we use it? This last section was arguably the most important, and it ...out our comprehensive CRISPR guide Find CRISPR plasmids and resources ...

  5. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ... donor DNA, and avoiding common mishaps in this class of genome edit. Knock-in mutations and gene editing...Targeted genome editing events fall into two general classes: knock-in and knock-out. The goal of a knock-out...experiment. Also learn about Addgene’s knock-in plasmids for reagents to help you knock your knock-in experiment...Cells Resources on Addgene.org Browse All CRISPR Plasmids Find Validated gRNAs for Your Next Experiment ...

  6. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...vectorization strategies include creating an AdV transfer plasmid with the E1 and E3 (Early 1 and Early 3) genes .... These genes’ functions are rescued by helper plasmids used during production and/or by genes present...made available by this deletion from the genomic plasmid can be used to house a recombinant sequence (transgene...late genes are usually present in the transfer plasmid for the majority of adenoviral vector systems used...

  7. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ... serotypes in order to create an atlas of the mouse brain. This atlas enables researchers to quickly find...streptavidin-binding strep-tag and, more recently, OLLAS (Escherichia coli OmpF linker and mouse langerin...in particular are difficult to track with these classic probes. To overcome the limitations of existing...

  8. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    Published
    Nov. 19, 2020, 2:15 p.m.
    ...their MECP2-related plasmid tools with Addgene and share additional resources. Plasmid tools to facilitate...to partner with them to launch a Rett Syndrome Plasmid Collection and Resource Center for Rett Syndrome... Rett Syndrome in novel ways.  Addgene’s MECP2 Plasmid Collection and Resource Center will continue to...

  9. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ... usage bias in Plasmids 101: Codon usage bias Read more about plasmids in our Plasmids 101 blog series...Reference Read about genetic code expansion Browse our plasmid collection page  ...

  10. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    Published
    Oct. 1, 2015, 2:30 p.m.
    ... at a particular hole (the active hole) induces laser stimulation of the chosen brain circuit, while a...most of its times in the opposite chamber) [2]. Laser activation is usually controlled automatically using...naturally so, after all the optimization, turn on the laser and watch what happens. Who knows, you may observe...optogenetics setup (viral expression, efficiency of laser light transmission to the target neurons, appropriate...
Showing: 521 - 530 of 814 results