...University and Medical Center  Weizmann Institute of Science Whitehead Institute for Biomedical Research ...time of request MTAs and deposit agreements to basically instantaneous in many cases.  Suggest that they...your organization below? Don’t worry--we check periodically for possible new awardees, and we’re happy to...University Rockefeller University  Salk Institute for Biological Studies The Scripps Research Institute St. Jude...Massachusetts, Worcester  University of Nebraska Medical Center University of Pennsylvania  University of...

...Addgene! Archon1, a voltage sensor AAV Historically, electrical activity in the brain was measured with...kinetics) that makes them suitable for different applications. We recently released a new variant of jGCaMP7... been developed and are suited for different applications. The iGABASnFR GABA sensor (pAAV.hSyn-FLEX.iGABASnFR...voltage reporter allows a way to monitor neuronal electrical activity through imaging. Since it’s illuminated...Also coming in our new 20 ul trial size! Voltage indicators Plasmid Serotype Name 119036  AAV1  pAAV-hsyn-flex-Voltron-ST...

...the gRNA design process and rules for various applications is necessary going forward. The standardization...benefits to researchers conducting basic or early pre-clinical research. However, standard terms and techniques... I think I may have finally grasped just how complicated DNA repair pathways are. I fully admit to being...to me. But coming to #KSgenome and seeing the intricacies of DNA repair has emphasized how essential these...Alt-NHEJ), has been harnessed for CRISPR knock-in applications. CRISPR 101: Targeting RNA with Cas13a (C2c2...for CRISPR Genome Editing Other resources and publications CRISPOR gRNA design tool Researchers at the ...

...infection, because this setup allows for experimental replicates for each of the gRNAs. The idea was to assign...genetic tool for labeling just a fraction of a genetically-defined subset of cells. By labeling fewer cells...intermediate (d), and sparse (e) labeling of the calcium indicator, GCaMP6f, in the Drosophila optic lobe. Image ...measurements, or monitoring in vivo. To overcome these technical issues, Sabrina Büttner’s lab designed a calcineurin... reporter (blue) and stained with PI (pink) to indicate cell death. Image from Diessl et al., 2020. ...protein to the DNA. They created plasmids for purification of tTALE-eGFP and tTALE-mCherry. In comparison...

... (AmpR) and origins of replication (ori and f1 ori) which allow it to replicate.   How can I prevent...the bacterial selection marker and origin of replication. During bacterial culture, only the plasmid with...the antibiotic resistance gene and origin will replicate, and the transgene will be lost. Over time, bacteria...the recombined plasmid is still visible. This indicates that the DNA prep contains a mixture of the full...MK, Rudolph C (2019) Segmented poly(A) tails significantly reduce recombination of plasmid DNA without ...

... “species on species”) immunochemistry (IHC) applications an issue? The culprits are endogenous IgGs in...IgGs and your primary antibody, resulting in significant background staining (Figure 1A). Not all tissues...Figure 2B) Some labs have found that you can significantly decrease background staining by mixing your ...Randolph-Habecker J (2013). A Flexible Mouse-On-Mouse Immunohistochemical Staining Technique Adaptable to Biotin-Free...Partridge TA (1998). A New Blocking Method for Application of Murine Monoclonal Antibody to Mouse Tissue...Tuson JR, Pascoe EW, Jacob DA (1990) A novel immunohistochemical technique for demonstration of specific binding...

...lab used an alternative strategy to develop a genetically encoded antibody based probe that detects the...antibodies for extracellular and intracellular applications: CDR grafting to stable frameworks and structure-based...National Academy of Sciences of the United States of America, 69(9), 2659–2662. PubMed PMID:4560694. PubMed ... and antibodies with crystalline papain. The Biochemical Journal, 73(1948), 119–126. PubMed PMID: 14434282..., T., Sato, Y., … Stasevich, T. J. (2019). A genetically encoded probe for imaging nascent and mature ...mature HA-tagged proteins in vivo. Nature Communications, 10(1). PubMed PMID: 31270320. PubMed Central PMCID...

...Harvard Medical School and runs everything science (CSO). Clinton is a cost analyst at Dow Chemical (a Fortune...a company in the health sciences without those critical three letters after your name (P-H-D).  Finally...two projects, including one led by a talented biomedical engineering team from MIT.   I would like to ...launching a company without partners.  You need a dedicated team that you know well and trust.  This will ...majority of these meetings acted as a platform for significant company advancement. Be relentless The world ...

...databases available, Web of Science. For each publication, Web of Science provides basic bibliographic ...Microbiology, Biochemistry & Molecular Biology, and Biochemical Research Methods. We also pulled the big interdisciplinary...Science, PLOS One, and PNAS. We then broke the publications from these journals into two groups: those containing...the papers with plasmids deposited at Addgene – typically about four times as many as papers without plasmids...differences in citations due to the journal of publication. Table 1 provides an example calculation of the...Addgene in them. The adjustment is done within publication years. What do we find once we do this adjustment...

...field has exploded so quickly, citing the first clinical trial with iPSCs with his collaborator Takahashi... have profound impacts on the development of applications with the potential to save lives. While most...picture perspective: Dr. Yamanaka talked about his clinical trial patients with advanced macular degeneration... ground-breaking work on spinal cord injuries; Jessica Young shared research on stem cells and Alzheimer's...Eggan presented work that is leading to promising clinical trials for ALS.   I have an uncle with Parkinson's...the first stem cell line that could be used in clinical trials. The quality control process alone takes...