We narrowed to 772 results for: INA

1. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

   Type

   Blog Post

   Published

   May 11, 2021, 1:15 p.m.

   ...easy detection with the appropriate fluorescent illumination in in vivo, ex vivo, or post-mortem experiments...Yerkes National Primate Research Center. Using a combination of in vivo and anatomical techniques, her research...

2. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

   Type

   Blog Post

   Published

   April 13, 2017, 2:30 p.m.

   ...expressed. Pho8Δ60 localizes to the cytosol in an inactive form unless autophagy is induced. Then non-selective... Pho8 from its uncleaved to cleaved form is the final read out of this assay. This is also a quantitative...

3. Seven Tips for Using LinkedIn as a Scientist

   Type

   Blog Post

   Published

   Oct. 1, 2019, 1:21 p.m.

   ...LinkedIn may revoke your ability to send invitations. Finally, if you don’t know clearly why you are inviting...updates, or click bait of any kind. For more on inappropriate content see this article. However, do comment...

4. Troubleshooting Your Plasmid Cloning Experiment

   Type

   Blog Post

   Published

   Sept. 24, 2019, 12:55 p.m.

   ... DNA that you want to assemble together in a combination of different ways according to your cloning strategy... or PCR products from a gel. You will avoid contamination from other DNA fragments and you will remove...

5. Reproducibility for Everyone: Lessons from an Open Science Collaboration

   Type

   Blog Post

   Published

   Feb. 28, 2019, 1:34 p.m.

   ... during, and after organizing such a workshop. Finally, the group put a lot of effort into ensuring that...them can do more harm than good. Check out this webinar for more on this. Be on the lookout for upcoming...

6. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

   Type

   Blog Post

   Published

   Sept. 4, 2018, 12:53 p.m.

   ...plasmid copy number in a cell. One can attempt to eliminate fluctuations due to copy number by introducing...multi-protein complex, stabilized promoters can eliminate variability between the different components. ...

7. Addgene's Tips for Plasmid Quality Control

   Type

   Blog Post

   Published

   Jan. 14, 2016, 3:30 p.m.

   ... (such as LTRs or ITRs), hairpins, or strong terminators. Detailed instructions on other factors to consider...when designing your primers can be found here. Finally, you will want to have a well-organized database...

8. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

   Type

   Blog Post

   Published

   Dec. 16, 2015, 3:30 p.m.

   ...combine the best of these mutations and tested the combination mutants for their on-target and off-target activity...that eSpCas9(1.1) and SpCas9-HF1 each stall in an inactive form after binding to a target site and propose...

9. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

   Type

   Blog Post

   Published

   May 5, 2015, 3:54 p.m.

   ...was contributed by Johnna Roose. This post was originally published on Johnna's New Under The Sun Blog ...In others, the experiments are geared towards eliminating possibilities until the results focus in on a...

10. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

    Type

    Blog Post

    Published

    April 22, 2015, 2:06 p.m.

    ...complexity. We have characterized almost 600 terminators. That gives us enough hardware to control hundreds...over the next year. Then there will both be a combination of parts in Addgene along with the software to...