...Gonçalves and his colleagues at Leiden University Medical Center are now available at Addgene along with ...and very efficient introduction of DNA into therapeutically relevant, non-transformed mammalian cells. ...and quiescent, post-mitotic mammalian cells. Applications of CRISPR delivery by adenoviral vectors Since...and used AdV vectors to edit genes in several biological systems. It has been used for instance in inherited...Strategies for Tissue-Specific Targeting. In: Applications of viruses for cancer therapy. Elsevier, pp ...Viral vectors for gene therapy: Current state and clinical perspectives. Biochemistry Moscow 81:700–708 ....

...experts in science communication in real time! I follow great online science communicators like Phil Plait...nontraditional” career path long ago. Use your analytical mind to test and discover what career suits you...about any topic I like and gain experience in communication, editing, marketing, and website organization.... She is particularly interested in science communication and fundamental molecular biology.      Additional...  science communication...

... familial aspect of Addgene. As our size has drastically increased, it would have been a tight squeeze...event! Addgene company culture At Addgene we organically developed the philosophy of  “work hard, play...department) right in your breakroom. 5. Throw some physical activity into the mix It’s easy to sit around ...be an obvious one, but don’t make things too complicated for yourself (especially if you are the sole ...sole event planner)! If you have a very intricate itinerary, there is a higher likelihood that something ...Delegate- it will save you time and sanity!    10. Communicate Make sure people know that there is something...

...regions and allocate specific functions to them. Electrical recordings and lesioning studies have been essential...within the LC of mice. When those neurons are chemogenetically activated, norepinephrine release causes a...test has been used since the 1970s to test the efficacy of antidepressant treatment in rodents. In this...responsible for the antidepressant effect. To specifically activate the Drd1 neurons, they injected AAV2... "Hypothalamic CNTF volume transmission shapes cortical noradrenergic excitability upon acute stress."...long-lasting antidepressant effects." Nature communications 10.1 (2019): 223. PubMed PMID: 30644390. PubMed...

...Societies, such as the American Society for Cell Biology (ASCB) or the American Chemical Society (ACS) are ...often scarce and difficult to identify. So what specifically should you do? What follows are just a few suggestions... in fact, some of the very things that were so critical to me landing my own position! Conferences One...know all in one place! This information can be critical to a focused and effective job search. As with...

...possess cell walls. As a result, Mycoplasma are intrinsically resistant to this class of antibiotic. Acquired...Microbes (bacteria included) are very skilled at the delicate art of survival. Existing virtually ubiquitously...of intentions. The take-home? Always take your medication as prescribed. How do we know which bacteria ...When antibiotic resistance testing is requested clinically, this involves growing a standardized amount ...-trusted method that is frequently used in the clinical laboratory. What can we do to stop the emergence... is passionate about health equity, science communication, and public health.     References 1. O'Neill...

...development happening at Addgene under the hood. A significant portion of our development may not be visible...ourselves. We are also able to consider different technical approaches that may come up during discussion.... information. It is quite possible that the clarifications lead to the story being scrapped or broken ...While writing the code, we may need additional clarification and again we collaborate with the Product Team... been updated in several years) or they make significant changes that make it incompatible with our software...software from Python 2.7 to Python 3.5. There are significant changes to the language and it took us a total...

...FRET during a journal club, my guitarist brain automatically thought about the raised element found on the...allows the detection of molecules' interactions, modifications or dissociations in situ. Used since the mid...interactions in living cells using a microscope. Historically, molecular interactions were detected by indirect...Periasamy defined FRET as "a distance-dependent physical process by which energy is transferred non-radiatively...highest absorbing acceptor and fluorophores with significant overlap in their spectra. The pair CFP-YFP was...14. Zimmermann T,  Terjung S. FRET Basics and Applications: An EAMNET Teaching Module http://www.embl.de...

...resistance gene and the origin of replication are necessary to replicate plasmids and make sure the cells... promoters only work in prokaryotic cells and typically only in the same or closely related species from... be ways to turn them on or off at will with a chemical, heat, or light. In the cell, promoters themselves...scientists have created synthetic promoters, which typically include some combination of other promoter elements...Inducible with Tetracyline or its derivatives.  Typically contains a minimal promoter with low basal activity...

...percentage of survey respondents indicated that cost was a significant barrier to ELN adoption. This includes...priorities in most cases include IP protection, publications and funding. If any of these three pillars crashes...to digital for example): “… 99% of respondents indicated that ease of use would influence their ELN choice...collaborative functionalities include comments, notifications, sharing files, assigning team members to certain... step of the research process will save them significant amounts of time and effort and give the lab a...