We narrowed to 814 results for: LAS
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TypeBlog PostPublishedJune 19, 2018, 1:38 p.m....Addgene.org Find plasmids for in vivo imaging with fluorescent proteins Browse luciferase plasmids Find fluorescent...acoustic reporters for your research? arg1 and arg2 plasmids are available from Addgene! References 1 Bourdeau...
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CASTing Off for New Shores in Human Genome Editing
TypeBlog PostPublishedJune 10, 2025, 1:15 p.m....moved a gene required for phage propagation to a plasmid that lacked a promoter — only CAST integration ...integrate DNA payloads of up to 15 kb. Find evoCAST plasmids here! The new horizons of evoCAST In the emerging...Addgene's CRISPR Guide (Jump to Large Edits) CAST Plasmid Collection ... -
Antibodies 101: Fc Effector Functions
TypeBlog PostPublishedMay 18, 2023, 1:15 p.m....These receptors are specific to isotype class or even subclass, and are part of the reason why different...antibodies on a pathogen activates the immune system's classical complement pathway, starting a cascade of events... -
Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science
TypeBlog PostPublishedAug. 8, 2019, 1:19 p.m....depositing plasmids pre-publication Find molecular biology protocols Find eBooks on plasmids, CRISPR, viral...community. Be the peer-reviewer you wish for The last thing that I wanted to mention about the peer-review... -
Single-cell tracking of lineage and identity with CellTag
TypeBlog PostPublishedMay 9, 2019, 1:15 p.m.... the direct reprogramming of mouse embryonic fibroblasts (MEFs) into induced endoderm progenitors (iEPs... were partially reprogrammed and re-expressed fibroblast genes. Most cells ended up taking the ‘dead-end... on Addgene.org Visit our stem cell page Find plasmids for reprogramming and differentiation Check out... -
Meet the New Addgene Board Members!
TypeBlog PostPublishedApril 28, 2020, 1:14 p.m....over 100 requests for the plasmids and the lab eventually deposited these plasmids at Addgene. While deposits...The mission to share critical reagents such as plasmids and viral stocks for researchers around the world... -
Viral Vectors 101: Transductions
TypeBlog PostPublishedApril 30, 2024, 1:15 p.m....typically transfected along with viral packaging plasmids into a viral production cell line, usually HEK...HEK-293 cells. When all of these viral plasmids are expressed within the production cell line, viral particles...targeted strategy, etc.) and episomal expression will last ~2-7 days at moderate levels. With regard to target... -
New and Upcoming Viral Vectors - September 2019
TypeBlog PostPublishedSept. 12, 2019, 1 p.m....is now complete! pAAV-CAG-GFP (plasmid 37825) and pAAV-hSyn-EGFP (plasmid 50465) are now available as 20...Here are some of the AAV we have released in the last few months. You can also browse our entire AAV inventory... -
Visualizing Genomic Loci with CRISPR-Sirius
TypeBlog PostPublishedApril 18, 2019, 12:53 p.m....Addgene.org Browse our CRISPR plasmids for visualization Find plasmids for your research Read our CRISPR..., give CRISPR-Sirius a try. Find CRISPR-Sirius plasmids at Addgene. References Ma, Hanhui et al. “Multicolor... -
Improving Transduction Efficiency with Exosome AAVs
TypeBlog PostPublishedMarch 14, 2019, 1:54 p.m....different cellular environments such as retina or glioblastoma). They also found that exo-AAVs were more resistant...Purifying exo-AAVs is actually a bit simpler than the classical purification protocol involving iodixanol gradients...brain tumor stromal cells in a mouse model of glioblastoma (Volak et al. 2018). The structure of exosome...