... give as a journal club to my molecular biology class. A Cell paper. With six figures. Averaging ten panels... assignment was clear: present a paper to our classmates so they could understand it. When my mind went...incidents above–and the dumpster noises were just last week!  Nerves It’s completely normal to be anxious...

... tag plasmids here! Where should I place the tag and how many do I need? When designing a plasmid containing...from Addgene More Resources on the Addgene blog Plasmids 101: Protein Tags Antibodies 101: ChIP Antibodies...

...   Cas12a – The staggered cutter  Originally classified as Cpf1, Cas12’s primary difference from Cas9.... Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system. Cell, 163(3): 759-771 (2015...More resources on the Addgene Website CRISPR Plasmids and Resources CRISPR Guide  ...

...Flash quiz! What pops into your head when you hear the phrase “action potential?” Firing neurons are ...through gap junction proteins which connect the cytoplasm of two cells) can also affect patterning and in...GEVI features increased brightness and improved plasma membrane specificity. Stable Step Function Opsin...

...to antibodies. Instead, they are derived from a class of binding proteins known as ankyrin repeat proteins...Resources Additional resources on the Addgene blog Plasmids 101: Aptamer FluorophoresAntibodies 101: scFVs...available from AddgeneBrowse our recombinant antibody plasmid collection References Grumezescu, A. M. (2018)....

...Chris Voigt for speaking with us! Find the Plasmids: Plasmids for multi-input CRISPR/Cas9 genetic circuits... required to get the function that you want. The last few years, we’ve really been making tons of parts...

... instead that it is noninvasive and has longer-lasting effects. Building your chemogenetic system To set...raising the threshold for signaling. The two main classes of receptors currently used in chemogenetics are...viral preps for your research Find chemogenetics plasmids for your research ...

...Originally published May 23, 2017 and last updated Jul 23, 2020 by Jennifer Tsang. CRISPR-Cas technology...CRISPR   Resources on Addgene.org Find CRISPR Plasmids for Your Research Interested in light-inducible...light-inducible Cas9 activity? Check out these these plasmids Catch Up on Your CRISPR Background with Our Guide Pages...

...the Quality Control team, I help make sure the plasmids that go out our doors are correct. Now that I...Shannon Rinaldi, Addgene’s Lab Manager, was taking classes at her local community college when she took her...interest in data science,” which she’s taking some classes on and hoping to pursue. The choice to not go to...

...drawbacks to this approach: TET proteins are not demethylases; they are dioxygenases that oxidize 5-methylcytosine...achieved by dCas9 alone, without TET (or any demethylase) fusion. This may seem counterintuitive: how ...achieved using lentiviral backbones expressing blasticidin or puromycin resistance genes.  It’s that simple...