We narrowed to 814 results for: LAS
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TypeBlog PostPublishedMarch 14, 2019, 1:54 p.m....different cellular environments such as retina or glioblastoma). They also found that exo-AAVs were more resistant...Purifying exo-AAVs is actually a bit simpler than the classical purification protocol involving iodixanol gradients...brain tumor stromal cells in a mouse model of glioblastoma (Volak et al. 2018). The structure of exosome...
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Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond
TypeBlog PostPublishedJune 1, 2021, 1:15 p.m.... to our volunteer list. We then collected and classified their responses, and used that information to... favors from becoming starting points for long-lasting partnerships Join us! We are a community of researchers...Resources on Addgene.org Browse Addgene's COVID-19 plasmids and resources ... -
Four Factors that Differentiate the Stem Cell Field
TypeBlog PostPublishedSept. 28, 2016, 2:35 p.m....Addgene Wesbite Find Reprogramming Plasmids Find Other Hot Plasmid Tools Visit Our Stem Cell Pages ...gratifying to see that Addgene has ensured that plasmid acquisition is not a rate-limiting step in stem... -
Management for Scientists: Delegating is Key
TypeBlog PostPublishedFeb. 20, 2015, 8:30 p.m....enough. For example, if we need to develop a new plasmid collection page, we certainly don't expect that...design every icon, curate all of the appropriate plasmids, and code the entire page. That would be entirely...Addgene Website Are You a Molecular Biologist? Find Plasmids for Your Research Here! ... -
How Addgene Creates Content
TypeBlog PostPublishedNov. 17, 2022, 2:15 p.m....that Addgene’s mission statement extends beyond plasmids, AAVs, and antibodies to our content, website,...mission is to enable that process. Our repository of plasmids, AAVs, antibodies, and data allows scientists ...Resources on the Addgene Blog Science careers Plasmids CRISPR Viral vectors Fluorescent proteins ... -
It’s Bioelectric! An Exciting Interdisciplinary Field of Research
TypeBlog PostPublishedOct. 29, 2019, 2:52 p.m....Flash quiz! What pops into your head when you hear the phrase “action potential?” Firing neurons are ...through gap junction proteins which connect the cytoplasm of two cells) can also affect patterning and in...GEVI features increased brightness and improved plasma membrane specificity. Stable Step Function Opsin... -
Transferable Skills Guide: Public Speaking
TypeBlog PostPublishedApril 20, 2023, 4 p.m.... give as a journal club to my molecular biology class. A Cell paper. With six figures. Averaging ten panels... assignment was clear: present a paper to our classmates so they could understand it. When my mind went...incidents above–and the dumpster noises were just last week! Nerves It’s completely normal to be anxious... -
To Each HIS Own
TypeBlog PostPublishedMarch 2, 2023, 2:15 p.m.... tag plasmids here! Where should I place the tag and how many do I need? When designing a plasmid containing...from Addgene More Resources on the Addgene blog Plasmids 101: Protein Tags Antibodies 101: ChIP Antibodies... -
CRISPR 101: Cas9 vs. The Other Cas(s)
TypeBlog PostPublishedDec. 8, 2022, 2:15 p.m.... Cas12a – The staggered cutter Originally classified as Cpf1, Cas12’s primary difference from Cas9.... Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system. Cell, 163(3): 759-771 (2015...More resources on the Addgene Website CRISPR Plasmids and Resources CRISPR Guide ... -
Antibodies 101: Affinity Reagents
TypeBlog PostPublishedApril 14, 2022, 1:15 p.m....to antibodies. Instead, they are derived from a class of binding proteins known as ankyrin repeat proteins...Resources Additional resources on the Addgene blog Plasmids 101: Aptamer FluorophoresAntibodies 101: scFVs...available from AddgeneBrowse our recombinant antibody plasmid collection References Grumezescu, A. M. (2018)....