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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 814 results for: LAS

Showing: 581 - 590 of 814 results

  1. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    Published
    Oct. 29, 2019, 2:52 p.m.
    ...Flash quiz! What pops into your head when you hear the phrase “action potential?” Firing neurons are ...through gap junction proteins which connect the cytoplasm of two cells) can also affect patterning and in...GEVI features increased brightness and improved plasma membrane specificity. Stable Step Function Opsin...

  3. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ... instead that it is noninvasive and has longer-lasting effects. Building your chemogenetic system To set...raising the threshold for signaling. The two main classes of receptors currently used in chemogenetics are...viral preps for your research Find chemogenetics plasmids for your research ...

  4. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    Published
    Dec. 14, 2021, 2:15 p.m.
    ...drawbacks to this approach: TET proteins are not demethylases; they are dioxygenases that oxidize 5-methylcytosine...achieved by dCas9 alone, without TET (or any demethylase) fusion. This may seem counterintuitive: how ...achieved using lentiviral backbones expressing blasticidin or puromycin resistance genes.  It’s that simple...

  5. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    Published
    July 23, 2020, 1:20 p.m.
    ...Originally published May 23, 2017 and last updated Jul 23, 2020 by Jennifer Tsang. CRISPR-Cas technology...CRISPR   Resources on Addgene.org Find CRISPR Plasmids for Your Research Interested in light-inducible...light-inducible Cas9 activity? Check out these these plasmids Catch Up on Your CRISPR Background with Our Guide Pages...

  6. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    Published
    June 25, 2020, 1:15 p.m.
    ...the Quality Control team, I help make sure the plasmids that go out our doors are correct. Now that I...Shannon Rinaldi, Addgene’s Lab Manager, was taking classes at her local community college when she took her...interest in data science,” which she’s taking some classes on and hoping to pursue. The choice to not go to...

  7. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...in analysis Plasmid-based proteins: plasmid sequence not accurate Check plasmid sequence for frameshifts...the full amount of time, every time. Imaging The last step in a western blot tends to be the most straightforward...

  8. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ...large-scale prime editing experiments come into play. Nicolas Mathis received his PhD from Gerald Schwank's lab...sciadv.aax9249 Koeppel, J., Weller, J., Peets, E. M., Pallaseni, A., Kuzmin, I., Raudvere, U., Peterson, H., Liberante....1038/s41587-022-01613-7 Mathis, N., Allam, A., Tálas, A., Kissling, L., Benvenuto, E., Schmidheini, L...

  9. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    Published
    Aug. 10, 2021, 1:15 p.m.
    ...Digital PCR for AAV Quantification Plasmid cloning by PCR  Plasmids 101: Colony PCR Site Directed Mutagenesis...amplicon can be used in molecular cloning to create a plasmid of interest, for detecting the presence of an insert...
Showing: 581 - 590 of 814 results