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...Vincent and Niroshi Senaratne, from the Biological and Biomedical Sciences program at Harvard University...and Genomics at Harvard Medical School, and is currently a fellow in clinical genetics at the University...the Program in Genetics and Genomics at Harvard Medical School. He studies developmental gene regulation...

...Niroshi Senaratne and Ben Vincent from the Biological and Biomedical Sciences program at Harvard University...and Genomics at Harvard Medical School, and is currently a fellow in clinical genetics at the University...the Program in Genetics and Genomics at Harvard Medical School. He studies developmental gene regulation...

...the sequence of interest. 5. Use a silica spin column for purification of the samples you send for DNA sequencing...requires you to perform your own Big Dye PCR amplification reaction (as opposed to using the all inclusive...Acetate/isopropanol precipitation method or using a silica spin column available from several vendors. The...image below), so I would strongly recommend using a silica spin column. They can be pricey, but well worth...

...reason for that. Lately, significant advances in the understanding and application of CRISPR/Cas9 technology... by even splashier news: Jiahao Sha of Nanjing Medical University had successfully used the CRISPR/Cas9...caused by genetic abnormalities, targeted genetic modification in monkeys is invaluable for the generation ... Times feature when he said, “All of this has basically happened in a year. It’s incredible.” Looking...

...for reference: Untergasser Lab Preparation of chemically competent cells (protocol) Preparation of electrocompetent...until we are ready to perform transformations. A typical 200mL bacterial culture produces 20mL of competent...protocols below: Untergasser Lab Transformation of chemically competent cells (protocol)  Transformation of...being performed at Addgene, this adds up to a significant time savings. You can also save yourself time...plasmid is introduced to bacteria, the bacteria is typically grown in liquid culture without antibiotics to...

...Scientists series will focus on an aspect of communication especially important for a manager – getting... Creating a culture of excellent, effective communication between all members of a team. As described ...what is going on and can contribute. Members periodically break, go exploring outside the team, and bring... Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn about her... opinions in meetings, via email, at formal communications events or at informal gatherings, like at lunch...

...you’ve saved. Repurposing regulated medical waste Regulated medical waste (RMV), which we collect in those...companies to obtain these labels! Reusing plastic Historically, scientists washed and reused materials such...flow cytometers. These fluidic machines handle biological samples and are cleaned between each use. You...those red biohazard bags, typically is destined for a landfill. However, Triumvirate Environmental developed...

...plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools more ... as these recent blog posts on prime editing, IgnaviCas9, and Nanoblades), but we hope that this blog ...engineered tRNA adenosine deaminase (TadA) to a catalytically impaired Streptococcus pyogenes Cas9. They called... toolkit named ORANGE (Open Resource for the Application of Neuronal Genome Editing) mediates targeted...(A-to-I) RNA editor called REPAIR by fusing catalytically inactivated RNA-targeting Cas13 (dCas13) with...

...problem is that scientists sometimes forget how to communicate about their work with non-academic people.” Daniel...while - a tool to expand perception, which is ironically what science is about. For me it is essential... time, defend the thesis, etc. Photography is critically important in many fields of biology and I believe...appreciated by a wider audience for more than just its biological importance. More of Daniel's stunning work can... new method to explore the inner structures of delicate plants and other organisms without destroying ...

...coming together, we can create a significant impact on both clinical care and public health for this current...match up scientists across fields with different technical knowledge that could help fight COVID-19. They...funding and volunteer opportunities, protocols, publications, and more to accelerate the pace of research...is based in Canada, much of the information is applicable to researchers worldwide. “COVID-19 has spread...