We narrowed to 714 results for: MAK

..., I’m picking two posts that describe methods to make single base edits in DNA. The first, contributed...a thorough walkthrough of how you can use PCR to make small sequence changes to plasmid DNA. This an oldie...CRISPR endonuclease (dCas9) fused to a deaminase to make the transitions at target sequences specified by...informative post. Interestingly, this technology may make some uses of Site Directed Mutagenesis by PCR obsolete...

... a job you wish you knew more about – how do you make a connection? An Informational Interview is a great...relationship building through Informational Interviews. Making Your informational interviews productive Informational...phone or video call. If you do decide to use Skype, make sure to follow the rules of successful, remote conversations...many good lists of questions on the internet. To make the most of the opportunity, use a written list ...

...Tip 1: Use color coding, labels, and post-its to make finding your most used protocols & references easy...for you) and place them on an easy to reach shelf. Make sure all notebooks are visibly labeled on the spine... tabs (for different experiments or projects) to make it easier to know where to start your search. Then...track the revision history. Now you’ll be able to make updates to protocols in real time. You may also ...

...contributed by our depositors, Addgene strives to make it easier for you to find the plasmids and publications...and the original depositor. Our citations feature makes it so that, when you cite a plasmid from Addgene...who may be working on similar projects and should make it easier for them to find your work later. We also...process. Special collections pages In an effort to make it easier for you to browse through the many plasmids...

...collection, the fluorescent proteins are, which makes mixing and matching a relatively easy proposition...Davidson lab for the last four years. “Researchers can make their own target if it is not already made for them...For more information on choosing a backbone and making fusions, see this blog post from Joachim Goedhart...Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection.  mEmerald plasmids and many...

... updated Sep 10, 2020 by Jennifer Tsang. CRISPR makes it easy to target multiple loci - a concept called...expressing multiple gRNAs on the same plasmid, you’ll make sure that each cell that gets the plasmid contains...increases the chance that all the edits you want to make will happen.  There are many reasons to multiplex...constructs with multiple promoter-gRNA cassettes, making them advantageous for small capacity vectors like... mammalian option described below, plasmids for making polycistronic gRNAs are also available from the...

... the absence of guide RNA, preventing Cas9 from making off-target cuts.   Pro tip! X-ray crystallography...base. In Figure 5, we can see why: two arginines make base-specific hydrogen bonds to the guanines, but.... Residues R1333 and R1335 from the CTD (yellow) make base-specific hydrogen bonds to guanines of the ...The HNH domain, which cuts the target DNA strand, makes few contacts with the rest of Cas9 and is highly...BioRender.com.   The exact conformational changes that make Cas9 such a valuable laboratory tool are still an...

...adopting a few meaningful changes in the lab could make a big difference in our global impact as scientists... environmental impact, but we can all do more to make research more environmentally friendly. Adopt one...Universities and companies have the potential to make a huge impact directly on waste, but also on culture...bring ideas to your workplace and you could end up making a huge impact on how waste is handled. A lot of...

...mapping These tools are for viewing, editing or making plasmid maps, but can also analyze and annotate...also has a suite of molecular biology tools and can make plasmid maps. Free for academic users. Serial Cloner... blank spaces, from the text of a DNA sequence, making it ready to copy and paste into other applications... Addgene blog: Learn how the CRISPR software matchmaker can help you choose the best CRISPR software ...

...video. Don't worry - no pipettes were harmed in the making of this photo!  Here, I was also given a mixture... understand the importance of using analytics to make decisions on content strategy. I really believe ...different best practices, create an email strategy, and make the occasional TikTok. I was also responsible for... my time here comes to an end, my worries about making connections with coworkers seem, in retrospect,...

...one of the most challenging [parts of my job], so making it a success feels like a real accomplishment.”... to take over hosting them. Since then, webinars make up a larger part of Hans’ workload. “I am currently....” A few other tips: “Learn HTML, write a lot to make sure you don’t hate it, and be flexible. The best...could be featured in future career blog posts. And make sure to subscribe to the Career Channel of the blog...

...medical use. Find More Optogenetic Tools Like pDawn  Making cassava plants resistant to cassava mosaic virus...this drought tolerant plant, Dr. Robertson aims to make use of the CRISPR/Cas9 technology to engineer plants...for example this yeast cloning kit) she intends to make yeast capable of desaturating fatty acids. Identification...Associate Professor (above, far right), thrives to make affordable biosensors that detect herbicides, such...

... in person to figure out ways we can make Addgene better and make scientists’ lives easier. Why transition... moved into this position because I want to help make the research enterprise work better and because ...learn ways Addgene can improve in its mission to make research easier. I’m hoping my story demonstrates...

...for imaging a protein’s entire life-cycle. This makes it nearly impossible for scientists to use GFP to...large, dim, and permanently attached to a protein, making them hard to amplify and image for long periods...in vivo. These probes however can be expensive to make, hard to deliver into living systems, and often ... proven to fold and function in living cells. To make these scaffolds specific to different targets, the...

...problems through good software design. But what makes software good? Fortunately, that question has been... task, we envision what the user wants to do and make the path to accomplishing their goals as intuitive...the most important controls front and center, and make specialized controls available when needed. SnapGene...For a coding sequence feature that may be used to make fusion genes, detection needs to occur even if one...

...you believe your idea has potential to make a difference, then make sure you’re able to answer every possible... Start with your network You've spent your life making connections and finding mentors -- well, it's time...discuss each project and business decision before making it, to have an internal review system.  On top ...

...method limits screening to a few hundred worms, making the detection of rare events difficult. Dickinson...resistance and the Roller movement phenotype. To make these markers easy to remove after injection, they... to generate a self-excising cassette (SEC). To make it possible fluorescently label proteins in C. elegans...fluorescent protein fusions, this system could be used to make many other genome modifications, including targeted...

..., as it can edit near to or far from PAM sites, making it less constrained by PAM availability than other...within the base editing window, while prime editors make a specific edit defined by the pegRNA. In cases ...the non-edited strand, they can cause the cell to remake that strand using the edited strand as the template... These variants are worth testing if you want to make your prime editing as efficient as possible. ...Some examples include: TwinPE: two prime editors make complementary edits to opposite strands of the DNA...

... strategies can make socially distanced work more creative. An example could be making lab meetings more...without leaving Zoom. The annotate function can also make the screen-sharing experience more interactive. ...: “Science is increasingly done in large teams, making it more likely that papers will be written by several...

...are able to make when they have access to shared materials, and then pay it forward by making the new materials...has been able to rapidly propagate this strain, making it available to scientists around the world. This...research by removing logistic hurdles to continue making materials available rapidly to scientists (Nature...

...pombe (fission) and S. cerevisiae (budding). Yeast make for an excellent model organism for a few reasons...phaffii is highly efficient at protein secretion, making harvesting recombinant proteins a cinch. This is...it doesn’t secrete its own endogenous proteins, making the recombinant protein its major secretion. Beyond... removing methanol from the media. This feature makes the strain tractable for peroxisomal studies on ...

...generating/replenishing kits, is to find new ways to make lab processes more efficient. Being in the Boston...there because both LabCentral and Addgene aim to make scientific advancement easier. NEBNext Ultra FS ...we did at the workshop (the kit is mostly used to make new libraries as opposed to replicating them like...new without spending a lot of time developing and making space for the techniques ourselves. As you probably...

...Christopher Voigt and Jeffrey Tabor have been working on making E. coli light responsive since 2005, when Tabor...systems continue to be optimized by Tabor’s lab, making light-controlled gene expression in E. coli easier...the response regulator encoded in the genome. To make the system less bulky and easier to use with other...Schmidl et al. chose to optimize these systems to make them more tunable and user-friendly. They experimented...

...the earliest) or a functional one. Pick one that makes sense for your skills, and the job you are applying...seconds on a resume when they first look at it. Don’t make them have to guess what you are capable of. You ...might know a thing or two about computer modeling. Make sure to focus on these skills. The only exception... yet for that dream job. Also, many universities make their job postings and salary expectations public...

... digest tips and tricks: The following tips will make it easier for you to obtain a useful and informative... recognition sequence. Most enzyme manufacturers make High Fidelity versions of the endonucleases and/...before loading them. The glycerol in the buffer will make sure your sample settles in the gel well and the...wrong! Quantify your DNA. Loading too much DNA will make it difficult to obtain crisp bands and analyze the...

...summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you...Parton Lab recently created a flexible system that makes use of pre-existing transgenic lines, termed BLITZ...system provides researchers better flexibility and makes the approach more accessible, as it reduces the ... be packaged into an AAV system. dCas9-SSAP will make the editing of kilobase-scaled sequences more efficient...

...character you painted in your personal statement. Make certain to choose your recommenders based on your...individuals are inundated with requests. The easier you make it for them, the likelier you will end up with a...lighting for a smooth performance. Most importantly, make certain that everyone you live with is aware of ...consistent with your personal statement, while you make the ultimate case: why you? 7. Strength in numbers...

...variety of educational resources in an effort to make scientific knowledge broadly available.  The ability... return the matching resources in an order that makes sense to the user. The devil is in the details of... of course: what does “best” mean? and what “makes sense” to a user? Moreover, there are many ways a user...institutions. There are many more technical details that make our new search engine unique. Most importantly, ...

...efficiency. Check out Addgene’s blog to learn about making your own competent cells and our protocols page... encodes a green fluorescent protein gene which makes the bacterium fluoresce green. Comic by Maya Kostman...Lytic phages hijack the bacterial hosts machinery to make more viral particles. Eventually the cell lyses ...bacterial chromosome and allows the donor bacterium to make a small “bridge” or sex pilus that attaches to the...

...feature, you can compare different Cas enzymes to make sure you are getting the desired features and cutting...and discover orthologs with similar properties (Makarova et al). CasPEDIA is an evolving database, and... to it! The CasPEDIA Consortium will, of course, make efforts to keep it updated with new entries and ...Structural Biology. https://doi.org/10.32942/X2C31F     Makarova, K. S., Wolf, Y. I., Iranzo, J., et al. (2019)...