... on various applications throughout my graduate career, and helped receive funding towards my PhD project...Empowers the writer, And offers insight into a career as a Professor. Step 1: Your PI forwards you...criteria, and award administration information. Carefully read over all parts until all aspects are understood...Grant Application" by Vid Mohan-Ram in Science Careers "Beginnings - How to write your first grant proposal...

...non-academic science careers Resources on Addgene.org: Sign up to receive Addgene’s Science Career Guide Check...rather than underdressed, but read emails from HR carefully because sometimes companies have relaxed dress...conferencing instructions you received from HR carefully. And even if you don’t have technical challenges...

...However, until recently safe delivery of nucleic acid cargo (DNA or RNA) to target cells has been challenging...serious adverse effects, and their immunogenic or carcinogenic profiles have limited their usability in clinical...It is an icosahedral, non-enveloped virus that carries a small, approximately 4.7 kb single stranded genome...single dose of subretinal administration of rAAV2 carrying the human gene RPE65, patients with clinical diagnosis...

... continue to shape the sector throughout their careers (McDowell et al, F1000Research, 2014; Dolan, F100Research...established, as they embark on or continue their careers in science-related fields. In particular, two recent... students in areas such as teaching salaries, healthcare access, and benefits; however, this may come ...Affairs at ASBMB Adam Fagen, PhD - Director, Early Career Scientist Segment at AAAS Marnie Gelbart, PhD -...

... deletion strains with overexpression plasmids carrying MET/Met genes from either S. pombe or S. cerevisiae...uracil, but contain methionine. (This allows cells carrying nonfunctional as well as functional MET genes ...only occur when galactose replaces glucose as the carbon source. Student results have shown that most, but...Basic Lab Techniques High School Students Explore Careers at Addgene Resources on Addgene.org Find Yeast...

...2010 Yuhai Cui Red mScarlet Split-wrmScarlet variant of mScarlet Split-wrmScarlet and split-sfGFP: tools...

..., to congenital disorders. A series of studies carried out on an isolated population in Sweden found that...that decreased their likelihood of death from cardiovascular disease but increased their risk of death from...paternal grandmothers early food supply influenced cardiovascular mortality of the female grandchildren." BMC...Gunnar, Lars O. Bygren, and Soren Edvinsson. "Cardiovascular and diabetes mortality determined by nutrition...

...www.FinishYourThesis.com and is one of many posts in our career series.  “The only healthy communication style ...into learning opportunities as you are advancing career, you will become the independent, assertive, and...learn more about assertiveness skills is Dale Carnegie’s book “How To Win Friends and Influence People...Management for Scientists" series More science career articles on our blog Read Joanne Kamen's Post ...

...their 30s who have quit their academic science careers in order to head to Ireland and take a chance as...product. It was a small project, not a full-blown career, but it was enough to start us down the entrepreneurial...started things up for real: bank account, business cards, incorporation, laboratory lease, etc. I still flip...allowed us to really analyze what we want out of our careers: to build something new and beautiful, to engage...

... homologous recombination events: The plasmid carrying the desired genetic modification integrates into...ShadowR is used as a FRET acceptor to mRuby2 or mScarlet. Image: Murakoshi et al., 2019. ShadowR is ... a FRET acceptor in combination with mRuby2 or mScarlet as the donor in HeLa cells.   Find ShadowR for... New Suite of Allelic-Exchange Vectors for the Scarless Modification of Proteobacterial Genomes. Applied...

...your LinkedIn and your resume and prepare business cards. You might not consider being a student or postdoc...postdoc a “business,” but having business cards is a good way to share your information quickly. During the...meet ten new people each day. Exchange business cards. When you’re done networking for the day (or whenever...have a small break), write down some notes on the card so you remember who they are and what you’ve talked...

...ICEBs1) system. ICEBs1 carries the genes needed for conjugation, but can also carry cargo genes, like Mobile-CRISPRi...from the labs of Jason Peters, Oren Rosenberg, and Carol Gross provides a streamlined suite of tools for ...

...how the cargo — in this case, the CRISPR components — are expressed. Unlike lentivirus, cargo delivered... vivo, in vitro, ex vivo CRISPR screens can be carried out using a few different methods — in vivo, in...outside a host cell’s genome. In this state, AAV cargo can be stably expressed in non-dividing cells for...

...to hold up your poster, and pack a few business cards to tack up next to your poster. Arrive early: You... presenter after the session. Exchange business cards or jot down their name and affiliation from the ...the innate immune system, and is interested in a career in science communication.       Resources on the...of the Addgene depositor community Learn about careers at Addgene ...

...While CRISPR is certainly not the first means to carry out so-called “forward genetic screening experiments...are your cells derived from? Currently, Addgene carries CRISPR libraries that target mouse, human, fly,...genetic modification are you trying to make? Addgene carries CRISPR libraries for gene knockout, activation,...or a specific class of genes? Addgene currently carries several genome-wide CRISPR libraries and a selection...

...non-dividing cells, lentiviral vectors can carry much more genetic cargo (8 kb vs 4.5 kb), and they remain popular..., and transfer plasmids. The packaging plasmid carried a mutated HIV-1 provirus that couldn’t package ...Kabbara N, Dalle B, Gourmel B, Socie G, Chrétien S, Cartier N, Aubourg P, Fischer A, Cornetta K, Galacteros...

...school district that has a lot of parents in STEM careers, chances are they’re wondering how they could contribute... led up to my job at Addgene, which is likely a career path none of them had imagined for a scientist....talked about CRISPR’s molecular mechanism, I was careful to keep it relatively simple. I honed the complexity...testing your SciComm skills or are interested in a career in education. Maybe you just like to see the look...

..., each package is opened and the contents are carefully inspected to verify that the samples arrived intact...dedicated and detail-oriented lab team that works carefully to catch mistakes. Each sample that is transformed...shared with multiple people or even multiple labs. Careful tracking prevents us Addgenies from wasting hours... time searching for samples and keeps us from discarding tubes in error. The Addgene lab works diligently...

...scheme very nice at first sight in that Addgene takes care of everything including MTA and shipping. Addgene... scientific societies. Addgene: Throughout your career, you have held research appointments in the US ...different parts of the world in my early research career. I've found that research institutes in different... Caroline LaManna...

...students, rotation students, joining a new lab at any career stage, learning a new technique while visiting ...rightfully so! They can provide you with high-level career advice, open doors for you, and teach you valuable...identify a ‘scientific mentor’: someone who provides career advice and offers direction on your scientific ...hasn’t sent you papers or protocols yet, don’t be scared to ask for some! You can email or ask in-person...

...Read all of our career blog posts Resources on Addgene.org Learn more about careers at Addgene Watch...engaging your audience. You need to make your audience care, to want to know the answer to your research problem...Watch our career videos ...

...and Sota Hiraga found that if a mini-F plasmid carried a specific 700 bp segment of DNA, the plasmid was... without plasmid addiction systems. For example, Cara Morin and James Kaper used the hok/sok system to...be used over prolonged periods. Using a plasmid carrying the hok/sok toxin-antitoxin system, they were ...223.1 (1992): 41-54. PubMed PMID: 1370544. Morin, Cara E., and James B. Kaper. "Use of stabilized luciferase-expressing...

...platform to deliver various cargo to a specific DNA locus by directly fusing the cargo to dCas9.  This technique...next? Designing or selecting a sgRNA Addgene may carry a validated sgRNA for your target gene.  Validated...CRISPR Experiment Cas9 expression systems Addgene carries a variety of Cas9 containing plasmids that have...

...Non-Academic Career Decisions Check Out Our Management for Scientists Series Science Career Options Resources...Addgene.org Learn more About Careers at Addgene ... academic career...

..., if you know what you need. Most often, early career scientists aren’t trained to have these types of... and not on the financial aspects. Money often carries an emotional weight. Therefore, too much focus ... a pathology microtome/cryostat, then making a careful decision on the condition and availability of the...XpDesignsMGNT        Additional Resources Find other career posts on the Addgene Blog! Learn more about Experimental...

...You've worked hard designing your plasmid – you carefully selected the optimal promoter for your gene of...recognize your little prokaryotic minions that carried out the labor-intensive process of replicating ...is appropriate for propogating your plasmid or carrying out your experiment. History of E. coli strains...metabolism pathway Inability to utilize arabinose as a carbon source leuB β-isopropyl malate dehydrogenase ...

...the example at right using blue flame plasmid pmScarlet_C1! After creating the T7-DNA template, you'd use...right homology arm to the desired insert (e.g., mScarlet).   Plasmid ID Plasmid Name Tags ...Yang, Hui, et al. "One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas-mediated... robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA...

...and we both won accolades that would launch our careers in science. Fast forward nearly 20 years and ... single author? I’ve seen so few in my 20+ year career that I can’t help but feel a bit dubious when I...Resources on Addgene.org Check out Career's at Addgene ... career...

...laboratory. The risk of generating RCL is low but extra care has to be taken especially if you are working with...of interest.   Advantages Disadvantages Can carry large transgenes (>8 kb) Potential for generation...feature for your experiment; it must be chosen carefully. Often researchers want a simple set-up where ...oncogenes for instance should be manipulated with care. See the NIH’s biosafety guidelines for more information...

...2007): 1740-1743. PubMed PMID: 17882145. 2. Zincarelli, Carmela, et al. "Analysis of AAV serotypes 1–9 mediated...make AAVs useful for a wide variety of experiments, care still needs to be taken when selecting which particular...22418061. PubMed Central PMCID: PMC3381869. 7. McCarty, D. M., Paul E. Monahan, and Richard J. Samulski...