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Showing: 151 - 180 of 262 results

  1. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ...Hackerspace Website https://wiki.hackerspaces.org/Hackerspaces OpenWorm Project Website: http://www.openworm.org...www.openworm.org/ Cat Microbiome Project Website: http://www.kittybiome.com/   ...

  2. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    Published
    July 2, 2020, 12:40 p.m.
    ...organization deciphering. Molecular Cytogenetics, 11. https://doi.org/10.1186/s13039-018-0368-2  Liu X, Zhang...by Biotinylated dCas9. Cell 170:1028–1043.e19 . https://doi.org/10.1016/j.cell.2017.08.003 Liu, X., Chen...chromatin by biotinylated dCas9. Genome Biology, 21. https://doi.org/10.1186/s13059-020-01973-w Additional ...

  3. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    Published
    Sept. 12, 2017, 1:44 p.m.
    ...Vector Delivery. Molecular Therapy 12:744–753 . https://doi.org/10.1016/j.ymthe.2005.06.003 Guerin K, ...Viral DNA Extracts. Human Gene Therapy 31:664–678 . https://doi.org/10.1089/hum.2019.277 Lecomte E, Tournaire...Sequencing. Molecular Therapy - Nucleic Acids 4:e260 . https://doi.org/10.1038/mtna.2015.32 Wright JF, Zelenaia...

  4. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    Published
    Oct. 10, 2023, 1 p.m.
    ...Journal of Clinical Microbiology, 23(5), 959–961. https://doi.org/10.1128/jcm.23.5.959-961.1986 Lobstein...its cytoplasm. Microbial Cell Factories, 11, 56. https://doi.org/10.1186/1475-2859-11-56 Trastoy, M. O....cleavage in human cells. Mutagenesis, 20(2), 111–114. https://doi.org/10.1093/mutage/gei016I Resources on Addgene.org...

  5. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ...viral vector production. Neuromethods, 195, 77–101. https://doi.org/10.1007/978-1-0716-2918-5_5 Haery, L.,...Neuronal Manipulation. Frontiers in Neuroanatomy, 13. https://www.frontiersin.org/articles/10.3389/fnana.2019.00093...Protocols in Molecular Biology, 115, 31.5.1-31.5.13. https://doi.org/10.1002/cpmb.8...

  6. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ...CRISPR-Cas9 System. Appl Environ Microbiol 81:2506–2514 . https://doi.org/10.1128/aem.04023-14 Li Q, Sun B, Chen...Escherichia coli. Acta Biochimica et Biophysica Sinica. https://doi.org/10.1093/abbs/gmab036 Zhang Y, Sun X, Wang...CRISPR-Associated Transposases. ACS Synth Biol 9:1998–2008. https://doi.org/10.1021/acssynbio.0c00073   Additional...

  7. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    Published
    April 8, 2025, 1:15 p.m.
    ...Journal of Neuroscience Methods, 172(2), 250–254. https://doi.org/10.1016/j.jneumeth.2008.05.003 Ghosh, ...blots. Expert Review of Proteomics, 11(5), 549–560. https://doi.org/10.1586/14789450.2014.939635 Gilda, J....blots. Analytical Biochemistry, 440(2), 186–188. https://doi.org/10.1016/j.ab.2013.05.027 Additional Resources...

  8. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    Published
    Nov. 16, 2021, 2:15 p.m.
    ...Molecular Biology. Springer New York, pp 299–311. https://doi.org/10.1007/978-1-4939-8935-5_26 Stuurman ...Introduction to Fluorescence Microscopy. iBiology. https://www.ibiology.org/talks/fluorescence-microscopy...validation of antibodies. Nat Methods 13:823–827. https://doi.org/10.1038/nmeth.3995 Additional Resources...

  9. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ... Tet-On and Tet-Off systems: Second generation. https://www.takarabio.com/products/gene-function/tet-inducible-expression-systems...in mammalian cells. BMC Biotechnol 6, 43 (2006). https://doi.org/10.1186/1472-6750-6-43Kaczmarczyk A, Vorholt...overexpression transgenes. Sci Rep 12, 3835 (2022). https://doi.org/10.1038/s41598-022-07852-7 De Boer, Herman...

  10. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    Published
    Oct. 8, 2020, 1:15 p.m.
    ...Mammalian Cell Culture. Anal Chem 84:4227–4232 . https://doi.org/10.1021/ac2033112 Ligasová A, Vydržalová...Mycoplasmas Using Fluorescence Microscopy. Cells 8:1510 . https://doi.org/10.3390/cells8121510 Sung H, Kang SH, ...Cell Lines. In Vitro Cell Dev Biol Anim 38:86 . https://doi.org/10.1290/1071-2690(2002)038<0086:caeomi...

  11. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    .... Review of Scientific Instruments 43:404–409 . https://doi.org/10.1063/1.1685647 McKinnon KM (2018) Flow...Overview. Current Protocols in Immunology 120: . https://doi.org/10.1002/cpim.40 Picot J, Guerin CL, Le...recent instrumentation. Cytotechnology 64:109–130 . https://doi.org/10.1007/s10616-011-9415-0 Additional resources...

  12. Trends in CRISPR and SynBio Technologies

    Type
    Blog Post
    Published
    Feb. 4, 2015, 3:58 p.m.
    ...of online user forums such as http://www.genome-engineering.org and http://www.egenome.org." At the Keystone...

  13. Congratulations, Deck The Lab winners!

    Type
    Blog Post
    Published
    Jan. 14, 2021, 1:30 p.m.
    ... #WinterWonderlab @Addgene Make your own here!: https://t.co/AnltWfKuQg pic.twitter.com/OOrwo9feyJ — BTC...Remind them you care!#thanks #health #mentalhealth https://t.co/Td2M6AOpuW pic.twitter.com/ofLI4eD8Zs — Microbigals...

  14. Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Feb. 25, 2025, 2:15 p.m.
    ...for antibody characterization. Nature Protocols. https://doi.org/10.1038/s41596-024-01095-8 Kahn, R. A....reproducibility in biomedical research. eLife, 13, e100211. https://doi.org/10.7554/eLife.100211...

  15. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

    Type
    Blog Post
    Published
    June 8, 2023, 1:15 p.m.
    ...diverse tissues. Nature Protocols, 17(2), Article 2. https://doi.org/10.1038/s41596-021-00644-9 Radtke, A. ...National Academy of Sciences, 117(52), 33455–33465. https://doi.org/10.1073/pnas.2018488117 More resources...

  17. Antibodies 101: Affinity Tags

    Type
    Blog Post
    Published
    Sept. 19, 2023, 1 p.m.
    ...Protocols in Protein Science. John Wiley & Sons, Inc. https://doi.org/10.1002/0471140864 Fox, J. D., & Waugh...Protocols (Vol. 205, pp. 99–118). Humana Press. https://doi.org/10.1385/1-59259-301-1:99 Resources on ...

  18. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    Published
    May 2, 2023, 1 p.m.
    ...eukaryotic proteome? Open Biol. (2019) 9:180241. DOI: https://doi.org/10.1080/rsob.180241 Vanni, C., et al.,...coding sequence space. eLife. (2022) 11:e67667. DOI: https://doi.org/10.7554/eLife.67667 Springer, S. P. Gene...

  19. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ... human disease.     Sun, et al. bioRxiv. 2021. https://doi.org/10.1101/2020.05.04.076067    Novel fluorescent...Chesterfield et al., ACS Synthetic Biology 2020. https://pubs.acs.org/doi/10.1021/acssynbio.0c00192   ...

  20. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    Published
    June 11, 2020, 1:34 p.m.
    ...Review of Pharmacology and Toxicology 55:399–417 . https://doi.org/10.1146/annurev-pharmtox-010814-124803...Chemogenetics. In Methods in Enzymology (pp. 181–196). https://doi.org/10.1016/bs.mie.2018.01.022 Additional ...

  21. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    Published
    Dec. 14, 2020, 2:15 p.m.
    ... GPCR transducerome. Nat Chem Biol 16:841–849 . https://doi.org/10.1038/s41589-020-0535-8 Takeda S, Kadowaki...human genome sequence. FEBS Letters 520:97–101 . https://doi.org/10.1016/s0014-5793(02)02775-8 Additional...

  22. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    Published
    April 25, 2023, 1:15 p.m.
    ...The Immunoassay Handbook (pp. 245–265). Elsevier. https://doi.org/10.1016/b978-0-08-097037-0.00017-8     .... 269, Issue 17, pp. 13048–13055). Elsevier BV. https://doi.org/10.1016/s0021-9258(18)99982-5 More resources...

  23. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ...      Moore et al., ACS Synthetic Biology 202. https://doi.org/10.1021/acssynbio.0c00581   New tool ...Brookhouser, N., et al. BMC Biol 18, 193 (2020). https://doi.org/10.1186/s12915-020-00929-7    The CRISPR...

  24. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    Published
    Aug. 11, 2020, 1:15 p.m.
    ...Targeted Neuronal Manipulation. Front Neuroanat 13: . https://doi.org/10.3389/fnana.2019.00093 Wilmott P, Lisowski... Virus. Human Gene Therapy Methods 30:206–213 . https://doi.org/10.1089/hgtb.2019.276 Additional resources...

  25. Addgene Depositors Get More Citations

    Type
    Blog Post
    Published
    April 20, 2017, 2:30 p.m.
    ...research. View her research on her SSRN Author page: https://ssrn.com/author=1232466.     Neil Thompson is ...control trials in innovation. Visit his website at: http://www.neil-t.com/ .   References 1. Cong, Le, et...

  27. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    Published
    Dec. 18, 2014, 4:06 p.m.
    ...Relations Director for Curiosity Collider (hyperlink https://curiositycollider.org/) , which she co-founded...Outreach Manager for Science Borealis (hyperlink http://scienceborealis.ca/)    ...

  28. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    Published
    Jan. 25, 2024, 2:15 p.m.
    ...Biochemistry, Biophysics, and Structural Biology. https://doi.org/10.32942/X2C31F     Makarova, K. S., Wolf...variants. Nature Reviews Microbiology, 18(2), 67–83. https://doi.org/10.1038/s41579-019-0299-x Resources on...

  29. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...vaccine development. Vaccines. 7, 75 (2022). DOI: https://doi.org/10.1038/s41541-022-00503-y Gorry PR, ...landscape. Sig Transduct Target Ther 6, 53 (2021). DOI: https://doi.org/10.1038/s41392-021-00487-6 Additional ...

  30. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    Published
    Feb. 28, 2023, 2:15 p.m.
    ...microscopic imaging. Nat Commun 11, 5979 (2020). https://doi.org/10.1038/s41467-020-19447-9 Hohlbein, J...: quo vadis?. Nat Methods 19, 1020–1025 (2022). https://doi.org/10.1038/s41592-022-01602-3...
Showing: 151 - 180 of 262 results