960 results

An exploration of how the Trimmer lab developed their Addgene-deposited collection of recombinant antibodies.

April 5, 2022, 1:20 p.m.

A description and video demonstration of the new FLIES plasmid.

April 1, 2022, 1:15 p.m.

Addgene is announcing the launch of their new ready-to-use recombinant antibody collection.

March 28, 2022, 4:30 p.m.

Antibody validation shows that your antibody is targeting exactly what it is supposed to. This post walks through 5 validation strategies.

March 24, 2022, 1:15 p.m.

We guide you through a series of questions and considerations to help you pick an antibody for any experiment, along with a handy flow chart.

March 15, 2022, 1:15 p.m.

A basic overview of chromatic immunoprecipitation (ChIP)

March 10, 2022, 3:25 p.m.

Learn how to identify negotiation skills you already use in your lab and life and develop them further as a professional skill set.

March 3, 2022, 2 p.m.

RUBY-red is a new plant marker that uses a bright red color for easy identification of transgenic plants.

March 1, 2022, 2:15 p.m.

Help us name our new antibody mascot!

Feb. 24, 2022, 2:15 p.m.

Our February hot plasmids, including two new CRISPR tools, a protein ladder, and anti-FLAG frankenbodies.

Feb. 22, 2022, 2:15 p.m.

Two new CRISPR tools from the Abudayyeh-Gootenberg lab are now available at Addgene: Cas7/11 and PASTE.

Feb. 17, 2022, 2 p.m.

A guide to help you improve focus and reduce mistakes when at the bench.

Feb. 8, 2022, 2:15 p.m.

A quick overview of antibody storage, buffers, and conjugates.

Feb. 1, 2022, 2:15 p.m.

We announce the winners of our 2021 Deck the Lab competition!

Jan. 25, 2022, 2:15 p.m.

Learn about how to identify and develop creativity in the lab - a skill that's valuable in and out of the sciences!

Jan. 18, 2022, 2:15 p.m.

Multiple antibodies often exist for a single target protein, allowing for the targeting of different epitopes as protein conformation changes.

Jan. 11, 2022, 2 p.m.

An introduction to conflict resolution, identifying types of conflict and strategies for solving them.

Jan. 4, 2022, 2:15 p.m.

An end of year list of 12 CRISPR resources available on Addgene, focusing on ones added in 2021.

Dec. 28, 2021, 2:15 p.m.

An overview of secondary antibodies and the indirect approach in immunoimaging assays.

Dec. 21, 2021, 2:15 p.m.

An easy demethylation protocol uses CRISPR-based dCas9-directed DNA avoids the use of Tet

Dec. 14, 2021, 2:15 p.m.

An introduction to the use of antibodies in immunoprecipitation, including antibody immobilization, controls, and a basic overview of techniques.

Dec. 7, 2021, 2:15 p.m.

Get into the Holiday Spirit with Addgene's annual #DeckTheLab contest!

Dec. 2, 2021, 2:15 p.m.

In this post from our Transferable Skills series, learn how problem solving is a core part of your research training as well as a valuable skill for your future career.

Nov. 30, 2021, 2:15 p.m.

Learn about the latest CRISPR plasmids deposited at Addgene, including a miniature CRISPR-Cas systems, SEND plasmids, and prime editing improvements

Nov. 18, 2021, 2:15 p.m.

Introduction to immunofluorescence and how you can use it to illuminate your research.

Nov. 16, 2021, 2:15 p.m.

Learn about the sources of spontaneous recombination and optimizing Cre-recombinase dependent AAV vector expression in vivo.

Nov. 2, 2021, 1:15 p.m.

An antibody’s isotype is determined by its heavy-chain constant region and influences what role the antibody plays in an immune response.

Oct. 19, 2021, 1:15 p.m.

Since several sets of near-infrared fluorescent proteins (NIR FPs) and biosensors have been created recently, we've added a quick guide to help you choose the best probe for your research needs.

Oct. 5, 2021, 1:15 p.m.

Recent highlights from Addgene's collection include a system to examine biosynthetic pathways, and a new tool for enriching ABE-targeted cells.

Sept. 21, 2021, 1:15 p.m.

Having trouble with viral vectors? Here are several tips to help prevent recombination in viral vectors or other plasmids with repeating elements.

Sept. 14, 2021, 1:15 p.m.

Announcing Addgene's new Intro to the Lab Bench, a collection of protocols, videos, and quizzes to help guide those new to working in the lab.

Sept. 7, 2021, 1:15 p.m.

ELISA are used to quantitatively detect protein concentrations in experimental samples. Learn about different types of ELISA and when to choose each method.

Aug. 24, 2021, 1:15 p.m.

Get a preview of new science-themed GIFs from Addgene and learn how to find them on Twitter

Aug. 17, 2021, 1:15 p.m.

The basics of PCR covering end point PCR, qPCR, ddPCR, and multiplex PCR and how each method is used.

Aug. 10, 2021, 1:15 p.m.

See the winners for the 2021 Michael Davidson and Roger Tsien Commemorative Conference Award.

Aug. 5, 2021, 12:15 p.m.

Meet Addgene's Newest Board Member Bart Newland.

Aug. 3, 2021, 1:15 p.m.

Recent highlights from Addgene's collection include CRISPR-based editing in organoids and Biosensors for detecting strigolactones.

July 27, 2021, 1:15 p.m.

Get an overview of flow cytometry including how it works, what you can use flow cytometry for, and tips and tricks for a successful flow cytometry run.

July 20, 2021, 1:15 p.m.

Polyclonal antibodies are a common research reagent - learn how they’re produced and the advantages and disadvantages of using them in your next experiment.

July 14, 2021, 1:15 p.m.

Find plasmid tools for COVID-19 research expressing viral open reading frames, host factors, and tools for detecting SARS-CoV-2 and generating pseudoviruses.

July 12, 2021, 1:15 p.m.

An introduction to transposons including Sleeping Beauty, piggyBac, and Tol2 and how they can be used in transposon mutagenesis screens, gene transfer, and creating transgenic animals.

July 6, 2021, 1:28 p.m.

Get the basics on monoclonal antibodies including how monoclonal antibodies are made and why use monoclonal antibodies for your experiment.

June 29, 2021, 1:15 p.m.

Plasmids from Addgene encoding SARS-CoV-2 spike protein variants can be used to study antibody neutralization escape.

June 22, 2021, 1:15 p.m.

Use blue light to control gene expression from PBAD promoters. It's more spatially and temporally precise compared to AraC based expression vectors.

June 17, 2021, 1:17 p.m.

What is research? How do I find a lab? How should I approach research after finding a lab? Get answers to these questions and more about undergrad research.

June 8, 2021, 1:15 p.m.

This guide on single chain fragment variables (ScFvs) covers how ScFvs are made, the advantages and disadvantages of using ScFvs, and several applications.

June 3, 2021, 1:15 p.m.

Hear from Crowdfight members to see how their platform got started during COVID-19 to help connect scientific research and learn how they're expanding!

June 1, 2021, 1:15 p.m.

With this NIH's BRAIN Initiative Grant, Addgene will provide ready-to-use recombinant antibodies and will build a crowdsourced antibody Data Hub.

May 25, 2021, 2:29 p.m.

Get a behind-the-scenes look at how we've adapted video production during COVID-19.

May 20, 2021, 1:15 p.m.

Recent highlights from Addgene's collection include tools to study SARS-CoV-2 antibody escape, antibodies for aminoacyl tRNA synthetases, and plasmids for generating nanocompartments for yeast bioproduction.

May 18, 2021, 1:15 p.m.