We narrowed to 868 results for: REN

...than 300 different coactivator proteins individually contribute to cell regulation in a coherent manner.... one of the most successful targets for drugs currently available or being developed to treat a multitude...patterns of nuclear receptors across multiple different tissues in normal and disease states, their circadian...previously uncharacterized cellular roles. In the current phase of NURSA we are seeking to compliment these...our data mining tool, Transcriptomine. We are currently nearing the completion of an 18-month effort to...found in the Datasets and Reagents sections of the current NURSA website. NURSA and Addgene The great progress...

...life-threatening consequences. They result from defects in different steps in the glycan biosynthetic pathway and have...characterized by a predisposition to severe and recurrent infections along with a mild or exacerbated inflammatory...infection and other pathological conditions. References: Monticelli, M., Ferro, T., Jaeken, J., dos Reis...multi-subunit macromolecular complexes, but a serendipitous discovery led them to investigate the use of... discovered that the in vitro functions of 11 different Arg-mutant enzymes could be rescued by supplementation...diseases caused by Arg-mutant proteins, but there is currently no evidence of Arg-mutant protein rescue in living...from rare diseases arising from Arg mutations. References: Baldwin, Enoch, et al. "Generation of ligand...

... 1-4 different gRNAs. Viral envelope proteins To alter the cell tropism of Nanoblades, different viral...incubating Nanoblade particles with polybrene, a cationic polymer. Polybrene has long been used to increase...electroporation, and viral transduction; but these methods aren’t stealthy enough to gain access to some cells and...embryos, and animals. Nanoblades can also carry different types of CRISPR cargo including RNPs for indel...versatile and accessible genome editing tool.   References Mangeot, Philippe E., et al. "Genome editing ...

...job hunt, I quickly noticed one of the biggest differences between applying for an academic position versus... phone interview. I also transitioned between different lab groups without having a formal interview process...usually have a theme too, which is something not inherently captured by the STAR method. I like to think ... try and circumvent this, test out the video conferencing software you’ll be using in advance of the interview...interview, if possible. Otherwise, read the video conferencing instructions you received from HR carefully....completing each section of your talk. Some video conferencing software allows participants to “raise” their... wouldn’t have made it to the interview if you weren’t qualified for the position. So prepare for the ...

...remember: Keep Ca+2lm and Ca+2rry on! References and Resources References Nasu Y, Shen Y, Kramer L, Campbell...sensors are commonly used calcium reporters with different kinetic parameters. There are three GCaMP types...and Localization The calcium concentrations in different subcellular compartments can vary greatly. Additionally...measurements are more susceptible to artifacts and pH interference, and comparison between experiments can be more...difficult due to variance in sensor expression if you aren’t working with a stably integrated biosensor. Ratiometric... also suitable for in vivo studies. There are different versions optimized for neuron-specific, astrocyte-specific...

...never been so important.     References and resources References Biering SB,  Sarnik SA, Wang E, Zengel...for generating pseudovirus and those encoding different spike protein variants that can be incorporated...vaccinated individuals are at neutralizing the different SARS-CoV-2 spike variants. You can learn more ...head over to this blog post that highlights five different methods to use CRISPR to detect SARS-CoV-2. Other... including those from Andrea Pauli and Julius Brennecke. They developed a method that uses loop-mediated...Whiteman MB, McKay LGA, Lelis FJN, Habibi S, Cai Y, Rennick LJ, Duprex WP, McCarthy KR, Lavine CL, Zuo T, Lin...

...their current promoters, as well as more promoters that target cells types missed by their current library... Clinical Ophthalmology Basel recently took a different approach to enhance specificity in retinal cells... synthetic promoters, the Roska lab used four different design strategies: Strategy #1 (ProA): Create... this library contained 226 AAVs, each with a different synthetic promoter driving expression of the same...was from a single class of cells  Of the four different promoter designs, promoters from strategy #4 had...ready-to-use AAV viral preps Browse all viral vectors References Juettner, J., Szabó, A., Gross-Scherf, B., Morikawa...

...into the margins of my protocol. While these tips weren’t critical to the experiment, they definitely made... AAV packaging plasmids. The cells need three different plasmids to produce AAV: 1) the RepCap plasmid...gradient ultracentrifugation uses a gradient of different concentrations of iodixanol to separate out contaminants...titer generated by qPCR is useful, I test a few different concentrations of virus, or multiplicity of infections...variability. Check out this post to learn more about different ways to titer AAV. Do you have any AAV production...teaching me about AAV production and purification! References 1. Aurnhammer, C., Haase, M., Muether, N., Hausl...adeno-associated viral vectors for in vitro and in vivo use. Current protocols in molecular biology, Chapter 16, Unit...

...test:  my undergrads were different people! More specifically, they had different learning styles. One was... can be very different from what motivates you!), know their unique skills and strengths (you will likely...likely have reports with different levels of experience), find out what their learning styles are and capitalize... to you to decide which one you want to be!  References Becoming the boss. Harvard Business Review, ... Karen Guerin...

...but timer activity can be calibrated to these different conditions, enabling the use of a given timer ...While Tsuboi et al developed mK-GO somewhat serendipitously while attempting to enhance mKO in other ways... of Neurog3 (a gene controlling pancreatic differentiation) in mouse embryos (Miyatsuka et al.) Distinguish... Let us know in the comments section below! References 1. Terskikh, Alexey, et al. ""Fluorescent timer...11090358. 2. Tsuboi, Takashi, et al. "Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine...and Michael S. German. "Chronology of islet differentiation revealed by temporal cell labeling." Diabetes...programmable synthetic lineage-control network that differentiates human IPSCs into glucose-sensitive insulin-...

...have proposed the concurrent use of “orthogonal” Cas9 proteins derived from different bacterial species...variants—nuclease-competent or nuclease-null—in order to effect different perturbations. Consider, for instance, the challenge... both cutting and regulation of expression at different genetic loci within a single cell. One might imagine...expression (6). Altering the length of gRNAs targeting different sites of interest thus represents a straightforward...introduced together with truncated gRNAs targeting a different set of loci to induce simultaneous cutting and...DNA sequence or regulating gene expression.     References 1. Esvelt, Kevin M., et al. "Orthogonal Cas9 ...

...safely and transparently.  For more information, visit www.sculptingevolution.org. References: Burt, ...didn't work quite as well as anticipated4, greater awareness will certainly improve safety. What safeguards...vectors of diseases such as malaria and dengue are currently being performed in regions that don't have resident...Cas9 genome editing. Finally, gene drives are an inherently collective technology: because they necessarily...dengue for many years. Since more than a thousand children died of malaria today, let's not risk a potential...information about gene drives can be found in the referenced publications. Thank you to our guest blogger...

... mutagenesis or gene targeting Victor de Lorenzo's lab has engineered a modular mini-Tn5 vector that... microscope 8 hours post-pulse. The distinct differences of red-to-green fluorescence in individual mitochondria...mitochondria in the networks marks the differences in import of newly synthesized (green) protein and ...five previously unknown channelrhodopsins from different species: Stigleoclonium helveticum (ShChR), Chlamydomonas...cassette. They validated this methodology in two differents types of bacteria (E. coli and Salmonella typhimurium... of all four final RVD modules in each of two different TALE scaffolds are provided, as the optimal scaffold...region of one neuron and postsynaptic region of a different neuron, come into proximity in the synaptic cleft...

...delivered on a different plasmid and allows simultaneous multiplex editing of six different genes. This ...flanking the promoter-gRNA construct, but with different sequences adjacent to the sites. When digested...assembly principles, but they’re optimized for different purposes.   Gersbach Lab multiplexing plasmids...kanamycin-resistant plasmids, each containing a different gRNA target sequence downstream of the 7SK, human...users to express up to 7 gRNAs. The kit contains different destination vectors depending on the total number...transcript. Rather than being transcribed from different promoters, the gRNAs are transcribed together ...likely be used in a wide range of bacteria. References 1. Maddalo, Danilo, et al. “In vivo engineering...

...It was by serendipity that I got into the field of gene therapy, more specifically AAV-based retinal ...treatment, famously visited Congress to help increase awareness about the potential of gene therapy!). It would...is available. While only natural serotypes are currently used in clinical trials, a variety of engineered... AAV safety profile is excellent and further strengthens its position as the vector of choice for in vivo...and the development of personalized medicine. References and further reading 1. FDA approves gene therapy...the Light after 25 Years of Retinal Gene Therapy. Trends Mol Med. 2018, S1471-4914(18)30123-0. DOI: 10.1016... Karen Guerin...

...be identified by PCR later. The PNS presents a different problem: it’s spread throughout the body and is..., AAV.PHP.eB was used at a lower dose than it’s parent AAV.PHP.B: 1x1011 vs 1x1012 viral genomes (vg) ...comparison of AAV.PHP.eB and AAV.PHP.S to their parental vectors. To quantify this comparison, capsids ...efficiency of AAV-PHP.eB and AAV-PHP.S versus their parental AAV vectors. What can these AAV’s be used for...   Cortex Striatum Cerebellum AAV.PHP.B (parental) 49 +/- 7% 36 +/- 8% 37 +/- 10% AAV.PHP.eB 69...Transduced   Dorsal Root Ganglion (DRG) AAV.9 (parental) 46 +/- 0.7% AAV.PHP.S 82 +/- 2% Table 2:... and supplemental figure 8 in Chan et al).  References 1. Chan, KY, Jang MJ, Yoo BB, et al. Engineered...

...space to be oxidized and form disulfide bonds (Frenzel et. al., 2013). If you’re using a mammalian production...: The steps of rAb production. Created with biorender.com   Another method of harvesting uses magnetic...useful to compare recombinant antibodies to their parental hybridomas. Aggregation and storage Aggregation...our Antibodies 101 blog series.  Resources and references More resources on the Addgene blog Introduction...Purification Protocol Addgene's Antibody Guide References Trimmer JS. Recombinant Antibodies in Basic Neuroscience...: 10.1007/978-1-4939-8730-6_1. PMID: 30242676. Frenzel A, Hust M, Schirrmann T. Expression of recombinant...particle formation, characterization & rheology, Current Opinion in Colloid & Interface Science, Volume ...

...Testing of three different AAV serotypes (AAV1, 8, 9) injected into the A2 noradrenergic area of the caudal...helping everybody, helps everybody! References and resources References Dayton, R. D., Grames, M. S., & ...scientists under-informed with respect to how different serotypes, promoters, and injection methods are...literature, scientists can use the Data Hub to compare different methods and vectors, and use this data to guide...particle aggregation and methods to prevent its occurrence during vector purification and formulation. Molecular...

... as fixing and permeabilizing.  References and resources References Brown-Harding H (2020) Fixation artifacts...previously hidden within the protein. Created with BioRender.   Permeabilization What is the point?  Antibodies...100, Tween-20, or saponin. Each detergent has different chemical properties that will impact the level...concentration.      Figure 2: Examples of different permeabilization strategies and when to use them...permeabilizing organellar membranes. Created with BioRender.   How do I choose?! Ok, so now you know some...https://doi.org/10.1177/33.8.3894499    Im K, Mareninov S, Diaz MFP, Yong WH (2018) An Introduction to...

...can be used with or as fluorophores in several different ways. Researchers have used oligonucleotides in...were born. Aptabeacons can be created in many different ways, but the general premise is that an aptamer...relying on a lack of fluorescence for readout may render this approach less sensitive in most applications.... As a product of SELEX and FACS, Broccoli is inherently more stable in cells. The new aptamer also has...stable and can go through cycles of denaturation/renaturation. In vivo assays in which protein fluors are...Aptamers can be selected for these small ligands. References 1. Ma, Hanhui, et al. “Multicolor CRISPR labeling...Shiping, et al. “Aptamer-based biosensors.” TrAC Trends in Analytical Chemistry 27(2):108-117 · February...

... to produce abundant, externally fertilized, transparent progeny (Figure 2). These features give it a ...individual to maintain. Also in contrast to mice, the transparent zebrafish embryo develops outside of the mother...researchers to observe changes to these tissues under different experimental conditions. Strategies for generating...sequencing approaches (Harland, 2018). Several different methods have been developed, relying on established...zebrafish—including the available genetic resources and transparency of the embryo—have proven useful in a variety...vertebratedevelopment using optogenetic approaches.     References El-Brolosy, Mohamed A., et al. "Genetic compensation...plasmid collection Read our molecular biology reference Find plasmids for your research ...

...scientists have since adapted it for a host of different proteins and small molecules from a variety of...needs. Herein, we’ll give you a rundown of the different types of ELISA, advantages and disadvantages of...in the sample. This figure was created with BioRender.com  Direct ELISAs A direct ELISA is the quickest...this method, the target antigen competes with a reference antigen for binding to a primary antibody.  While...typically users first coat the multiwell plate with a reference antigen and then they preincubate the antigen-...allow the unbound primary antibody to bind to the reference antigen. The more antigen in the sample, the more... means less antibody available to bind to the reference antigen, producing a weaker signal.  The major...

...tools with a focus on plants.     References and Resources References Karmakar, S., Panda, D., Baig, ...TnpB protein, much like how PAM sites change for different Cas species. For instance, the TnpB from Deinococcus...Deinococcus geothermalis (Dge10TnpB) targets a different TAM (5′-TTAT-3′). The unique TAM requirements ...for plant genome editing using TnpB. The main difference between them lies in the choice of promoter for...tools for predicting efficiency of guides are currently unavailable, you can use TEEP — a tool based on...Engineering the Plant Genome Using CRISPR/Cas9 RNA Interference in Plant Biology: New Tools for an Old Favorite...

...regulated transcription units. Created with BioRender.com.   Planning makes perfect Our first tip,...vectors and parts you will be using during the different levels of your MoClo assembly. Know your process...dictated by four-base overhangs that are paired with different part types (e.g. 5’-GGAG and 3’-TACT flanking ...ensure that these would be interchangeable between different laboratories. Therefore, choosing acceptor vectors... Example Level 1 assembly. The choice between different Level 0 vectors depends on the four-base overhangs...C-terminal tag; TER, terminator. Created with BioRender.com. The Sainsbury Laboratory Golden Gate Cloning...you plate after each transformation and/or use different E. coli strains with higher transformation efficiency...

...colleagues reported that it is possible to reprogram differentiated cells into induced pluripotent stem cells (...benefit when recruiting patients, specifically children and patients with skin disorders for research ...OriP and induces amplification of the episomes concurrently with DNA amplification in the host cell. This...integration-free, karyotypically normal and are able to differentiate into various cell types of all three germ layers...Kushi) Mukherjee. Kusumika (Kushi) Mukherjee is currently a postdoctoral fellow at Massachusetts General...https://www.linkedin.com/in/kmukherjeephd/.     References 1. El Hokayem, Jimmy, Holly N. Cukier, and Derek...Interview with Darrell Kotton Four Factors that Differentiate the Stem Cell Field Sign Up to Write for the...

...per video abstract at 2,947 views. The journals Current Biology (2,500 views), Journal of Number Theory... Addgene Youtube Channel   After watching these trends for a few years, I decided to look at what online...examples for creating effective research videos.  Karen McKee, also known as the science videographer, is...access online journals or even use a computer.  Currently, two of the more popular biology videos are:   ...published in a recent issue of the open access journal Current Biology. Video on We Share Science: http://wesharescience.com...wesharescience.com/pin/7472 Article: http://www.cell.com/current-biology/abstract/S0960-9822(16)30873-9 Youtube:...WeShareScience or email Ryan at [email protected]   References 1. Larivière, Vincent, Yves Gingras, and Éric...

...Emily P. Bentley in December 2024. References and Resources References Anzalone, A. V., Gao, X. D., Podracky...(Anzalone et al., 2019). Plus, it can combine different types of edits with one another. All of this is... An overview of prime editing. Created with BioRender.com. How does it work? First, an engineered ...precise than base editing (in certain circumstances) Current base editors can perform any base-to-base conversion...indels than prime editing. But for positions that aren’t well positioned within the editing window, prime...type, and goal of the experiment can all impose different constraints and affect how you select and design...5.1-fold (though up to 45-fold) higher across different genomic sites compared to PE1 (Anzalone et al....

...research coming out of the lab.  References and resources References Hao, Y., Yan, J., Fraser, C., Jiang...affinity. This extended-open state is key to strengthening ligand binding, mediating cell adhesion and ...then used these antibodies to show the binding preferences of the αV subunit and its β partners. Of interest...antibodies and affinity reagents to study the difference in cell morphology changes induced by integrins...antibodies comes from IPI’s work with the Boston Children’s Hospital and Harvard-based lab of Tim Springer...discovered by yeast display reveal αV subunit pairing preferences with β subunits (p. 2024.01.26.577394). bioRxiv...

...disease postnatally. Now, three papers published concurrently in Science have shown CRISPR can treat a genetic... Even low-frequency editing could make a huge difference in DMD, and it’s estimated that exon skipping...respectively. Although each study used slightly different methods, they each observed the same positive ...necrosis compared to unedited animals. In grip strength, specific force, and other muscle tests, muscle...disease type C. Although the three DMD studies referenced here represent a great step forward for CRISPR...many distinct CRISPR therapies to match these different mutations. For each therapy, effectiveness and...treatments may also be closer than we might expect.   References 1. Kaiser J. CRISPR helps heal mice with muscular...

... you need. Most often, early career scientists aren’t trained to have these types of negotiations. They...all of the major equipment that you use in your current lab first. Generally, major equipment can be defined..., glassware, etc), get a simple list from your current P.I., lab manager, or administrator containing ... Do online searches, and seek advice from your current administrative services, managers, and techs’ financial... is where you can start to identify which items aren’t critical to your research success. If you can get...can still go horribly awry when several factors aren’t taken into consideration. 1. Always focus on the...for the success of your science. If the resources aren’t available, then you’re entering a win-lose situation...

... for in the future! References Bubeck F, Hoffmann MD, Harteveld Z, Aschenbrenner S, Bietz A, Waldhauer...of Cas9 activity.  The field of optogenetics is renowned for enabling precise temporal and spatial control...CRISPR gene activation system, but settled on a different optimal fusion protein combination. The optimized... C-terminal (residues 714-1368, C714) halves, rendering the Cas9 non-functional when split but regaining...absence of light, Cas9 is bound by AcrIIA4 and rendered inactive. In the presence of blue light, AcrIIA4...Alexander Deiters’ lab, on the other hand, took a different approach. These researchers used a genetically...photoactivatable transcription systems to induce neuronal differentiation. Nat Methods 14:963–966 . https://doi.org/10.1038...

...However, many genes have different functions in different cell types, at different times, and in combination...T cell responses to cancer.     References and resources References LaFleur, M. W., Lemmen, A. M., Streeter...combination with different genes. Having CRISPR systems for assessing genes in defined contexts would be quite...

... tissues are nearly transparent. As Verkhusha explained it to me, the transparency window of mammalian...tumor. They wanted to find something better. Transparency window That’s exactly what they reported doing...corals, but all of them fluoresce outside the transparency window of mammalian tissues,” Verkhusha said...near-infrared fluorescent proteins within this transparency window, so now, we could see deeper.” Adding...of the time, the answers will be there.     References: Bright and stable near-infrared fluorescent ...

...Emily P. Bentley in September 2025. References and Resources References Chen, L., Zhu, B., Ru, G., Meng, ...component of the nucleoside inosine. Created with BioRender.com. How does base editing work? Base transition... UGI = uracil DNA glycosylase. Created with BioRender.com. The BE3 system increased editing frequency...APOBEC3G displayed less predictable sequence preferences.  The Liu lab also used natural and engineered...yielded an editor with more flexible sequence preferences, evoAPOBEC1-BE4max, which is significantly more...the development of prime editing, which uses a different mechanism than base editing, has allowed scientists.... A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing. Nature Biotechnology...

... the transcript. Different Cas orthologues and subtypes function somewhat differently and can be more ...Gram-positive bacteria Jason Peters, Oren Rosenberg, and Carol Gross used two different genetic systems, targeting...Transcriptional repression (CRISPRi) in bacteria As RNA interference does not function in bacteria, most efforts ...Clostridium difficile, could be targeted and preferentially depleted from gut. While still far from the...the BE3 that further improves editing rates. References Bikard D, Euler CW, Jiang W, Nussenzweig PM, ...inMycobacterium tuberculosisusing an efficient CRISPR interference system. Nucleic Acids Res 44:e143–e143 . https...

...2025 and updated in August 2025. References and resources References Ahuja, V., Jeyabalan, S., & Tzanakakis... 1: Diagram of AAV components. Created with BioRender.com. AAV vectors have both distinctive capsids...multiple coreceptors, which may be present on different cell types and sometimes vary between target species... 2023). In summary Your choice of AAV vector inherently depends on the design of your experiment. Though...following table summarizes tissue tropism as we currently understand it. Tropism depends on many variables...AAV8, AAV9 In addition to tissue tropism, different AAV serotypes can be useful to evade neutralizing...

...meaningful changes in the lab could make a big difference in our global impact as scientists. Many scientists... boxes Some vendors take back their expanded polystyrene (a.k.a. Styrofoam or cooler boxes). We get these...receiving temperature-controlled reagents. Expanded polystyrene will likely end up in a landfill, but at least...with CO2 emissions. NEB takes back expanded polystyrene boxes for free (in the U.S.). Their cooler boxes... 1976! Millipore Sigma takes back expanded polystyrene boxes for free (in the U.S.). After you receive...Sustainable Practices to the Lab: Easy Action Items References Geyer, Roland, Jenna R. Jambeck, and Kara Lavender...

...the discoveries yet to come.      References and resources References Arbabi-Ghahroudi M (2017) Camelid...Camelids. The declaration is intended to raise awareness of the economic and cultural importance of these...become appealing candidates for the detection of different cancers (Jin, et al. 2023).  So where do all these...mouse-derived single-domain antibodies. Image created in BioRender and adapted from Xu, et al., 2021.   The single-domain...antibody field has come a long way since the serendipitous discovery of heavy-chain only antibodies in ...

...specific compounds, links to publication references, and cross-references with cell line catalogs and collections...be adjusted based on query, reference, and shared allele search preferences to help you identify proximal...entry.) You can look up the cell lines you are currently or planning to use for helpful information on ...to learn more, check out the Cellosaurus FAQ! References Lorsch JR, Collins FS, Lippincott-Schwartz J....

...Swanson a structural biologist and protein engineer currently working at Sanofi Pharmaceuticals in Cambridge... Rare Disorders (NORD). NORD raises money and awareness for rare disease and helps patients find the correct...first met AJ and his family, he did not seem much different than my second son of similar age. When he came...play with my kids like any other youngster. His parents, Kristi and Rico, had to keep him from running ...an organization dedicated to raising funds and awareness of FOP. Please consider running with us or making...is a structural biologist and protein engineer currently working at Sanofi Pharmaceuticals in Cambridge...

...work demonstrates that different repair template strategies activate different HDR sub-pathways like synthesis-dependent...live updates! My most popular tweet from the conference is shown above. The blog post I mentioned, John...post-CRISPR cleavage When I first looked at the conference agenda, I was surprised to see that it would ...Having had time to digest my notes, since the conference, I think I may have finally grasped just how ...Subscribe to CRISPR updates on our blog to keep current with the latest and greatest! Addgene resources...

...sensors offer different levels of apparent dopamine affinity, with 113050 offering high apparent affinity,... various AAV serotypes can be used to achieve different patterns of expression, researchers often need...affinity, and 113049 offering medium apparent affinity. Plasmid Serotype Name 113050 AAV9, AAVrg pAAV-hSyn-GRAB_DA1h...Glutamate sensors iGluSnFR is a useful tool from Loren Looger’s lab at Janelia for neuroscientists to measure...

...National Institutes of Health.     References and Resources References Bradbury A, Plückthun A (2015) Reproducibility...that secrete antibodies in culture and provide renewable reagents with reliable and reproducible production...made available through our usual service (see our current antibody plasmid collection) and we plan to introduce...working with the community to create a fully transparent resource where researchers can easily share, ... Trimmer JS, Görnemann J, Glanville J, Wolf P, Frenzel A, Wong J, Koh XY, Eng H-Y, Lane D, Lefranc M-P...

...redesigning viral packaging plasmids, investigating different viral production strategies or developing novel...silver stain approaches we show that there is no difference in purity or infectivity between the untreated...GFP-expressing AAV particles we did not observe any difference between untreated and sucrose treated cells. ...it will be exciting to test this approach with different capsids. Most importantly, because our AAV yields...spending less time and resources resupplying our current inventory and more time providing new materials...materials to the research community. References 1. Coroadinha, A. S., et al. "Effect of osmotic pressure on...

...specific problem. Every species is so different – an awake monkey is different from a cell in a dish. I always...want to activate two sets of neurons and see how different parts of the brain or the cells within them work...Boyden: I think because neuroscience has so many different goals: to understand how a mouse changes emotions...find ways to make these tools more selective for different ions. Right now, we can modulate electricity. ... click the link in the Addgene Alerts box. References: Independent Optical Excitation of Distinct Neural...

...This post was contributed by guest blogger Søren Hough, a Biochemistry PhD candidate at the University...double-stranded breaks (Tsai et al. 2015). Additionally, current algorithms do not take into account other elements...sequencing (Hendel et al. 2015), a significant difference as CRISPR experiments move closer to the clinic...and Joung 2016. Many thanks to our guest blogger Søren Hough. We additionally thank Monica Sentmanat, Victor...Ajetunmobi for their contributions to this work. Søren Hough is a Biochemistry PhD candidate in the laboratory...https://magazine.scienceforthepeople.org/.     References 1. Fu, Yanfang, et al. "High-frequency off-target..."Quantifying on-and off-target genome editing." Trends in biotechnology 33.2 (2015): 132-140. PubMed PMID...

... Gal4 lines and study gene expression in many different tissue types! Although putting a gene of interest... overexpressed in several cell types by using different Gal4 drivers. It is much easier to mate flies ...one gene in one cell type and another gene in a different cell type (6–8). Although it’s often very easy...the meantime, be sure to browse the publication references and web links from my first post and start designing...jonchowphd but he still reads “#” as a “pound symbol”. References 1. A. H. Brand, N. Perrimon, Targeted gene expression...systems in Drosophila: A synthesis of time and space, Trends Genet. 20, 384–391 (2004). PubMed PMID: 15262411...

...are normally available (as sectors such as forensics currently have too many applicants compared to the ...Michael Koeris' Journey from Grad Student to Entrepreneur Science careers divided by the most pertinent...types of skills show that you fully explore the different options available and then decide on a course ...full time) and what life is like for someone who currently in the position. Questions you’ll want to find...Michael Koeris' Journey from Grad Student to Entrepreneur Learn to Network and Make the Contacts for Your...

...gradients. The current purification protocols for isolating exo-AAVs rely on differential centrifugation...saw that these AAV particles come in a slightly different shape. These AAV particles are often found with...able to infect more cells (as demonstrated in different cellular environments such as retina or glioblastoma...Exo-AAVs have improved transduction profiles in different AAV serotypes both in vitro and in vivo when compared...to inactivation through anti-AAV antibodies. References György, Bence, et al. "Naturally enveloped AAV...

...the liquid. Molecules absorb different wavelengths of light to different degrees and many molecules have...spectrophotometer measures these absorbances using UV-transparent cuvettes. First you measure the absorbance of...the ladder. You then run samples of your DNA at different dilutions and quantify based on band intensities...elute and store your plasmid DNA Learn about the different grades of plasmid DNA preps Resources on Addgene.org...Addgene.org Visit the Molecular Biology Reference Find plasmids for your research Browse protocols for ...