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We narrowed to 610 results for: STO;

Showing: 251 - 300 of 610 results

  1. 2018 #DeckTheLab Contest Winners

    Type
    Blog Post
    Published
    Jan. 15, 2019, 2:19 p.m.
    ... This collection of agar art from Nika Heijmans stood out to the Addgenies. Happy holidays!🎄☃️ More... work always gets done this time of year 😁 we stopped working on our reports/lab stuff for a few minutes...

  2. Celebrating One Million Plasmids Shared!

    Type
    Blog Post
    Published
    Sept. 25, 2018, 2:39 p.m.
    ... edged passed our one million plasmids shared milestone by ending the day with 1,000,002 plasmids shipped...While we are very excited about reaching this milestone, it wouldn’t have been possible without the scientists...

  3. A Scientist's Guide to Ebola

    Type
    Blog Post
    Published
    Oct. 16, 2014, 3:01 p.m.
    ...the virus itself that kills you, but the cytokine storm it ultimately sets off.  The virus works its way...way into immune cells in ways that aren’t fully understood. Much of the action depends on a surface glycoprotein...

  4. 2022 and 2023: Reflections and Looking Forward

    Type
    Blog Post
    Published
    Feb. 23, 2023, 2:15 p.m.
    ... 6,000 biosensors plasmids. Our most exciting milestone of 2022, however, is arguably the launch of our...have many successes to reflect upon, a wealth of history to learn from, and a compelling future to plan ...

  5. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    Published
    March 11, 2014, 5:55 p.m.
    ...fellow blogger Caroline LaManna put it well: "Wow, custom mutations in monkeys already. I know we've discussed... move so fast." On the very same day the monkey story hit the presses, another very different bit of CRISPR...

  6. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    Published
    Jan. 28, 2014, 3:10 p.m.
    ...buffers. The competent cells are then aliquoted and stored at -80oC until we are ready to perform transformations...competent cells are good for at least a year when stored at -80oC.   For each new batch of competent cells...

  7. Science Communication for Everyone

    Type
    Blog Post
    Published
    April 16, 2019, 1:05 p.m.
    ...medical conditions, so these topics have become a cornerstone for The Addictive Brain. Our community has also...Keep your message to the main three points of your story. Interested in learning more? Visit their website...

  8. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    Published
    Dec. 29, 2020, 2:15 p.m.
    ... Best Way to Elute and Store Your Plasmid DNA? by Paolo Colombi Should I store my plasmid in TE, Tris...

  9. Stepping into a Virtual Internship: My Experience at Addgene

    Type
    Blog Post
    Published
    Aug. 13, 2020, 1:21 p.m.
    ... myself to just simply exist without creating a storm. Given all the options out there, I was certain ...Production Specialist and Arthur Guerra who is a Customer Support Specialist. Both of these Addgenies really...

  10. Scientist Networking: What is an Informational Interview?

    Type
    Blog Post
    Published
    July 1, 2014, 3:42 p.m.
    ... you ask for an Informational Interview it is understood to be a nonthreatening request to discuss experience... people to connect with you and to share their stories. Thanks to the Benjamin Franklin Effect, most people...

  11. SciComm with the Experts at Science in the News Part 2

    Type
    Blog Post
    Published
    April 27, 2017, 2:30 p.m.
    ...social context. Episodes are driven by narrative and story telling. Chapter 5: The SITN Blog - Vini and Amy...about some of SITN's other initiatives, email sitnboston@gmail.com. Many thanks to our guests, Vini Mani...

  12. Science as Art

    Type
    Blog Post
    Published
    March 2, 2015, 3:13 p.m.
    ...adapted to inspire to a new generation. Daniel Stoupin is a PhD scientist and photographer based at the...gathering momentum, including in Cambridge and Bristol. If you’re a scientist who spends many hours at...

  13. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    Published
    Feb. 18, 2015, 3:09 p.m.
    ...contributed by Daniel Bauer and Matthew Canver of Boston Children’s Hospital and Harvard Medical School....pediatric hematologist and principal investigator at Boston Children’s Hospital and Harvard Medical School....

  14. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    Published
    July 20, 2017, 2:30 p.m.
    ...small molecules for use in the clinic and thereby restore motor neuron function in spinal muscular atrophy...mesothelioma sufferers. This disease is less widely understood and often misdiagnosed. Like other forms of mesothelioma...

  16. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

    Type
    Blog Post
    Published
    Dec. 5, 2019, 1:49 p.m.
    ...dictate the space. We wanted to comfortably tell our story, to get scientists excited about what Addgene has...redesigning the homepage, and striving to tell our story in better ways, we realized that Blugene needed ...

  17. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    Published
    July 9, 2015, 2:30 p.m.
    ...require a bachelor’s degree if you have a PhD. 4) Customize cover letters and even resumes The cover letter...project managment) your resume should also be customized to highlight different skills and to get the ...

  18. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    Published
    May 12, 2015, 1:32 p.m.
    ...bisporus.) Reporter constructs equivalent to site-STOP-site-myrRFP, with “site” representing the specific...resulted in complete or near-complete excision of the stop sequence. In contrast, Dre recombinase, the close...

  19. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

    Type
    Blog Post
    Published
    Feb. 20, 2014, 4:57 p.m.
    ...lentiviral CRISPR library.  “This enables you to do customized genetic modification on a scale that was really...Using CRISPR/Cas to Edit Disease Out of the Genome History of CRISPR Cas - A tale of survival and evolution...

  20. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    Published
    May 4, 2023, 1 p.m.
    ...and Resources References Fellmann, C., et al., Cornerstones of CRISPR-Cas in drug development and therapy...CRISPR Guide CRISPR Plasmids and Resources CRISPR History and Development for Genome Engineering    ...

  21. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    Published
    April 2, 2020, 6:17 p.m.
    ...the fundamentals. Our mission is to curate a one-stop reference guide for plasmids, so that you can spend...and references, and how to maintain your plasmid stocks. The guide also contains multiple protocols and...

  22. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

    Type
    Blog Post
    Published
    June 11, 2019, 1:16 p.m.
    ...Biorender workspace. Free Biorender accounts can store 5 workspaces at a time and workspaces are only downloadable...scientists 👨🏽‍🔬👩‍🔬! We built this so you could stop struggling to make figures in PPT 🔬 Also, the base...

  23. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    Published
    June 19, 2018, 1:38 p.m.
    ...collapse E. coli re-express arg1 gas vesicles and restore their ultrasound signal. Using acoustic reporter...range of  the ~1011 commensal bacteria per gram of stool for humans. Bourdaeu et al also used arg1 to in ...

  25. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    Published
    July 7, 2016, 2:30 p.m.
    ...sharing services, as well as #happydepositors who stopped by to say hello. Needless to say we all got very...the conference and talked to some scientists who stopped by the booth about amazing work already represented...

  26. Antibodies 101: Epitope Availability

    Type
    Blog Post
    Published
    Jan. 11, 2022, 2 p.m.
    ...interest for which you have several antibodies in stock. Why are there so many options for a single target...samples. If, however, you are performing an immunohistochemistry assay, you may want to rule out monoclonal...

  30. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    Published
    Feb. 14, 2018, 2:36 p.m.
    ...Blugene and I represented Addgene at the recent Keystone meeting on Precision Genome Editing with Programmable...publications CRISPOR gRNA design tool Researchers at the Keystone meeting had great things to say about CRISPOR,...

  31. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ...after subtracting out non-specific binding). Historically, glutathione-S-transferase (GST) fusion proteins...protein folding (tested using real science). Make a stock solution of 2.5 mM biotin (50X) because 5 mM is ...

  34. Plasmids 101: Origin of Replication

    Type
    Blog Post
    Published
    Nov. 11, 2020, 2:23 p.m.
    ...colony and do not subculture directly from glycerol stocks, agar stabs, or liquid cultures. Freshly transformed...plasmid yield that colonies streaked from a glycerol stock. Incubation for 12-16 hours tends to give higher...

  35. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    Published
    Sept. 26, 2017, 12:12 p.m.
    ...network. Which planet will you visit? Where will you stop for refreshment along the way? What ecosystem makes...developmental programs unfold, absorbing the stochasticity of molecular interactions and embedding individual...

  36. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ...this sequence. The energy from this breakage is stored as a covalent bond between the cleaved 3′ DNA strand...are planning to transform right away OR you can store the reaction at -20C overnight. 4. Transform TOPO...

  37. Plasmid Cloning by PCR

    Type
    Blog Post
    Published
    March 29, 2016, 2:30 p.m.
    ... want to clone from the start codon (ATG) to the stop codon (TGA, in this example). Assuming you are amplifying...taking the final 18 bases of the ORF, including the stop codon (5'-TGGCATATCTCGAAGTACTGA-3'), then adding...

  40. Addgene Depositors Get More Citations

    Type
    Blog Post
    Published
    April 20, 2017, 2:30 p.m.
    ... these plasmids. While clearly an outlier, this story had us wondering: is there a larger trend here? ...also get many more citations This isn’t the whole story, though. It would also be great to know how much...

  41. Science Career Options

    Type
    Blog Post
    Published
    Feb. 23, 2017, 1:30 p.m.
    ...are needed in a variety of roles, including: Livestock Breeder Scientific Illustrator Plant Breeder ...hobbies and volunteer experiences, as well as work history can really show that you have some of the abilities...

  42. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    Published
    Dec. 6, 2016, 3:30 p.m.
    ...format. Finally, while we can’t currently produce custom gRNA-containing lentivirus, we are providing lentiviral...packaging plasmids to create your own lentivirus stocks. Our research scientists have provided their standard...

  45. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    Published
    March 14, 2019, 1:54 p.m.
    ...different cellular environments such as retina or glioblastoma). They also found that exo-AAVs were more resistant...brain tumor stromal cells in a mouse model of glioblastoma (Volak et al. 2018). The structure of exosome...

  47. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    Published
    March 1, 2018, 2:11 p.m.
    ... mechanism for this function is not yet fully understood, we can imagine that this transport is probably... possible, use low-bind or siliconized tubes to store samples as it is more difficult for viral particles...

  48. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ...sets. Kellen Andrilenas is a Ph.D. grad student at Boston University studying mammalian immunology, and in... get involved with DIY bio If you’re not in the Boston area but are inspired to get involved, check out...

  49. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ...devices to our own interests with the content we store in them: music, emails, chats, pictures, and apps...acids memorized, but the structures are another story. AMINO has been very helpful when I’ve had to explain...

  50. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    Published
    Sept. 8, 2015, 2:30 p.m.
    ...including synthetic biology. Addgene depositors Christopher Voigt and Jeffrey Tabor have been working on ...given promoter. To harness the power of light, Christopher Voigt’s lab created the first E. coli light-sensitive...
Showing: 251 - 300 of 610 results