We narrowed to 127 results for: 11

1. CRISPR-based Genome Editing Systems For Editing in E. coli

   Type

   Blog Post

   Published

   April 13, 2021, 1:15 p.m.

   ...biosynthesis, we constructed a strain library with 1-11 copies of integrated genes. We obtained a stable ...

2. An Introduction to Adenovirus

   Type

   Blog Post

   Published

   Oct. 3, 2023, 1:15 p.m.

   ...Surface to the Nuclear Pore Complex. PLoS Pathog, 11(6), e1004821. https://doi.org/10.1371/journal.ppat...

3. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

   Type

   Blog Post

   Published

   Oct. 19, 2017, 1:54 p.m.

   ...Phosphotyrosyl Linkage.” Molecular and Cellular Biology 5(11): 3274–79. PubMed PMID: 2427927. PubMed Central PMCID...

4. CRISPR 101: Homology Directed Repair

   Type

   Blog Post

   Published

   Jan. 26, 2023, 2:15 p.m.

   ...CRISPR-Cas9 system. Ran FA, et al. Nature Protocols Nov;8(11):2281-308; (2013). PubMed. A Mouse Geneticist's...

5. Site Directed Mutagenesis by PCR

   Type

   Blog Post

   Published

   Aug. 2, 2016, 2:30 p.m.

   ...Experimental guidelines Primer Design As a rule of thumb, 11 bp of complementary sequence on either side of the...

6. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

   Type

   Blog Post

   Published

   May 5, 2020, 1:15 p.m.

   ... of novel coronavirus SARS-CoV-2 Sensitivity: 4.6-11 copies/microliter of RNA targets in a 40-minute incubation...

7. New Optogenetic Tools for Cytoskeleton and Membrane Control

   Type

   Blog Post

   Published

   Sept. 28, 2023, 1:15 p.m.

   ... GTPase Signaling Toolbox. ACS Synthetic Biology, 11(1), 515–521. https://doi.org/10.1021/acssynbio.1c00604...

8. CRISPR-mediated Plant Base Editors

   Type

   Blog Post

   Published

   Jan. 3, 2019, 1:35 p.m.

   ..." Nature plants (2018): 1. PubMed PMID: 29867128. 11. Li, Chao, et al. "Expanded base editing in rice ...

9. Plasmids 101: Biotinylation

   Type

   Blog Post

   Published

   Nov. 15, 2018, 1:50 p.m.

   ...PubMed PMID:12802011. PubMed Central PMCID: PMC164612. 11. Liou, Y.-R., Wang, Y.-H., Lee, C.-Y., & Li, P.-C...

10. Fluorescent Proteins 101: When GFP lets you down

    Type

    Blog Post

    Published

    Aug. 23, 2018, 12:05 p.m.

    ...PMID: 28533371. PubMed Central PMCID: PMC5468623. 11. Várnai, Péter, and Tamas Balla. "Live cell imaging...

11. Hot Plasmids: Spring 2025

    Type

    Blog Post

    Published

    June 3, 2025, 1:15 p.m.

    ...insertions up to 12 kb — with efficiencies between 5% and 11%. It has higher efficiency and lower indel formation...

12. Hot Plasmids: Summer 2024

    Type

    Blog Post

    Published

    Aug. 27, 2024, 1:15 p.m.

    ...Schematic of workflow. B) Detail of spacer peptides (3HB: 11-nm 3-helix bundle; SAH: 60-nm single alpha helix)...

13. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type

    Blog Post

    Published

    Sept. 1, 2020, 1:15 p.m.

    ... Approaches for Retinal Targeting. Front Neurosci 11: . https://doi.org/10.3389/fnins.2017.00503  Chamberlain...

14. A Guide to Getting Started in Undergrad Research

    Type

    Blog Post

    Published

    June 8, 2021, 1:15 p.m.

    ...Students. Social Psychological and Personality Science 11:647–657 . https://doi.org/10.1177/1948550619882032...

15. Viral Vectors 101: AAV Variables That Matter

    Type

    Blog Post

    Published

    June 1, 2023, 1:15 p.m.

    ...specificity of GFAP transgenes. Neurochemical Research, 29(11 SPEC. ISS.), 2075–2093. https://doi.org/10.1007/s11064...

16. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type

    Blog Post

    Published

    Dec. 15, 2016, 3:57 p.m.

    ...chromosome engineering in Escherichia coli. PNAS 2000 97 (11) 5978-5983; May 16, 2000, doi:10.1073/pnas.100127597...

17. Sequencing Options for CRISPR Genotyping

    Type

    Blog Post

    Published

    Oct. 4, 2016, 2:30 p.m.

    ...biotechnology 29.9 (2011): 816-823. PubMed PMID: 21822255. 11. Wang, Xiaoling, et al. "Unbiased detection of off-target...

18. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type

    Blog Post

    Published

    Oct. 17, 2019, 12:28 p.m.

    ...." Current protocols in pharmacology 75.1 (2016): 11-19. PubMed PMID: 27960028. Van Duyne, Gregory D. ...

19. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type

    Blog Post

    Published

    Aug. 15, 2017, 1:24 p.m.

    ...PMID: 21527931. PubMed Central PMCID: PMC4772972. 11. Salomonnson, Emma, et al. "Cell-based and in vivo...

20. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type

    Blog Post

    Published

    March 21, 2024, 1:15 p.m.

    ...Stains and fluorescent dyes. Nature Cell Biology, 11(S1), S7. https://doi.org/10.1038/ncb1939 Liu, X.,...