We narrowed to 117 results for: ALC

1. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

   Type

   Blog Post

   Published

   Sept. 5, 2017, 1:58 p.m.

   ...two successful mutations in a single cell. We calculated the likelihood of obtaining homo- and heterozygous...

2. Antibodies 101: Normalization and Loading Controls for Western Blots

   Type

   Blog Post

   Published

   April 8, 2025, 1:15 p.m.

   ... later steps in this chain (blue) to infer or calculate things about earlier ones. In practice, multiple...

3. Hot Plasmids: Summer 2024

   Type

   Blog Post

   Published

   Aug. 27, 2024, 1:15 p.m.

   ... S., Davis, J. R., Newby, G. A., Chen, P. Z., Palczewski, K., & Liu, D. R. (2024). Engineered virus-like...

4. CRISPR 101: Validating Your Genome Edit

   Type

   Blog Post

   Published

   Nov. 3, 2022, 12:15 p.m.

   ...clones from that population. You can additionally calculate the minimum number of clones you would have to...

5. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

   Type

   Blog Post

   Published

   Dec. 2, 2019, 2 p.m.

   ...and determine the topology of the mitochondrial calcium uniporter (Martell et al., 2012). It is important...

6. Antibodies 101: Multiplex Immunofluorescence

   Type

   Blog Post

   Published

   May 23, 2023, 1:15 p.m.

   ... the different signals in a certain region or calculate a colocalization factor to assess the spatial ...

7. Plasmids 101: Restriction Cloning

   Type

   Blog Post

   Published

   Feb. 18, 2016, 3:42 p.m.

   ...base pairs for each varies, it is difficult to calculate this based on DNA concentration alone. One method...

8. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

   Type

   Blog Post

   Published

   Oct. 17, 2019, 12:28 p.m.

   ...Hartmann, and Peter Hegemann. "Rhodopsin-regulated calcium currents in Chlamydomonas." Nature 351.6326 (1991...

9. Important Considerations When Using AAVs

   Type

   Blog Post

   Published

   June 13, 2017, 2:30 p.m.

   ...non-enveloped viruses, so they are resistant to alcohol-based disinfectants. A 10% bleach solution should...

10. 9 tips for a successful postdoctoral experience

    Type

    Blog Post

    Published

    Oct. 9, 2018, 12:21 p.m.

    ... that all group leaders must master. While the calculus of these decisions is complex, here are a few ...

11. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type

    Blog Post

    Published

    June 28, 2018, 3:01 p.m.

    ...optimized to achieve high knock-in efficiency. To calculate the tagging efficiency in the transfected cells...

12. Custom CRISPR Screens & the Green Listed Software

    Type

    Blog Post

    Published

    July 11, 2017, 2:30 p.m.

    ...The different labs use different algorithms to calculate which would be the best gRNAs. Interestingly, ...

13. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type

    Blog Post

    Published

    April 25, 2017, 2:30 p.m.

    ..., et al. "Photoactivatable genetically encoded calcium indicators for targeted neuronal imaging." Nature...

14. Antibodies 101: Introduction to Antibodies

    Type

    Blog Post

    Published

    Jan. 19, 2021, 2:15 p.m.

    ...concentration of the known samples is used to calculate the concentration of protein in the experimental...

15. How to Deposit Your Plasmids with Addgene

    Type

    Blog Post

    Published

    Feb. 27, 2024, 2:15 p.m.

    ... Lysine (D123K), lowers biosensor affinity for calcium". Please describe any tags or fusions (e.g. His...

16. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type

    Blog Post

    Published

    Sept. 3, 2020, 12:15 p.m.

    ...light-inducible heterodimerizing cryptochrome 2 (CRY2) and calcium and integrin-binding protein 1 (CIB1) proteins....

17. Deep Dive: qPCR

    Type

    Blog Post

    Published

    Sept. 29, 2022, 1:15 p.m.

    ... Standard Curve?  Amplification Efficacy Calculation Amplification Efficacy Assumptions Standard...