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Showing: 101 - 120 of 256 results

  1. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    Published
    Feb. 18, 2016, 3:42 p.m.
    ...phosphatase you choose. CIP (calf alkaline phosphatase) or SAP (shrimp alkaline phosphatase) are commonly used...

  2. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    Published
    Oct. 8, 2020, 1:15 p.m.
    ...contamination free. The aerosols that are generated (while talking or pipetting) during working in the laminar airflow...mycoplasmas and these can enter the cell culture when you talk, laugh, sneeze or cough. Wear gloves while working...

  3. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...j.mib.2017.05.008 Makarova, K. S., Wolf, Y. I., Alkhnbashi, O. S., Costa, F., Shah, S. A., Saunders, S. ... S., Wolf, Y. I., Iranzo, J., Shmakov, S. A., Alkhnbashi, O. S., Brouns, S. J. J., Charpentier, E., Cheng...

  4. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...Before we start building your first panel, we'll talk about one last principle of color emission. As you...section of this article again. In the beginning, we talked about the relationship between a photon's wavelength...

  5. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...enzymes are horseradish peroxidase protein (HRP) and alkaline phosphates. HRPs are usually preferred because...if you’re conjugating your antibodies yourself. Alkaline phosphates have a linear reaction rate and are...

  6. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...Graybuck, L. T., Daigle, T. L., Sedeño-Cortés, A. E., Walker, M., Kalmbach, B., Lenz, G. H., Morin, E., Nguyen...B., Tobey, I. G., Moncada-Reid, C., Eid, F.-E., Walkey, C. J., Ljungberg, M. C., Lagor, W. R., Heaney,...

  7. A Case Study of Addgene Bioinformatics

    Type
    Blog Post
    Published
    May 25, 2023, 1:15 p.m.
    ...infrastructure, and secure collaboration.  In that post, we talked about Addgene’s data processing needs, our criteria...

  8. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    Published
    April 1, 2022, 1:15 p.m.
    ...experienced the minor yet consistent annoyance of walking into a fly lab and finding ourselves accosted by...

  9. We’re Going Viral This Week!

    Type
    Blog Post
    Published
    Oct. 14, 2019, 1 p.m.
    ...resources available from Addgene. There’s a lot to talk about! And by the way, there will be viral vector...

  11. Addgene at SfN23!

    Type
    Blog Post
    Published
    Nov. 9, 2023, 2:15 p.m.
    ...areas of expertise, we are each equally excited to talk to you, answer any questions, and get feedback on...

  12. The Codon Guide to Synthetic Biology

    Type
    Blog Post
    Published
    Sept. 14, 2023, 1:15 p.m.
    ...full-time in mid-2020. As I left the ivory tower and walked down into its foothills, I began to have conversations...

  13. DIY DNA Ladders from Penn State University

    Type
    Blog Post
    Published
    July 14, 2017, 2:30 p.m.
    ...Techniques Resources on Addgene.org Use the Kit-Free Alkaline Lysis Plasmid Miniprep Protocol Learn How to Analyze...

  17. Summer Fun at Addgene!

    Type
    Blog Post
    Published
    May 12, 2022, 1:15 p.m.
    ...tips for scientific communication (manuscripts, talks, etc…!)  Deep Dives You probably are familiar with...

  20. 2018 #DeckTheLab Contest Winners

    Type
    Blog Post
    Published
    Jan. 15, 2019, 2:19 p.m.
    ...lab went all out to transform their lab into a walking tour of Buddy the Elf’s journey from the North ...
Showing: 101 - 120 of 256 results