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We narrowed to 333 results for: ARIA;

Showing: 101 - 120 of 333 results
  1. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...size_unit":"px","font":"helvetica","fallback":null,"variant":null,"size":18},"button_color":{"color":"#0052a9...to the intended edit rather than some passenger variant that came along with the single cell clone (although...SaCas9, NmeCas9, Cas12a enzymes, and engineered variants thereof offers additional PAM options that can...
  2. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...and specificity. However, there are countless variables that impact an antibody’s affinity and specificity...very similar proteins. Additionally, they require variable expression of your target across samples - it ...it is hard to distinguish between specific, invariable expression and non-specific, background signal, ...
  3. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    Published
    Aug. 5, 2025, 1:15 p.m.
    ...Antibodies can be broken up into smaller parts, the variable regions can be altered for specific functions,...IgG: immunoglobin G; ScFv: single-chain fragment variable; sdAb: single-domain antibody (often referred ...19px;font-family: helvetica;","color":"#FFFFFF","variant":null,"font":"helvetica","style":"font-family: ...
  4. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ... sensitivity Sanger Sequencing Provided 20% (variable) Sequence-level data Low-throughput, inappropriate... Targeted Amplicon Sequencing Provided 0.01% (variable) Sequence-level data, extremely sensitive Do not...sequencing: a comparison of methodologies for KRAS variant detection." Biotechniques 53.3 (2012): 182-188....
  5. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...ensures that all screened plasmids contain a gene variant* and is amenable to both high and low throughput... recently developed a mitochondrially-targeted variant of PSmOrange, a photoswitchable fluorescent protein... Osaka University have developed two new color variants of their Nano-lantern technology, first developed...are designed to complement the original yellow variant, with all 3 having distinct emission spectra suitable...whereby photons emitted by a Renilla luciferase variant are used to excite a fused fluorescent protein,...
  6. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ... with the Gersbach lab plasmids, multiple Cas9 variants are available: wt humanized Cas9, D10A nickase...clone your own entry vectors containing other Cas9 variants to use with the MuLE system. Multiplexing from...Topic Page Get Advice on Choosing The Proper Cas9 Variant for Your Experiment Learn About Multiplexing with...
  7. Deaminet 2026: Breakthroughs in Base Editing, Deaminase Biology, and Therapeutic Translation

    Type
    Blog Post
    Published
    March 10, 2026, 1:14 p.m.
    ...immunotoxicity. PM577a: Prime editing for Wilson's disease Maria Collier (Prime Medicine) presented one of the most...al., 2025), DNA-FISH for chromosomal structural variant analysis, targeted sequencing of computationally... expression (~10% of cells) can reshape entire ovarian tumors via IL 6–mediated paracrine signaling, explaining...
  8. The 12 Days of CRISPR

    Type
    Blog Post
    Published
    Jan. 3, 2023, 2:15 p.m.
    ...CRISPR, Addgene gave to me: Functional Genetic Variants Revealed by Massively Parallel Precise Genome ...CRISPR, Addgene gave to me: High-fidelity Cas13 variants for targeted RNA degradation with minimal collateral...
  9. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ... plasmids and have very little batch-to-batch variation. Confirmation of sequence can be done from relatively...antibodies Higher non-specific binding; lot-to-lot variability Some recombinant antibodies Not always ...antibodies Higher non-specific binding; lot-to-lot variability Recombinant multiclonal antibodies Not ...
  10. Editor's Choice, July 2016

    Type
    Blog Post
    Published
    Aug. 5, 2016, 3 p.m.
    ...editing when compared to the most commonly used Cas9 variant, SpCas9. These properties, combined with other ...in the post, may make Cpf1 the preferred CRISPR variant going forward.   Special Delivery Fluorophore Targeting...
  11. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...antibodies, which have much lower lot-to-lot variability. If you’re running an already-developed assay...Add sonication step to protein lysis Splice variants Check literature Posttranslational modifications...use a less sensitive exposure kit No signal variation in highly expressed proteins Antibody dilutions...
  12. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    Published
    Jan. 11, 2017, 8:47 p.m.
    ...GFP-tagged and un-tagged versions of SpCas9 and further variants replace SpCas9 with SpCas9n (nickase), which nicks...homology directed repair. You can find all of these variants, as well as links to the Zhang Lab's Nature Protocols...
  13. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ...imaging process worked well with the common GFP variants EGFP, mGFP4-T, and S65T GFP, and no red color ...enthusiasts! Addgene has empty backbones containing GFP variants used by Sattarzadeh et al., as well as a wide ...
  14. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    Published
    Dec. 3, 2020, 2:15 p.m.
    ...far been identified: AAV1-11. Nine regions of variability in the cap gene which differ between the AAV ...changes and/or short peptides sequences into these variable loops is one method for changing a capsid’s tropism...
  15. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    Published
    May 18, 2023, 1:15 p.m.
    ...antibodies in the lab, we focus on a lot on the variable domain and not so much on the constant, or Fc,...Casadevall, A. (2016). Ig Constant Region Effects on Variable Region Structure and Function. Frontiers in Microbiology...
  16. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ...Zsuzsanna Izsvak’s lab screened many SB tranposase variants for hyperactivity in mammalian cells. Their efforts...inducible expression vectors were developed into 16 variants with different selection markers (blasticidin,...
  17. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    Published
    Dec. 19, 2019, 2:40 p.m.
    ... plasmids now packaged in AAVrg. jGCaMP7s, the variant with the most sensitive response to action potentials...to measure glutamate levels. iGluSnFR.A184S, a variant with higher affinity to glutamate, has been packaged...
Showing: 101 - 120 of 333 results