... using a dCas9 variant as well. Furthermore, they provide a high-fidelity (HF) variant of the BE3 that...bacteria. The authors were able to engineer a dCas9 variant fused to bacterial enhancer binding protein required...

...CRISPR, Addgene gave to me: Functional Genetic Variants Revealed by Massively Parallel Precise Genome ...CRISPR, Addgene gave to me: High-fidelity Cas13 variants for targeted RNA degradation with minimal collateral...

... plasmids and have very little batch-to-batch variation. Confirmation of sequence can be done from relatively...antibodies Higher non-specific binding; lot-to-lot variability Some recombinant antibodies Not always ...antibodies Higher non-specific binding; lot-to-lot variability Recombinant multiclonal antibodies Not ...

...editing when compared to the most commonly used Cas9 variant, SpCas9. These properties, combined with other ...in the post, may make Cpf1 the preferred CRISPR variant going forward.   Special Delivery Fluorophore Targeting...

...antibodies, which have much lower lot-to-lot variability. If you’re running an already-developed assay...Add sonication step to protein lysis Splice variants Check literature Posttranslational modifications...use a less sensitive exposure kit No signal variation in highly expressed proteins Antibody dilutions...

...GFP-tagged and un-tagged versions of SpCas9 and further variants replace SpCas9 with SpCas9n (nickase), which nicks...homology directed repair. You can find all of these variants, as well as links to the Zhang Lab's Nature Protocols...

...imaging process worked well with the common GFP variants EGFP, mGFP4-T, and S65T GFP, and no red color ...enthusiasts! Addgene has empty backbones containing GFP variants used by Sattarzadeh et al., as well as a wide ...

...capture a diverse range of CTCs to cope with the variation in cancer, not just the CTCs with high marker ... types, like stem cells or cells infected with malaria or other parasites. Addgene: Do you have any particular...

...scale: Look at the mEmerald variants first, followed by the color variants. The mEmerald plasmids offer...

...antibodies in the lab, we focus on a lot on the variable domain and not so much on the constant, or Fc,...Casadevall, A. (2016). Ig Constant Region Effects on Variable Region Structure and Function. Frontiers in Microbiology...

...far been identified: AAV1-11. Nine regions of variability in the cap gene which differ between the AAV ...changes and/or short peptides sequences into these variable loops is one method for changing a capsid’s tropism...

...Zsuzsanna Izsvak’s lab screened many SB tranposase variants for hyperactivity in mammalian cells. Their efforts...inducible expression vectors were developed into 16 variants with different selection markers (blasticidin,...

...fragments (Fabs) (Porter 1959) and single-chain variable fragments (scFvs) (Inbar et al., 1972) are fused...Localization of antibody-combining sites within the variable portions of heavy and light chains. Proceedings...

...Barcodes consist of an 8bp variable region and a 6 bp fixed region. The variable region acts as a unique ...

... antibody that is a Fab fragment, single-chain variable fragment (scFv), or a nanobody is one potential...Affinity Reagents Antibodies 101: Single-Chain Variable Fragment (scFvs) Resources on Addgene.org Recombinant...

... plasmids now packaged in AAVrg. jGCaMP7s, the variant with the most sensitive response to action potentials...to measure glutamate levels. iGluSnFR.A184S, a variant with higher affinity to glutamate, has been packaged...

... their gene sequence may have slight or large variations depending on how closely related these species...percentage were not good predictors. There is not much variation in GC content between many organisms and DNA synthesis...

... different fluorescent protein to various Cas9 variants, SpCas9, NmCas9, and St1Cas9. These fusions localized... and results were consistent with copy number variation (CNV) analysis of the U2OS cells.   Figure...

...Facility Isotype IgG2a Differences:  Distinct variable domains that recognize different epitopes on GFP...hfYFP structure, the group rationally designed GFP variants with similar capabilities. If you’re looking for...

...typically have a wide range of input quantities. This variation is problematic for conventional NGS library prep...range (1ng – 100ng) are normalized to two-fold variation above and below mean read count per sample, and...