Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 567 results for: CHI

Showing: 101 - 120 of 567 results

  1. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    Published
    April 7, 2015, 4:21 p.m.
    ...This post was contributed by Adam Chin-Fatt, a Ph.D. student at the University of Western Ontario. Adam...the overexpression of various metabolites in Escherichia coli or Saccharomyces cerevisiae with few techniques...programs. Thank you to our guest blogger! Adam Chin-Fatt is a Ph.D. student at the University of Western...recombinant pharmaceuticals. Follow him on Twitter @AdamChinFatt     References Qi, L. S. et al. Repurposing...

  2. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    Published
    Aug. 21, 2014, 3:15 p.m.
    ...Presentation Experience”, “Career Achievements”, and “Academic Achievements”. Leaning my CV more towards me...time in the lab, my colleagues and what I was researching, but I decided I needed a new challenge. Looking... business friendly CV that would focus on my achievements, capabilities, courses and office skills. I ...

  3. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    Published
    Dec. 20, 2017, 2:33 p.m.
    ...but not limited to biotechnology, chemistry, architecture, engineering, mathematics, physical sciences...should only focus on your current scientific achievements. Participating in the scientific review process...international recognition for your outstanding achievements in your specific academic field, (2) that you...receipt of major prizes or awards for outstanding achievement Evidence of membership in associations that require...require their members to demonstrate outstanding achievement Evidence of published material in professional...

  4. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    Published
    March 1, 2018, 2:11 p.m.
    ... as many viral particles must infect a cell to achieve visible expression of the protein. However, expression...expression of receptors may not need to be as high to achieve an observable signal in your experiment, therefore...looking for effective gene expression. How do you achieve high titers in the first place? Some AAVs are known...solution. Add a bit less buffer than you would to achieve the exact desired concentration to avoid over-diluting...

  5. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    Published
    Dec. 12, 2019, 2:22 p.m.
    ... need, and we wanted to support all of them — searching, browsing, drilling down, and zooming out — with...that best match a user’s query, and return the matching resources in an order that makes sense to the ...synapsin, hsyn synapsin-1, human synapsin, hSynap. Matching We capture multiple variations of the same information...ID). Depositor suggestions provide shortcuts to matching PI labs and institutions. There are many more ...

  6. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...oligos can be cloned to create a chimeric guide RNA. Find pX330-U6-Chimeric_BB-CBh-hSpCas9. pSpCas9(BB)-2A-...years on their own or through Addgene!) pX330-U6-Chimeric_BB-CBh-hSpCas9 - The S. pyogenes Cas9 (SpCas9) has... This plasmid expresses the S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from the U6...

  7. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    Published
    June 1, 2017, 2:30 p.m.
    ...thanks to our guest bloggers Joachim Goedhard and Marieke Mastop! Joachim Goedhart is an assistant professor...This post was contributed by guest bloggers Joachim Goedhart and Marieke Mastop from the Section of Molecular...fluorescent probes. You can follow him on twitter: @joachimgoedhart.   Marieke Mastop is a PhD candidate at the...

  8. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    Published
    June 15, 2017, 2:30 p.m.
    ...thanks to our guest bloggers Joachim Goedhart and Marieke Mastop! Joachim Goedhart is an assistant professor...This post was contributed by guest bloggers Joachim Goedhart and Marieke Mastop from the Section of Molecular...fluorescent probes. You can follow him on twitter: @joachimgoedhart.   Marieke Mastop is a PhD candidate at the...

  9. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    Published
    Nov. 3, 2020, 2:15 p.m.
    ... Arnold’s lab and Viviana Gradinaru’s lab used machine learning to design new channelrhodopsins that are...channelrhodopsin that’s successful in vivo. Using machine-learning to overcome channelrhodopsin design challenges...challenges, the Arnold and Gradinaru Labs developed a machine-learning model to help them construct “designer...Robinson JE, Mackey ED, Gradinaru V, Arnold FH (2019) Machine learning-guided channelrhodopsin engineering enables...

  10. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    Published
    April 20, 2023, 4 p.m.
    ...wrong, ask yourself, “What next step will help me achieve my purpose? Should I go over this slide again? ...the presentation, at which point you will have achieved your goal. Yay!   You’re only human When it comes... in front of a very confused group of visiting Chinese undergrads. You get nervous and freeze, or forget...your slide deck gets uploaded. Your mic emits screeching feedback. You’re muted on Zoom. Your video conferencing...

  11. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...recognition by MMR machinery and improved editing efficiency. The lab also optimized the architecture and amino...Enhanced prime editing systems by manipulating MMR machinery In their Cell paper, the Liu Lab found that mismatch... developed a system in which the cellular MMR machinery is disrupted via a dominant negative mutation ...

  12. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    Published
    Sept. 28, 2020, noon
    ...CRISPR-Cas9-assisted genome editing in Escherichia coli. Acta Biochimica et Biophysica Sinica. https://doi....a technique that utilizes phage recombination machinery to promote homologous recombination of linear ...resistance E. coli carrying the phage recombineering machinery are first electroporated with pCas9. Then, pCRISPR.../10.1038/nbt.3043 Bikard D, Jiang W, Samai P, Hochschild A, Zhang F, Marraffini LA (2013) Programmable...

  13. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...which uses a much shorter crRNA. Cas12-RNP-PAGE achieved high editing frequency (nearly 100% in some cases...method is fast! These editing frequencies can be achieved with a 30-minute incubation, even with multiplexed...best-performing variant, CAP-Mac, was effective at breaching the blood-brain barrier and delivering a transgene...as well? Our collection includes a mouse and a chimeric rabbit (original mouse Fab region with rabbit ...

  14. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ...Relations Board to allow graduate students who work as teaching or research assistants to join unions. The topic...provide a better deal for students in areas such as teaching salaries, healthcare access, and benefits; however... Genetics Education Project Lisa Coleman, PhD - Chief Diversity Officer Harvard University  The symposium...research: recommendations from the Future of Research Chicago Symposium F1000Research, 2016. Kyle T. Dolan, Joseph...

  15. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type
    Blog Post
    Published
    Oct. 11, 2018, 12:30 p.m.
    ... more than 10 modules often rely on two-step hierarchical approaches using different Type IIS restriction...Our work demonstrates that it is now possible to achieve 20+ fragment assemblies with both robust efficiency...Additional confirmation of accurate assemblies was achieved by the sequencing of plasmids isolated from blue...fragment lacI/lacZ cassette assembly was easily achievable with high levels of transformants and low backgrounds...

  16. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ... efficiency and building prediction tools with machine learning (PRIDICT, PRIDICT2.0).   How to apply...chromatin-based prediction tool (ePRIDICT). In summary, machine learning-based prime editing prediction models ...computational biology. His expertise includes applying machine learning techniques to make sense of biology, with...B. van, Krauthammer, M., & Schwank, G. (2024). Machine learning prediction of prime editing efficiency...

  17. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ... tag, and the endogenous cellular degradation machinery to function. The dTAG is the smallest tag reviewed... and widespread usage, a proteolysis-targeting chimera (PROTAC) was developed for HaloTag. PROTACs are...functions – there are antibodies to it and ways to achieve fluorescence with it. AID The auxin-inducible degron...441. doi: 10.1038/s41589-018-0021-8 Holland AJ, Fachinetti D, Han JS, Cleveland DW. Inducible, reversible...

  18. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...level in vitro. In vivo monitoring has also been achieved with these sensors, but further optimization is...cell level as they are in vitro. Factors like photobleaching, lack of red-shifted indicators, and brightness...electrochromic FRET capable GEVIs, with rhodopsin quenching the added fluor for this two-part fluorescent ...voltage-dependent changes in rhodopsin modulate the degree of quenching of the fluor via FRET. This system is particularly...

  19. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...enabling microscopes to visualize the cellular architecture of whole tissues in 3D with unprecedented detail... through the specimen, which often leads to photobleaching.  Furthermore, point-by-point scanning along...focus light. Therefore, there is much less photo-bleaching or photo-damage than in conventional laser scanning...1007-1009. Pubmed. H.-U. Dodt, U. Leischner, A. Schierloh, N. Jährling, C.P. Mauch,  K. Deininger, et al...

  20. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...antibody production is Komagataella phaffii (a.k.a  Pichia pastoris) because it secretes fewer of its own ...include Human Embryonic Kidney (HEK) cells and Chinese Hamster Ovary (CHO) cells. (L'Abbé et al., 2018...-8730-6_1. PMID: 30242676. Frenzel A, Hust M, Schirrmann T. Expression of recombinant antibodies. Front....org/10.1016/j.cocis.2014.10.002. Randolph TW, Schiltz E, Sederstrom D, Steinmann D, Mozziconacci O, Schöneich...
Showing: 101 - 120 of 567 results