We narrowed to 271 results for: Cad
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TypeBlog PostPublishedJuly 15, 2014, 4:03 p.m....Activation of membrane receptors and signaling cascades conceivably allows the genome to sense the impact...different tissues in normal and disease states, their circadian regulation, as well as in important physiological...
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Meet an Addgenie: Insights into Grad School, Career Paths, and More
TypeBlog PostPublishedJune 25, 2020, 1:15 p.m.... that there is a more emphasis today about non-academia and industry jobs. Since there are a variety of...and collaborative.” She knew she wanted to leave academia, and once she joined an investment club and discovered... -
Educational Planarian Tools for Teaching Developmental Biology Techniques
TypeBlog PostPublishedMay 24, 2018, 1:20 p.m....dsRNA matching a specific mRNA sequence triggers a cascade of events that results in the degradation of the...expression in planarians." Proceedings of the National Academy of Sciences 100.suppl 1 (2003): 11861-11865. PubMed... -
Fluorescent Proteins 101: Introduction to FRET
TypeBlog PostPublishedJune 27, 2017, 1:03 p.m....molecules, detect cellular processes and signaling cascades, quantify mechanical tension (a molecular “spring...fluorescent proteins." Proceedings of the National Academy of Sciences 96.20 (1999): 11241-11246. PubMed PMID... -
Plasmids 101: Degron Tags
TypeBlog PostPublishedMarch 14, 2024, 1:15 p.m....In the last decade, the use of degron tags has become increasingly popular for the modulation of endogenously...degradation of proteins in mammalian cells. Proc Natl Acad Sci U S A. 2012 Dec 4;109(49):E3350-7. doi: 10.1073... -
PhD Applications After COVID
TypeBlog PostPublishedJan. 5, 2023, 2:15 p.m....’s program, the answer might be to continue in academia and pursue a PhD. However, if you’re anything ...process. COVID-19 has changed many aspects of academia, and the PhD application process is certainly ... -
Transferable Skills: Negotiation
TypeBlog PostPublishedMarch 3, 2022, 2 p.m....within and outside of your organization. In my non-academic roles, some examples of negotiation have been:...anywhere in the world. Whether you are serving on academic committees as a tenured professor or signing contracts... -
Experimenting with New Careers while in Grad School
TypeBlog PostPublishedFeb. 25, 2016, 3:30 p.m....PhD programs, mine was geared toward careers in academia or industry. Luckily, I discovered that there ...emphasize the important roles of scientists outside of academia One recent example: Don't call me a dropout: ... -
Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments
TypeBlog PostPublishedSept. 23, 2014, 3:05 p.m....Currently you can find protocols from NEB, various academic labs, and now Addgene. I just began uploading ...does offer free trials and pricing options for academic labs, departments, industry, and more to fit every... -
Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More
TypeBlog PostPublishedJune 12, 2014, 3:30 p.m....started my Twitter account recently inspired by an academic in literature who gave a talk to the women’s research...yourself as a real person, so that students see academics as well-rounded human beings. It’s also a great... -
Viral Vectors 101: The Retroviral Lifecycle
TypeBlog PostPublishedJuly 27, 2023, 1:15 p.m.... the nucleic acid. Proceedings of the National Academy of Sciences of the United States of America, 54...genetic recombination. Proceedings of the National Academy of Sciences, 87(4), 1556–1560. PubMed PMID: 2304918... -
Tips for arabidopsis transformation
TypeBlog PostPublishedOct. 25, 2018, 1:23 p.m....of the National Academy of Sciences of the United States of America. National Academy of Sciences, 112... -
Negative Can Be Positive: Open AAV Data with Addgene
TypeBlog PostPublishedSept. 6, 2022, 1:15 p.m....Pubmed search for “AAV” and “brain” over the past decade gives over 22,000 results - an enormous amount ...L., Muñoz, K. A., Hsu, R., Kalwani, L., Sierra-Mercado, D., Robinson, J. O., Outram, S., Koenig, B. A.... -
QC Sequencing Technologies at Addgene
TypeBlog PostPublishedJuly 15, 2025, 1:15 p.m....sequencing (“Third-generation sequencing”) In the past decade or so, the newest kid on the block in the sequencing...chain-terminating inhibitors. Proceedings of the National Academy of Sciences, 74(12), 5463–5467. https://doi.org... -
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs
TypeBlog PostPublishedDec. 14, 2017, 2:08 p.m....Massachussetts General Hospital. A little over a decade back when Yamanaka and colleagues reported that...reprogramming by transformation-deficient Myc. Proc Natl Acad Sci U S A, 2010. 107(32): p. 14152-7. PubMed PMID... -
Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View
TypeBlog PostPublishedOct. 19, 2017, 1:54 p.m....Ligand-Regulated Site-Specific Recombination.” Proc. Natl. Acad. Sci. USA 92(June): 5940–44. PubMed PMID: 7597057...Ligand-Dependent Chimeric Cre Recombinase.” Proc Natl Acad Sci U S A 92(15): 6991–95. PubMed PMID: 7624356.... -
Bacterial Toxin-antitoxin Systems as Molecular Biology Tools
TypeBlog PostPublishedNov. 1, 2018, 12:35 p.m....plasmid proliferation." Proceedings of the National Academy of Sciences 80.15 (1983): 4784-4788. PubMed PMID...bacterial cell death." Proceedings of the National Academy of Sciences 93.12 (1996): 6059-6063. PubMed PMID... -
The Challenges of Cell Culture
TypeBlog PostPublishedMay 5, 2016, 2:30 p.m....problems surrounding cell culture over the last few decades some pharmacologists have tended to avoid cell ...comparable studies to be performed on a cell line even decades after it is initially isolated. Regrettably this... -
Antibodies 101: Single Chain Fragment Variables (scFvs)
TypeBlog PostPublishedJune 3, 2021, 1:15 p.m....variables (scFvs)? scFvs weren’t developed for another decade when in 1988 they were cloned by two separate labs... Escherichia coli. Proceedings of the National Academy of Sciences 85:5879–5883 . https://doi.org/10.1073... -
Fluorescent Proteins 101: When GFP lets you down
TypeBlog PostPublishedAug. 23, 2018, 12:05 p.m....spectroscopy and microscopy." Proceedings of the National Academy of Sciences 114.23 (2017): 6005-6009. PubMed PMID...luminescence imaging." Proceedings of the National Academy of Sciences (2015): 201418468. PubMed PMID: 25831507...