We narrowed to 181 results for: DAP

1. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

   Type

   Blog Post

   Published

   Nov. 20, 2018, 8:17 p.m.

   ... that exist online, but this approach has been adapted for the identification of physical materials that...

2. Learning Technical Skills at a Lab Central Workshop

   Type

   Blog Post

   Published

   Feb. 20, 2018, 2:30 p.m.

   ...using this kit is: Fragmentation and end repair Adapter Ligation Clean up/Size selection Amplification ...

3. "Build Your Plasmid": The Game - Play to Teach

   Type

   Blog Post

   Published

   July 17, 2018, 12:23 p.m.

   ...for non-commercial reuse. There should be ways to adapt the game to each a variety of subjects at many different...

4. 5 Great Apps for Lab Life

   Type

   Blog Post

   Published

   Sept. 22, 2016, 2:30 p.m.

   ...student, I have relied more and more heavily on adapting my smartphone to help me with my research in terms...

5. Choosing Your Fluorescent Proteins for Multi-Color Imaging

   Type

   Blog Post

   Published

   Oct. 9, 2014, 3 p.m.

   ...and RFP or the Sedat Quad filter set, designed for DAPI / Fluorescein / Rhodamine / Cy5 (e.g. Semrock's)...

6. A Deep Dive into BioBuilder

   Type

   Blog Post

   Published

   Oct. 11, 2017, 1:44 p.m.

   ...with synthetic biology lab kits and lesson plans adapted from her own MIT curriculum. Drawing on current...

7. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

   Type

   Blog Post

   Published

   July 28, 2017, 1:30 p.m.

   ...cell differentiation (a cell type important for adaptive immune responses, which flies do not have!) (4...

8. Luminescent Imaging with Nano-lanterns

   Type

   Blog Post

   Published

   May 25, 2017, 2:30 p.m.

   ...Nano-lantern, showing the technique’s versatility and adaptability to many different contexts. Improving Nano-...

9. Hot Plasmids - February 2022

   Type

   Blog Post

   Published

   Feb. 22, 2022, 2:15 p.m.

   ...mitochondrial (mito-mCherry-1xFLAG) localization. Adapted from Liu et al. 2021. Anti-FLAG and anti-HA...

10. Antibodies 101: Isotypes

    Type

    Blog Post

    Published

    Oct. 19, 2021, 1:15 p.m.

    ... constant domains. This switching helps B cells adapt during the course of an immune response since it...

11. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type

    Blog Post

    Published

    Nov. 5, 2019, 1:28 p.m.

    ...years flew by, but since then, scientists have adapted CRISPR nucleases for many more specific research...

12. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

    Type

    Blog Post

    Published

    June 6, 2019, 12:41 p.m.

    ...group inserted sgRNAs targeting various innate and adaptive immune genes into pXPR_053. They expressed this...

13. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

    Type

    Blog Post

    Published

    May 19, 2020, 1:15 p.m.

    ... in much of the world, we have found ourselves adapting to a new way of doing things. These new processes...

14. CasPEDIA: A Functional Classification of Cas Enzymes

    Type

    Blog Post

    Published

    Jan. 25, 2024, 2:15 p.m.

    ...Comparison of different Cas enzymes using CasPEDIA. Adapted from Adler et al.   You can also search by ...

15. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type

    Blog Post

    Published

    June 2, 2015, 6:06 p.m.

    ...designed for plant expression, PTGs will likely be adaptable to many different biological systems. Although...

16. Plasmids 101: The protein expression toolbox

    Type

    Blog Post

    Published

    Feb. 16, 2023, 7:05 p.m.

    ... Fig. 3 dTag mechanism of protein degradation. Adapted from Nabet et al. Check out Addgene's Lentiviral...

17. Summer SciComm: Preprints

    Type

    Blog Post

    Published

    Aug. 16, 2022, 1:15 p.m.

    ...asapbio.org/preprints-and-covid-19 Ettinger CL, Sadanandappa MK, Görgülü K, Coghlan KL, Hallenbeck KK, Puebla...

18. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type

    Blog Post

    Published

    March 19, 2019, 1:31 p.m.

    ...sequence TOM20 (pTriEX Tom20 mVenus BoPD). In dark adapted cells expressing these plasmids, tgRFP fluorescence...

19. Plasmids 101: Cre-lox

    Type

    Blog Post

    Published

    Jan. 13, 2015, 3:47 p.m.

    ...natural viral life-cycle, and researchers have adapted the components for use in genome manipulation. ...

20. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type

    Blog Post

    Published

    Dec. 14, 2021, 2:15 p.m.

    ...methylation, leaving the target CpG unmethylated. Adapted from Sapozhnikov and Szyf 2021.   Let me leave...