We narrowed to 121 results for: ENDOG

1. To Codon Optimize or Not: That is the Question

   Type

   Blog Post

   Published

   Nov. 12, 2020, 2:15 p.m.

   ...efficiency is correlated with codon bias across all endogenous genes in E. coli and S. cerevisiae (Tuller et...

2. "Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science

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   Blog Post

   Published

   Sept. 9, 2025, 1:15 p.m.

   ...The Promoter Region – Let's Go! Plasmids for Endogenous Gene Tagging in Human Cells Resources on addgene.org...

3. CRISPR 101: Targeting RNA with Cas13a (C2c2)

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   Blog Post

   Published

   Sept. 21, 2017, 2:07 p.m.

   ...new effector (Abudayyeh et al., 2016). In the endogenous CRISPR/Cas9 system, Cas9 uses both a tracrRNA...

4. CRISPR Activation: A Practical Guide

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   Blog Post

   Published

   Aug. 18, 2016, 2:30 p.m.

   ...light-inducible CRISPR-Cas9 system for control of endogenous gene activation. Nat Chem Biol 11:198–200 . https...

5. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

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   Blog Post

   Published

   Oct. 27, 2015, 3 p.m.

   ...gram negative bacteria periplasm. Efforts to tag endogenous genes for proteins in these compartments using...

6. Plasmids 101: Control Plasmids

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   Blog Post

   Published

   April 9, 2015, 3:29 p.m.

   ...produce a similar product, but do not target an endogenous gene in your experimental cells. For example,...

7. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

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   Blog Post

   Published

   April 9, 2019, 1:13 p.m.

   ...CRISPR/Cas mediated HDR) enables the tagging of endogenous genes close to native expression levels. However...

8. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

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   Blog Post

   Published

   March 5, 2019, 1:08 p.m.

   ...XFP can be used to amplify the signal. Imaging endogenous fluorescence in live animals only permits visualization...

9. CRISPR 101: Validating Your Genome Edit

   Type

   Blog Post

   Published

   Nov. 3, 2022, 12:15 p.m.

   ...this method, but your edit doesn’t affect any endogenous restriction enzyme sites? Never fear! You can...

10. Quick Guide to Near-Infrared Fluorescent Proteins

    Type

    Blog Post

    Published

    Oct. 5, 2021, 1:15 p.m.

    ...Table 1) were optimized for efficient binding of endogenous BV.  When imaging NIR FPs, consider using light...

11. Fluorescent Proteins 101: When GFP lets you down

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    Blog Post

    Published

    Aug. 23, 2018, 12:05 p.m.

    ...requiring anaerobic conditions, proteins that bind endogenous fluorescent co-factors can be used. Examples ...

12. Antibodies 101: The Basics of Western Blotting

    Type

    Blog Post

    Published

    March 8, 2021, 2:41 p.m.

    ...demonstrate that the antibody efficiently binds to endogenously expressed protein at physiologically relevant...

13. Viral Vectors 101: AAV Variables That Matter

    Type

    Blog Post

    Published

    June 1, 2023, 1:15 p.m.

    ...Astrocyte-selective AAV gene therapy through the endogenous GFAP promoter results in robust transduction ...

14. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type

    Blog Post

    Published

    July 28, 2016, 2:30 p.m.

    ... promising strategy for linking RSM inputs to endogenous gene expression (2). Our paper (1) explains ...

15. PiggyBac-ing Through the Genome Editing Field

    Type

    Blog Post

    Published

    May 31, 2016, 3:30 p.m.

    ...genome following donor removal, which alters the endogenous DNA sequence and has been suggested to cause ...

16. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type

    Blog Post

    Published

    April 25, 2017, 2:30 p.m.

    ...monomeric state is less likely to interfere with endogenous protein structure and function. By generating...

17. Special Delivery: Fluorophore Targeting for FRET Studies

    Type

    Blog Post

    Published

    July 19, 2016, 2:30 p.m.

    ...2009). However, these reagents can bind numerous endogenous biomolecules in cells nonspecifically, and they...

18. CRISPR 101: Cytosine Transversion Editors

    Type

    Blog Post

    Published

    March 25, 2025, 1:15 p.m.

    .... They conducted a CRISPRi screen to identify endogenous genes that impact CGBE efficiency. In addition...

19. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type

    Blog Post

    Published

    Jan. 13, 2025, 9:49 p.m.

    ...Adamson, B. (2024). Improving prime editing with an endogenous small RNA-binding protein. Nature, 628(8008),...

20. 15 Hot Plasmids from 2017

    Type

    Blog Post

    Published

    Jan. 9, 2018, 3:02 p.m.

    ...into TZAP function, Li and colleagues observed endogenous TZAP localization at telomeres and found that...