We narrowed to 388 results for: HAL;
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  TypeBlog PostPublishedAug. 4, 2020, 1:15 p.m.... J, Jia HP, Halabi C, Sigmund CD, Meyerholz DK, Kirby P, Look DC, Perlman S (2006) Lethal Infection of...
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  25 Work From Home Tips for ScientistsTypeBlog PostPublishedMarch 19, 2020, 1:15 p.m....exploration site Inventing Heron has a radio reporter challenge, and code.org has coding tutorials for all ages...environment. Be kind to yourself. This is a challenging period for everyone for numerous reasons, and...
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  The Power Behind NGS Plasmid Validation: seqWellTypeBlog PostPublishedApril 19, 2017, 3:25 p.m....seqWell, Inc., our mission is to overcome crucial challenges in NGS by developing technologies that can help...problem in multiplex NGS library preparation. The challenge here is that, as more samples are sequenced simultaneously...
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  "Build Your Plasmid": The Game - Play to TeachTypeBlog PostPublishedJuly 17, 2018, 12:23 p.m....Associate Professor at University of Barcelona. The challenge: Making courses on genetic manipulation more hands...could be played when and, depending upon who your challenger was, you might have to drastically alter your...
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  Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging ToolsTypeBlog PostPublishedDec. 10, 2019, 1:58 p.m....these indicators difficult to work with. The Gottschalk lab has surveyed a range of microbial rhodopsin...previously reported for other organisms, and the Gottschalk group hypothesizes that this may be due to different...
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  From Lab Bench to Leadership: Transitioning into Management as a PhDTypeBlog PostPublishedJan. 21, 2025, 2:15 p.m....management, the journey can be as exciting as it is challenging. How do you prepare for this shift and navigate...experiences, and practical advice for overcoming challenges. Experimenting with leadership opportunities...
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  Antibodies 101: ChIPTypeBlog PostPublishedMarch 10, 2022, 3:25 p.m....such as sequencing or qPCR. Yay! Where’s the challenge in that? So while this method is certainly long...the sample type and size.) It can be quite the challenge, and take a few rounds of adjustments, to get ...
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  Top Requested Lentivirus and AAV of 2016TypeBlog PostPublishedJan. 6, 2017, 3:56 p.m....makes generation of high titer Cas9 lentiviruses challenging. This cytotoxicity also causes growth rate variability...):337-47. PubMed PMID: 12595892. 4. Sanjana NE, Shalem O, Zhang F. Improved vectors and genome-wide libraries...
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  A Deep Dive into BioBuilderTypeBlog PostPublishedOct. 11, 2017, 1:44 p.m....engineering designs and ideas ranged from a biological breathalyzer to pesticide breakdown, production of fire-resistant...of students and teachers. It’s possible to join a half-day workshop for teachers, to schedule a three-day...
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  Fluorescent Proteins 101: LuciferasesTypeBlog PostPublishedJuly 22, 2025, 1:15 p.m....Renilla reniformis), click beetle (Pyrophorus plagiophthalamus), deep-sea copepod (Gaussia princeps), Japanese...None No Click Beetle Red (CBR)(Pyrophorus plagiophthalamus) Natural or codon-optimized 613 (luciferin...
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  Plasmids 101: Screens vs. SelectionsTypeBlog PostPublishedNov. 10, 2022, 2:15 p.m....interested in studying genes whose knockout would be lethal to cells and thus not assayable with CRISPR. Another...could culture cells in the drug of interest at a lethal dose for non-manipulated cells and then assay cells...
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  Choosing the B(right)est Fluorescent Protein: PhotostabilityTypeBlog PostPublishedJune 8, 2017, 2:30 p.m....the power 2-fold does not reduce photobleaching by half. How exactly the photobleaching rate depends on ...described by the t½, which is the time after which half of the initial fluorescence intensity remains. The...
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  Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable ProteinsTypeBlog PostPublishedMarch 19, 2019, 1:31 p.m.... (such as Nicotiana benthamiana or Arabidopsis thaliana (Xing et al., 2016)). During Agrobacterium-mediated...structural knowledge of the proteins. To overcome this challenge, the Uppalapati and Woolley lab developed a phage...
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  Pairing CombiGEM and CRISPR for Combinatorial Genetic ScreeningTypeBlog PostPublishedApril 12, 2016, 2:30 p.m....2015; Koike-Yusa et al., 2014; Ma et al., 2015; Shalem et al., 2014; Wang et al., 2014), knock down (Gilbert... 26190106. PubMed Central PMCID: PMC4559080. 8. Shalem, Ophir, et al. "Genome-scale CRISPR-Cas9 knockout...
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  Split Fluorescent Proteins for Studying Protein-Protein InteractionsTypeBlog PostPublishedMay 3, 2022, 1:15 p.m....Tebo et al. Nat Commun. 2019 Arnaud Gautier HaloTag TagBiFC allows exogenous labeling for single-molecule...and reversible complementation. TagBiFC (split HaloTag) provides maximum flexibility in color labeling...
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  How to Prepare for an Industry InterviewTypeBlog PostPublishedJuly 9, 2020, 1:15 p.m....research project. In total, the interviews lasted from half to all of the day. This whole process may seem ...carefully. And even if you don’t have technical challenges, your interviewers might. You’ll probably still...
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  A New Generation of Adenine Base Editors Improves Editing in Primary Human CellsTypeBlog PostPublishedMay 7, 2020, 1:15 p.m....T-to-G•C base changes (Figure 1), but it can be challenging to make these base changes, especially in primary...𝛽-globin), which cause sickle cell disease and 𝛽-thalassemia, can be mitigated with the expression of fetal...
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  Synthetic promoter AAVs for cell-type specific expression in retinal cellsTypeBlog PostPublishedMarch 12, 2020, 1:15 p.m.... at the Institute of Molecular and Clinical Ophthalmology Basel recently took a different approach to ...to test for cell type-specific expression of GFP. Half (113) expressed in retinal cells four weeks post-injection...
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  Early Career Researcher Toolbox: Finding Relevant PapersTypeBlog PostPublishedJan. 9, 2020, 1:45 p.m....particularly hard. There are millions of them. The real challenge is finding relevant papers. The latest installment...topic or that were published by the lab down the hall. Scientific journals: They publish papers, so naturally...
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  Mouse Modeling, Part 2: Breeding and Crossing MiceTypeBlog PostPublishedAug. 6, 2019, 12:55 p.m....that can impact reproduction include embryonic lethality, a short lifespan, or infertility and can arise... number or expression level effects. Another challenge that may arise when crossing mice with multiple...