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  1. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ... BLAST: ExPASy - http://www.expasy.org/genomics/sequence_alignment EMBL-EBI - http://www.ebi.ac.uk/services...services http://www.ebi.ac.uk/Tools/webservices/#multiple_sequence_alignment_msa http://www.ebi.ac.uk...

  2. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...Purification Method Using Glass Syringe Filters. PloS one. https://doi.org/10.1371/journal.pone.0007750  Vogelstein... of the United States of America, 76 2, 615-9 . https://doi.org/10.1073/pnas.76.2.615  Zhou, Y., Zhang...Purification and Gel Extraction Kits. Scientific Reports. https://doi.org/10.1038/s41598-018-30316-w  Zou, Y., Mason...and microbes in under 30 seconds. PLoS biology. https://doi.org/10.1371/journal.pbio.2003916...

  3. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    Published
    Aug. 30, 2022, 1:15 p.m.
    ...artifacts and how to minimize them. FocalPlane. https://focalplane.biologists.com/2020/07/07/fixation-...Journal of Histochemistry & Cytochemistry 33:813–820. https://doi.org/10.1177/33.8.3894499    Im K, Mareninov...Molecular Biology. Springer New York, pp 299–311. https://doi.org/10.1007/978-1-4939-8935-5_26  Rolls G ...Nature of Fixatives in Histology. Leica Biosystems. https://www.leicabiosystems.com/us/knowledge-pathway/fixation-and-fixatives...

  4. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    Published
    Nov. 14, 2017, 2 p.m.
    ...Resources http://www.businessinsider.com/how-to-get-the-most-out-of-job-fairs-2014-3 https://www.forbes.com...surprising-rules-you-should-know-regarding-linkedin-etiquette/5/#4050d70b1e9c https://www.linkedin.com/pulse/20140417174121-34888774...-don-ts Resources for getting into grad school: https://www.gradschools.com/get-informed/grad-school-roadmap...

  5. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ... Ting. Sanchez MI et al., Nat methods. 2019. https://doi.org/10.1038/s41592-019-0665-7 Optimizing ... Addgene! Chertkova, AO, et al., bioRxiv. 2020. https://doi.org/10.1101/160374 Nanobodies- there's a ...purification. Iwaki et al., Protein Expr Purif, 2020. https://doi.org/10.1016/j.pep.2020.105607 A new multiplexed...plasmids! Hazelbaker et al., Sci Reports. 2020. https://doi.org/10.1038/s41598-020-57500-1 The CRISPR...

  6. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...Kingdom of Saudi Arabia. Bioinformation, 14, 53–59. https://doi.org/10.6026/97320630014053 Barlow-Anacker,...increased B cell activation. PLOS ONE, 12, e0180664. https://doi.org/10.1371/journal.pone.0180664 Boix, F.,...recipients. World Journal of Transplantation, 8, 23–37. https://doi.org/10.5500/wjt.v8.i1.23 Jhunjhunwala, S.,...Sterile Implant Materials. PloS One, 10, e0137550. https://doi.org/10.1371/journal.pone.0137550 More resources...

  7. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    Published
    Nov. 3, 2022, 12:15 p.m.
    ... Acids Research Volume 42, Issue 22, Page e168. https://doi.org/10.1093/nar/gku936 Eva K. Brinkman, Arne...Nucleic Acids Research Volume 46, Issue 10, Page e58. https://doi.org/10.1093/nar/gky164 Cancellieri S, Canver...genome editing. (2019) Bioinformatics 36:2001–2008. https://doi.org/10.1093/bioinformatics/btz867 Haeussler... selection tool CRISPOR. (2016) Genome Biol 17: https://doi.org/10.1186/s13059-016-1012-2 Find more CRISPR...

  8. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    Published
    June 8, 2021, 1:15 p.m.
    ...Psychological and Personality Science 11:647–657 . https://doi.org/10.1177/1948550619882032 Chrousos GP, ...syndrome threatens diversity. Science 367:749.2-750 . https://doi.org/10.1126/science.aba8039 Cordova FA (2016...2016) Embrace uncertainty. Science 351:994–994 . https://doi.org/10.1126/science.351.6276.994 Moore E (...Here’s how I built a network of mentors. Science. https://doi.org/10.1126/science.caredit.abi6939 Additional...

  9. X-CHIME: Context Dependent Germline Knockout in Immune Cells

    Type
    Blog Post
    Published
    Jan. 16, 2024, 6:13 p.m.
    ...immune system. Nature Immunology, 25(1), Article 1. https://doi.org/10.1038/s41590-023-01689-6 LaFleur, M....tumor immunity. Nature Immunology 20, 1335–1347. https://doi.org/10.1038/s41590-019-0480-4 LaFleur, M. ...immune system. Nature Communications, 10(1), 1668. https://doi.org/10.1038/s41467-019-09656-2 More resources...

  10. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    Published
    Dec. 12, 2023, 2:15 p.m.
    ... of Norepinephrine. Neuron, 102(4), 745-761.e8. https://doi.org/10.1016/j.neuron.2019.02.037 Kagiampaki...norepinephrine in vivo. Nature Methods, 20(9), Article 9. https://doi.org/10.1038/s41592-023-01959-z Mirdita, M....accessible to all. Nature Methods, 19(6), Article 6. https://doi.org/10.1038/s41592-022-01488-1 More resources...

  11. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    Published
    May 21, 2020, 1:15 p.m.
    ...analysis in Drosophila. Nature Methods 9:47–55 . https://doi.org/10.1038/nmeth.1800 Isaacman-Beck J, Paik...manipulate precise proportions of cells. bioRxiv 788679; https://doi.org/10.1101/788679 Lin R, Wang R, Yuan J, ...of single neurons. Nature Methods 15:1033–1036 . https://doi.org/10.1038/s41592-018-0184-y Additional ...

  12. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    Published
    May 18, 2023, 1:15 p.m.
    ...adaptive immune responses. Cell Res 20, 34–50 (2010). https://doi.org/10.1038/cr.2009.139 Janda, A., Bowen, ...Structure and Function. Frontiers in Microbiology, 7. https://www.frontiersin.org/articles/10.3389/fmicb.2016.00022...specificity. Trends in Immunology, 29(2), 91–97. https://doi.org/10.1016/j.it.2007.11.004 Xu Cao, et al...

  13. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ...Perspective and Future Outlook. Front Immunol 8:1589. https://doi.org/10.3389/fimmu.2017.01589 Jin B, Odongo...Diagnostics and Therapeutics. Int J Mol Sci 24:5994. https://doi.org/10.3390/ijms24065994 Xu J, Xu K, Jung ...neutralize SARS-CoV-2 variants. Nature 595:278–282. https://doi.org/10.1038/s41586-021-03676-z  More resources...

  14. Hot Plasmids - October 2022

    Type
    Blog Post
    Published
    Oct. 6, 2022, 1:15 p.m.
    ...2022). https://doi.org/10.1038/s41593-022-01094-6. (This work was also shared as a preprint: https://doi.org... microscopy. Cell, 18, 3408 - 3425.e29. (2022). https://doi.org/10.1016/j.cell.2022.07.013.   Novel...

  15. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    Published
    Dec. 3, 2020, 2:15 p.m.
    ... Vector for Gene Therapy. BioDrugs 31:317–334 . https://doi.org/10.1007/s40259-017-0234-5  Büning H, Srivastava...Therapy - Methods & Clinical Development 12:248–265 . https://doi.org/10.1016/j.omtm.2019.01.008  Maurer AC,...and Transduction. Human Gene Therapy 31:499–511 . https://doi.org/10.1089/hum.2020.069  Additional resources...

  17. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type
    Blog Post
    Published
    April 23, 2020, 1:15 p.m.
    ...Sequencing. Molecular Therapy - Nucleic Acids 4:e260 . https://doi.org/10.1038/mtna.2015.32 Maynard LH, Smith...Settings. Human Gene Therapy Methods 30:195–205 . https://doi.org/10.1089/hgtb.2019.110 Pacouret S, Bouzelha... Preparations. Molecular Therapy 25:1375–1386 . https://doi.org/10.1016/j.ymthe.2017.04.001 Additional...

  18. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    Published
    March 28, 2022, 4:30 p.m.
    ...multiplex immunolabeling of brain. eLife 8:e43322. https://doi.org/10.7554/elife.43322 Bandrowski, AE, Martone...Transparency of Experimental Methods. Neuron 90(3):434-6. https://doi.org/10.1016/j.neuron.2016.04.030    Bradbury... antibodies used in research. Nature 518:27–29. https://doi.org/10.1038/518027a  Resources We would also...

  19. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

    Type
    Blog Post
    Published
    June 22, 2021, 1:15 p.m.
    ...neutralization by convalescent plasma. Nature 593:142–146. https://doi.org/10.1038/s41586-021-03471-w Garcia-Beltran...disease severity and survival. Cell 184:476-488.e11. https://doi.org/10.1016/j.cell.2020.12.015 Garcia-Beltran...vaccine-induced humoral immunity. Cell 184:2372-2383.e9. https://doi.org/10.1016/j.cell.2021.03.013 Additional ...

  20. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

    Type
    Blog Post
    Published
    May 25, 2021, 2:29 p.m.
    ...antibodies used in research. Nature 518:27–29 . https://doi.org/10.1038/518027a Bradbury AM, Plückthun...validate recombinants once. Nature 520:295–295 . https://doi.org/10.1038/520295b Bradbury ARM, Trinklein... functional variable regions. mAbs 10:539–546 . https://doi.org/10.1080/19420862.2018.1445456 Additional...
Showing: 101 - 120 of 238 results