We narrowed to 269 results for: INO

...Protein? GFP is a ~27 kDa protein consisting of 238 amino acids derived from the crystal jellyfish Aequorea...formed from a maturation reaction of three specific amino acids at the center of the protein (Ser65, Tyr66...

...packaged into adeno-associated virus (AAV).  At 984 amino acids in length, Cas9 from Campylobacter jejuni ...editing than SpCas9 (Kim et al., 2016).  The Acidaminococcus sp. BV3L6 Cas12a, AsCpf1, uses a 5'-TTN PAM...

...Shmakov S, Makarova KS, Semenova E, Minakhin L, Severinov K, Regev A, Lander ES, Koonin EV, Zhang F (2016...Semenova E, Minakhin L, Joung J, Konermann S, Severinov K, Zhang F, Koonin EV (2015) Discovery and Functional...

... a Primer Creating Bacterial Glycerol Stocks Inoculating a Liquid Bacteria Culture Collections ... Primers for PCR Agarose Gel Electrophoresis Inoculating Liquid Bacterial Cultures Restriction Digest ...

... currently for terrestrial frogs. Cnidarian-Dinoflagellate symbionts (Virginia Weis) Sea anemone Aiptasia...Cas in lizards. The Menke Lab was able to make an albino lizard with targeted mutations. Now what? What ...

...der Meer D, Dow D, Buser-Doepner C, Bertotti A, Trusolino L, Stronach EA, Saez-Rodriguez J, Yusa K, Garnett...10.1016/j.cell.2014.09.029 Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib...

...recommended for toxic proteins BL21-A1 Tetracycline Arabinose-inducible expression of T7 RNAP; IPTG may still...expression strain to make sure they are compatible. An arabinose-inducible plasmid will not express in an IPTG ...

...expression Promoter of the arabinose metabolic operon Inducible by arabinose and repressed catabolite repression...

... that bind the Arginine-Glycine-Aspartate (RGD) amino acid sequence in their ligands. This well-known ... antigen-binding fragments (Fabs) that bound an amino acid region displayed by a subset of the RGD-binding...

...GFP called 3,5-difluoro-4-hydroxybenzylidene imidazolinone (DFHBI). DFHBI is membrane permeable and non...fluorescence of 3,5-difluoro-4-hydroxybenzylidene-imidazolinone-2-oxime (DFHO), a fluorophore modified from ...

...palindromic and repetitive sequences, but if present, a minor extension can usually ensure that the 3’-base(s)... of the most popular approaches for introducing minor modifications to a plasmid template. Many thanks...

...peroxide. This radical reacts with electron-rich amino acids such as tyrosine on neighboring proteins resulting...aeolicus, which lacks the DNA-binding domain (233 amino acids) found in the E. coli version. It also contains...

... ara disruption of arabinose metabolism pathway Inability to utilize arabinose as a carbon source ...

...promoter (PLtetO), the TP901 gene downstream of the arabinose (Ara)-inducible promoter (PBAD), and the A118 ...A118 gene downstream of the diacetylphloroglucinol (DAPG)-inducible promoter (PPhlF).  Output Plasmid: ...

...rule of thumb, we avoid target sites that code for amino acids near the N’ terminus of the protein, in order.... Likewise, we avoid target sites that code for amino acids close to the C’ terminus of the protein, to...

...codon sequences that code for the same amino acid) for various amino acids. Essentially, instead of forcing...

...interrogate dsDNA for proper base pairing through minor grove contacts, and thus do not read specific base.... "DNA ligases ensure fidelity by interrogating minor groove contacts." Nucleic acids research 32.15 (...

...intermittent rains, it was weather befitting of the ominous political climate in which American scientists ...health care. And it’s needed most in low-income and minority communities, where these issues hit hardest. According...

...corrections are a better choice (Tichopa, 2003. Marino, 2003) - an important note, as the other most commonly...pitfalls and potential. BioTechniques 26:112–115.Marino JH; Cook P; and Miller KS. 2003. Accurate and statistically...

...Gootenberg, J. S., Konermann, S., Trevino, A. E., Scott, D. A., Inoue, A., Matoba, S., Zhang, Y., & Zhang...