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We narrowed to 272 results for: INO

Showing: 101 - 120 of 272 results
  1. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...(ADAR). Such a system would convert adenine to inosine, which the translational machinery treats like ... ADAR1 and ADAR2, which deaminate adenosine to inosine in RNA, creating a functional A->G change. They...
  2. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...affinity tag, generally a relatively small sequence of amino acids, is basically a molecular leash for your protein...; good for antibody-based detection SUMO ~100 amino acid protein  12 Stability At N-terminus, promotes...
  3. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ...Protein? GFP is a ~27 kDa protein consisting of 238 amino acids derived from the crystal jellyfish Aequorea...formed from a maturation reaction of three specific amino acids at the center of the protein (Ser65, Tyr66...
  4. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...der Meer D, Dow D, Buser-Doepner C, Bertotti A, Trusolino L, Stronach EA, Saez-Rodriguez J, Yusa K, Garnett...10.1016/j.cell.2014.09.029 Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib...
  5. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ...packaged into adeno-associated virus (AAV).  At 984 amino acids in length, Cas9 from Campylobacter jejuni ...editing than SpCas9 (Kim et al., 2016).  The Acidaminococcus sp. BV3L6 Cas12a, AsCpf1, uses a 5'-TTN PAM...
  6. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ... a Primer Creating Bacterial Glycerol Stocks Inoculating a Liquid Bacteria Culture Collections ... Primers for PCR Agarose Gel Electrophoresis Inoculating Liquid Bacterial Cultures Restriction Digest ...
  7. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...Shmakov S, Makarova KS, Semenova E, Minakhin L, Severinov K, Regev A, Lander ES, Koonin EV, Zhang F (2016...Semenova E, Minakhin L, Joung J, Konermann S, Severinov K, Zhang F, Koonin EV (2015) Discovery and Functional...
  8. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...recommended for toxic proteins BL21-A1 Tetracycline Arabinose-inducible expression of T7 RNAP; IPTG may still...expression strain to make sure they are compatible. An arabinose-inducible plasmid will not express in an IPTG ...
  9. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ...palindromic and repetitive sequences, but if present, a minor extension can usually ensure that the 3’-base(s)... of the most popular approaches for introducing minor modifications to a plasmid template. Many thanks...
  10. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...GFP called 3,5-difluoro-4-hydroxybenzylidene imidazolinone (DFHBI). DFHBI is membrane permeable and non...fluorescence of 3,5-difluoro-4-hydroxybenzylidene-imidazolinone-2-oxime (DFHO), a fluorophore modified from ...
  11. Hot Plasmids: February 2026

    Type
    Blog Post
    Published
    Feb. 17, 2026, 2:15 p.m.
    ... for new applications: incorporating artificial amino acids into proteins. The team demonstrated 99% on-target... on-target incorporation of non-standard amino acids into reporter proteins. This feat of bioengineering...
  12. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ... that bind the Arginine-Glycine-Aspartate (RGD) amino acid sequence in their ligands. This well-known ... antigen-binding fragments (Fabs) that bound an amino acid region displayed by a subset of the RGD-binding...
  13. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ...interrogate dsDNA for proper base pairing through minor grove contacts, and thus do not read specific base.... "DNA ligases ensure fidelity by interrogating minor groove contacts." Nucleic acids research 32.15 (...
  14. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...rule of thumb, we avoid target sites that code for amino acids near the N’ terminus of the protein, in order.... Likewise, we avoid target sites that code for amino acids close to the C’ terminus of the protein, to...
Showing: 101 - 120 of 272 results