We narrowed to 134 results for: Lta;
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TypeBlog PostPublishedJune 12, 2014, 3:30 p.m....people to, for example, knock out a gene with simultaneous complementation, with a knockout and expression...
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Which Fluorescent Protein Should I Use?
TypeBlog PostPublishedMay 20, 2014, 2:06 p.m....pHTomato (pH sensors), HyPer (H2O2 sensor), ArcLight (voltage sensor), and iGluSnFr (glutamate sensor). More ... -
Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection
TypeBlog PostPublishedDec. 12, 2013, 3:35 p.m....forward-engineering the central dogma across many genes simultaneously. These parts have been carefully engineered... -
CRISPR 101: RNA Editing with Cas13
TypeBlog PostPublishedJuly 31, 2020, 12:30 p.m.... shrink the construct. They identified ADAR2DD(delta984-1090), which decreases the REPAIRv1 construct ... -
Deep Dive: Fixing and Permeabilizing for Immunofluorescence
TypeBlog PostPublishedAug. 30, 2022, 1:15 p.m....mentioned above, organic solvents can be used to simultaneously fix and permeabilize a sample. But you can ... -
Droplet Digital PCR for AAV Quantitation
TypeBlog PostPublishedJan. 16, 2020, 2:18 p.m....measure the concentration of multiple targets simultaneously. For AAV titration, you should only need to... -
Simplify Cloning with in vivo Assembly
TypeBlog PostPublishedOct. 18, 2018, 12:37 p.m....Any IVA primers can be used in combination to simultaneously perform modifications, simply by adding all... -
Tips for Screening with Yeast Two Hybrid Systems
TypeBlog PostPublishedOct. 22, 2015, 2:30 p.m....Kamuzinzi, R., Ledent, V., Blaudez, D., Sanders, D., Revuelta, J.L., Boles, E., André, D., and Frommer, W.B.... -
Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments
TypeBlog PostPublishedSept. 23, 2014, 3:05 p.m....and dissemination of experimental protocols, simultaneously allowing scientists to actively comment, annotate... -
New Tools Enable CRISPRa for Neuroscience Applications
TypeBlog PostPublishedJuly 2, 2019, 12:51 p.m....PMCID: PMC6412672. Zhou, Haibo, et al. "In vivo simultaneous transcriptional activation of multiple genes... -
Analyzing CRISPR Editing Results with ICE from Synthego
TypeBlog PostPublishedMay 8, 2018, 1 p.m....analysis, which compares hundreds of samples simultaneously. The current version of ICE software can analyze... -
Overcoming the AAV Size Limitation for CRISPR Delivery
TypeBlog PostPublishedSept. 16, 2020, 1 p.m....Constructs containing the ADAR2 truncation ADAR2DD(delta984-1090) are approximately 4.1 kb in length, allowing... -
CRISPR 101: Targeting RNA with Cas13a (C2c2)
TypeBlog PostPublishedSept. 21, 2017, 2:07 p.m....amenable to multiplexing; expressing 5 gRNAs simultaneously produces comparable gene knockdown to single... -
Twenty Years of Sharing: Addgene's Viral Vector Service
TypeBlog PostPublishedJuly 30, 2024, 1:15 p.m....Figure 2). Biosensors, which include calcium and voltage sensors, are the broadest category of viral vector... -
Hot Plasmids: Fall 2024
TypeBlog PostPublishedNov. 21, 2024, 1:30 p.m....target multiple genes in M. abscessus either simultaneously or stepwise, achieving mutation efficiencies... -
CRISPR-based Genome Editing Systems For Editing in E. coli
TypeBlog PostPublishedApril 13, 2021, 1:15 p.m....from the cell. Steps 1-1 and 1-2 can be done simultaneously. Image from Li et al., 2021. The updates... -
Five Popular Model Organisms
TypeBlog PostPublishedApril 11, 2019, 12:39 p.m....often as every three weeks so scientists can simultaneously observe several generations of mice at a time... -
The Fluorescent Vegetables in Aptamer Soup
TypeBlog PostPublishedJan. 29, 2019, 2:27 p.m....of artificial guide RNAs that can be used to simultaneously target cellular RNA to specific intracellular... -
Five Popular Model Organisms, Part 2
TypeBlog PostPublishedSept. 29, 2020, 1:15 p.m....and easily obtainable growing conditions. As a facultative aerobe, it can thrive with or without oxygen.... -
3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells
TypeBlog PostPublishedSept. 5, 2017, 1:58 p.m....pathway. Accomplishing this usually involves simultaneously introducing a homologous DNA repair template...