We narrowed to 127 results for: PIR

1. Tips for Screening with Yeast Two Hybrid Systems

   Type

   Blog Post

   Published

   Oct. 22, 2015, 2:30 p.m.

   ...References 1. Casadaban M.J., Martinez-Arias A., Shapira S.K., Chou J. Beta-galactosidase gene fusions for...

2. Tips from the Repository Trenches: Using Barcodes to Track Samples

   Type

   Blog Post

   Published

   Oct. 6, 2015, 2:30 p.m.

   ...Addgene are DH5α, NEB stable, ccdb survival, and Pir1. The majority of plasmids contain either an ampicillin...

3. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

   Type

   Blog Post

   Published

   May 2, 2019, 12:41 p.m.

   ...so can be used to study aspects of cognition. Spiralians - Crepidula atrasolea (Jonathan Henry) This black...

4. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

   Type

   Blog Post

   Published

   April 2, 2019, 1:44 p.m.

   ...named by a postdoc in Cliff Tabin’s lab who took inspiration from a comic book his daughter was reading. SHH...

5. Rett Syndrome: A History of Research and Therapeutic Outlooks

   Type

   Blog Post

   Published

   Nov. 19, 2020, 2:15 p.m.

   ...exhibit slow head growth, develop seizures, cardio-respiratory dysfunction, distinctive hand movements, and ...

6. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

   Type

   Blog Post

   Published

   July 7, 2020, 1:15 p.m.

   ...populations. To develop this method the lab was inspired by work that used an integrated GFP reporter that...

7. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

   Type

   Blog Post

   Published

   Feb. 18, 2020, 2:15 p.m.

   ...their own labs.  Fried chicken with a side of inspiration  I look back at the meeting with such fondness...

8. Using Video to Share Your Science: We Share Science

   Type

   Blog Post

   Published

   Nov. 3, 2016, 2:30 p.m.

   ...studies on reducing antibiotic prescriptions for respiratory tract infections. The video abstracts provide...

9. The Challenges of Cell Culture

   Type

   Blog Post

   Published

   May 5, 2016, 2:30 p.m.

   ... Crown Publishers, 2010. 2. Gold, Michael. "A conspiracy of cells." State University of New York Press...

10. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type

    Blog Post

    Published

    Oct. 27, 2015, 3 p.m.

    ...for subcellular environment. Currently, she is a SPIRE postdoctoral fellow at the University of North Carolina...

11. Important Considerations in Optogenetics Behavioral Experiments

    Type

    Blog Post

    Published

    Oct. 1, 2015, 2:30 p.m.

    ...science writing. Derek is also a pianist and an aspiring composer. View his blog drsimonsaysscience.org...

12. Plasmids 101: Control Plasmids

    Type

    Blog Post

    Published

    April 9, 2015, 3:29 p.m.

    ...individual or co- transfection, should be determined empirically.  Negative controls: untreated cells, empty vector...

13. Hot Plasmids: Summer 2024

    Type

    Blog Post

    Published

    Aug. 27, 2024, 1:15 p.m.

    ..., E., Serack, F., Harvey, J. W., Brauer, P. P., Pirtle, C. P., Coffey, A., Howard, M., Kamath, N., … &...

14. Using Phosphoserine to Study Protein Phosphorylation

    Type

    Blog Post

    Published

    June 23, 2016, 2:30 p.m.

    ...333.6046 (2011): 1151-1154. PubMed PMID: 21868676. 6. Pirman, Natasha L., et al. "A flexible codon in genomically...

15. How to Negotiate a Successful Lab Start Up Budget

    Type

    Blog Post

    Published

    Sept. 1, 2015, 2:30 p.m.

    ... budget can help you compare the monetary and empirical value that helps you determine whether or not ...

16. Is this the right place for me? 8 tactics for choosing a lab

    Type

    Blog Post

    Published

    Oct. 2, 2018, 12:56 p.m.

    ...Will you learn how to run a lab if that is your aspiration? Will you help or at least observe grant writing...

17. CRISPR 101: Epigenetics and Editing the Epigenome

    Type

    Blog Post

    Published

    June 24, 2020, 5:45 p.m.

    ...methyltransferase from the prokaryote Mollicutes spiroplasma (M. Sss1) (termed MQ1.) The Q147L mutation improves...

18. 9 tips for a successful postdoctoral experience

    Type

    Blog Post

    Published

    Oct. 9, 2018, 12:21 p.m.

    ...new pieces of data, your talk will probably not inspire you or the audience. Ask yourself: What is the...

19. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type

    Blog Post

    Published

    Oct. 27, 2020, 1:15 p.m.

    ...hpRNAs, they must be individually engineered and empirically evaluated for their gene silencing efficacy, ...

20. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type

    Blog Post

    Published

    Oct. 8, 2020, 1:15 p.m.

    ...serum to be used for subculturing. Avoid using expired products or reagents that have been stored for ...