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We narrowed to 279 results for: Plat

Showing: 101 - 120 of 279 results

  1. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    Published
    Jan. 30, 2014, 3:29 p.m.
    ...months. Ampicillin breaks down especially fast and plates should be used witin 1 month for optimal efficiency... them to media hotter than about 55oC and store plates/stocks wrapped in foil if a light-sensitive antibiotic...

  2. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    Published
    Nov. 25, 2013, 10:12 p.m.
    ...creatively to deliver a highly specific, programmable platform well-suited for various innovative biological ...enabling access to multiple plasmids used for various platform applications. For more information on CRISPR/Cas...

  3. Meet the New Addgene Board Members!

    Type
    Blog Post
    Published
    April 28, 2020, 1:14 p.m.
    ...is an Addgene depositor. KSQ uses a CRISPR-Cas9 platform to systematically test the function of all 20,...also interested to see Addgene expand the sharing platform to other reagents in biology. Luk H. Vandenberghe...

  5. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ... Pre-warming your antibiotic-containing plate prior to plating your transformation may allow you to see...

  6. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ...DNA – perfect for transforma­­­tion or as a PCR template. Some of the applications of the NEBuilder HiFi...plasmids can be directly transformed into E.coli for plating, screening and generation of sgRNA. Generation ...

  10. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...receptor), then the transducer could be a microscope, plate reader, or any other instrument which measured GFP...advantages over other mechanisms. For one, many viral platforms, such as AAV or retrovirus, can either permanently...

  12. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ...repair, as there is no requirement for a repair template (sister chromatid or homologue) or extensive DNA...NHEJ-dependent repair (“pop-in” insertion) provided a template containing compatible overhangs is available. ...

  13. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ... not limited to: rapid growth, ease of replica plating and mutant isolation, a well-defined genetic system...complementation is weak. yes   LYS2 L-lysine yes - Grow on plates containing alpha-aminoadipate in the absence of...

  15. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...relevant host biomarkers. The lab validated their platform on clinical stool samples by comparison to qPCR... al. "A low-cost paper-based synthetic biology platform for analyzing gut microbiota and host biomarkers...

  16. The time and cost required to make a plasmid

    Type
    Blog Post
    Published
    March 23, 2023, 1:15 p.m.
    ...reagents and consumables, especially for things like plates and gels, which either don’t scale or have limited...use every single well in a 96-well (or 384-well) plate! How much do repositories help?   Designing precise...

  17. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    Published
    April 6, 2017, 1:02 p.m.
    ...percent of cases, the stem cell will seek out a template for the repair process. By flooding the cell with...using the introduced donor plasmid as a repair template. This results in the insertion of the GFP tag ...

  19. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...requires the much less efficient double stranded templates, rather than the more efficient single-stranded...cellular protein. They used CRISPR with a ssODN template to introduce the small wrmScarlet11 which is less...

  20. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    Published
    Oct. 22, 2019, 12:20 p.m.
    ...The UCLA Library offers some Powerpoint based templates with different column numbers for a good starting...starting point. Adobe also offers free poster templates, although most are vertical.  Large central box...
Showing: 101 - 120 of 279 results