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Showing: 101 - 120 of 367 results

  1. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ...opportunities for investigating genetic mechanisms related to morphogenesis, cell fate specification, and ...activity on NAG PAMS and reduced activity on NGG PAMs relative to wild type. A new genome-wide CRISPR knockout...

  2. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ...target protein are typically required due to the relatively low efficiency of biotinylation, at least in ...less in vivo-biotinylated protein (total amount) relative to proteins that have been biotinylated using ...

  3. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    Published
    Dec. 21, 2017, 2:06 p.m.
    ...control elements Coding sequence features are relatively straightforward to define, but for control elements...fluorescent proteins, which often come in closely related versions that have different properties. To prevent...

  4. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    Published
    March 9, 2017, 3:40 p.m.
    ...scheduling Diverse opportunities to do science related jobs that require no bench work Better resources... important first step. Developing mentoring relationships, especially with peer mentors, to discuss your...

  5. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    Published
    April 25, 2019, 3:01 p.m.
    ...which had a total of 31 amino acid replacements relative to CFP484. Because the excitation and emission...combination results in a 2.6-fold increase in signal relative to mCerulean imaged with a standard CFP set. mTFP1...

  6. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    Published
    March 5, 2020, 2:15 p.m.
    ...all of your data prepared. All of these questions relate to factors that you should have already recorded...reproducible research. Share your data on AAV data hub! Related resources on the Addgene blog Important Considerations...

  7. Summer SciComm: Science Storytelling

    Type
    Blog Post
    Published
    Sept. 16, 2022, 1:15 p.m.
    ...even by introducing a person that the audience can relate to. It doesn’t need to be anything fancy; it just... anthropomorphized animals, or even seemingly unrelated stories that they then tuck the science into. ...

  9. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    Published
    Aug. 31, 2023, 1 p.m.
    ... the digestion of food, while brain tissue is relatively static). To operate both types of implanted ...it - the device has a rechargeable battery, is relatively small, and can be detached easily whenever necessary...

  10. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    Published
    Feb. 20, 2018, 2:30 p.m.
    ...chairs in the hallway for people to take a break and relax. Companies at LabCentral get access to a variety...understand? How does the information I’ll be learning relate to me as an individual and Addgene as a company...

  12. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    Published
    April 19, 2017, 3:25 p.m.
    ...sequencing to validate these constructs, as the relatively low cost and quick turn-around time of Sanger... sequential “pooled library prep”, in which a relatively large number of samples are subjected to a DNA-barcoding...

  13. Celebrate National Book Month with Addgene

    Type
    Blog Post
    Published
    Oct. 3, 2019, 12:47 p.m.
    ...Sacks "Neurologist Oliver Sacks examines the relationship between the human brain and music: how we perceive...roles microbes play in our ecosystem, examining relationships between microbes and insects, mammals, and plants...

  14. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    Published
    April 18, 2019, 12:53 p.m.
    ...chromosome-specific loci in the genome and the guides are relatively unstable.   Figure 1: CRISPRainbow labeling... proteins. (B) CRISPR-Sirius used to visualize relative locations of the subtelomeric region of the pericentromeric...

  16. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...glucose or glycerol). K. phaffii is only distantly related to S. pombe and S. cerevisiae; evolutionarily it.... A unique evolutionary feature of K. phaffii, relative to S. cerevisiae, is that it predominantly uses...

  17. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ...epitopes on GFP Monoclonal antibodies can yield relatively low signal in immunoassays due to only having...tested hfYFP in expansion microscopy as well as correlative light and electron microscopy and observed high...

  18. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    Published
    Dec. 18, 2014, 4:06 p.m.
    ...when the research topic you have done is closely related to the job you are applying for, in which case ...in Experimental Medicine. She is the Community Relations Director for Curiosity Collider (hyperlink https...

  19. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    Published
    June 1, 2017, 2:30 p.m.
    ...several important experimental conditions that are related to the imaging strategy and the sample. Practical...the assay is depicted in figure 1. The strict correlation between the fluorescent protein of interest and...

  20. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ...which can help determine structure and function relationships or, in more practical applications, allow a ... any of a huge variety of ncAAs to study the relationship between structure and function…if you can get...
Showing: 101 - 120 of 367 results