...binding of untagged proteins. Despite being a relatively low frequency amino acid, proteins containing...or function of a single protein? Oftentimes, they rely on protein purification. In this strategy a single...contrast, the polyhistidine tag is very small and rarely affects protein function (Bornhorst 2000).  One...other affinity purification methods where binding relies on a specific protein conformation, the polyhistidine-metal...questions about polyhistidine tags. For more antibody-related information, sign up to receive alerts for antibody...

...DREADDs are ideal for chemogenetics as they are relatively insensitive to their endogenous ligand, acetylcholine... GPCRs for chemogenetic studies to probe the relationship between neurons and specific behaviors. Chemogenetics...

...our engineers (Fig. 2). Creating this guide was relatively easy. I took a picture of virtually every container...each given container, when applicable Any other relevant information Prescribing an official name to all...important documentation, including SOPs or project-related documents, we define new and potentially ambiguous...transferable skills Find all of our science career related blog posts Read our science communication blog ...

...measurement. Like a p24 ELISA, this method is relatively quick, providing results in less than a day, ...

...structure, constructing split variants should be relatively straightforward, but it is also possible to create...

.... insulin). Proteins, unlike DNA which can be relatively easily synthesized, must be produced using complex...protein expression, S. cerevisiae, is the most reliable and frequently used species due to its use as ...

...If the phenotype is corrected, students can be relatively confident that the gene from the test species...

... are enriched relative to controls using Split-BioID-GFP and BioID run on six unrelated proteins. One ...These interactions are often modeled as binary relationships, i.e. protein A interacts with protein B; but...transient PPIs. Many PPI identification techniques rely on protein interactors to stay in close contact ...

...identical antibodies (See Fig 1.). rAbs are a relatively new technology that takes advantage of DNA sequencing...revealing the unreliability of antibodies. There are three main underlying causes of this unreliability: cross-reactivity...sifting through comments and papers to find the most reliable (and cost-effective) antibody for my experiment...the antibody can no longer bind to the target). Unreliable antibodies cause research that cannot be reproduced... of all commercially available antibodies are unreliable (Baker, 2015), causing a huge waste of precious...specific (minimal binding to off-target molecules), reliable (wouldn’t perform differently over time), compatible... thousands of scientists to confidently choose reliable and consistent antibodies for specific markers...

...support scientists wear many hats at this still relatively small nonprofit. If you do go longer than a few...these tips and tricks will apply to any biology-related customer support interaction. Before contacting...

...City The conference was located in the modern, relatively new “Science City” area on the outskirts of Guangzhouon...Rajewsky lab is studying circular RNAs (who knew…I had barely heard of these). These single stranded, covalently...of Georgia studies the CRISPR/Cas system as it relates to its natural role in prokaryotic immunity to ...

...8.2 kb, and in vivo transgene expression ceases relatively quickly due to immune response against AdV. Importantly...

...genomes Compared to humans and mice, flies have a relatively simple genome. They have four chromosomes: the...females do not. Also, males have sex combs on their forelegs which are black, bristly hairs. Females do not...

...attending the session, and what is their background relative to your research? Tailor your language to your... you may receive. People often ask about the relationship of your work to recently published papers or...audience just that. Explain what you do know about a related topic, and let them know while you appreciate the...collaborate with a group who presented on a topic related to your research? Find a way to get in contact ...

... 100-fold excess of wild-type bacteria and the relative frequency of each of the 40 library strains was...

...agarose gel to confirm product presence, size, and relative quantity. End point PCR is most commonly used ...reagents and several heating and cooling steps. PCR relies on heat resistant DNA polymerase from the thermophilic...

...While this method can be time-consuming, it is relatively effective in reducing off-target effects since...

...Ras researchers, one of the most exciting and relatively new resources is the RAS Pathway Clone Collection...cases arise and millions of people die from cancer-related complications each year. Despite these grim statistics...making the results of any such study as clinically relevant as possible. Connecting the research community... initiative to curate our collection of cancer-related plasmids such that each is linked to a known major...

...future edits. In contrast, since Cpf1 cleaves relatively far away from the PAM, NHEJ might retain the ... genetic capacity of ~4.5 kb. At 4.1 kb, SpCas9 barely fits into AAV when you add in a gRNA and regulatory...

...Nicotiana benthamiana. N. benthamiana is a tobacco relative and a good model for plant disease because it ...tools work in any plant? Patron: The MoClo kit is entirely for dicots, not grasses and wheat, but we do work... have your parts. That was the motivation for releasing a large parts kit. Once you have so many parts...