...2 spike variants. “We created a series of spike expressing plasmids which encode spikes found on all of...Addgene.org Find plasmids in Addgene's COVID-19 collection Find SARS-COV-2 pseudotype virus plasmids from Addgene...response. The Balazs lab was quick to deposit their plasmids at Addgene to accelerate ongoing COVID-19 research...research. “I hope that by sharing the plasmids other scientists would be able to expand upon the work and... of several SARS-CoV-2 variants coincide with increased vaccination efforts. So, how effective are the...Neutralization levels against spike pseudovirus decreases depending on the variant. The lowest levels of...different circulating variants. This allowed us to create pseudoviruses that represent lineages and variants...

...Proteins More resources from addgene.org  Plasmids encoding nanobodies at Addgene Sybody Generation Toolbox... 12–15 kDa Ease of cloning and expression from plasmids, due to only needing one open reading frame Ability...highlighted back in 2015, where sdAbs were used to create GFP scaffolds for transcriptional activation.  ... mouse-derived single-domain antibodies. Image created in BioRender and adapted from Xu, et al., 2021....

...You’ve gotten the plasmid encoding your protein of interest from Addgene, transfected it into your target... variety of vendors and you may even find plasmids encoding antibodies from Addgene that you can make ...proteins, use a higher percentage of acrylamide to increase migration resistance and improve separation. For...enhance resolution. In this setup, gels have an increasing range of acrylamide content from top to bottom...

...Addgene.org Plasmid Pooled Libraries Pooled library amplification Zhang Lab CRISPR Plasmids The Human TFome...transcription factor library The human genome encodes approximately 1,800 TFs, with >3,500 unique isoforms...controls. Alternatively, you can order individual plasmids from the library to further investigate specific... of the collection, we’re releasing individual plasmids in batches. If there’s something you’d like that...finding. Transcriptional program changes from this screen successfully differentiated cells resembling all...from Joung et al.)    Putting MORF to work Screens and selections In its current format, the MORF ...library is most immediately useful for TF library screens. Previously engineered TF libraries were not as...

...following components: A plasmid in the host bacterium expressing dCas9 A plasmid in the host bacterium ...within T7 RNAP. A phage encoding the deaminase TadA-7.10 A mutagenesis plasmid controlling the phage mutation...on the expression of gene III to create infectious particles. A plasmid-based genetic circuit in the bacteria...Phage-assisted evolution of base-editing activity. A plasmid-based genetic circuit in the bacteria links the...off-target RNA editing rate of ABE7.10. Find the ABE8e plasmids at Addgene! Why engineer and optimize base editors...editors for your research Read our CRISPR guide Find plasmids for your research ...David Liu’s lab created the first base editor in 2016 (Komor et al., 2016) and since then has been trying...

...Addgene Blog Plasmids 101: Common Lab E. coli Strains Plasmids 101: TOPO Cloning Plasmids 101: Golden ...on the F sex factor plasmid of E. coli, is part of a toxin-antitoxin system encoded by the ccd operon, ...-containing vectors in mind, any plasmid that contains the F plasmid (F’ strains) will also be resistant..., which is responsible for plasmid maintenance during cell division. ccdB codes for the toxic protein ...Cells that lose ccdA through the loss of the F plasmid, succumb to the toxicity of CcdB.   Figure...disrupted allowing cells expressing the recombinant plasmid to propagate. Any cells that contained non-recombinant... of clones that do not contain the recombinant plasmid and therefore makes the cloning process much more...

...of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles.  ... 2024. The authors have deposited AAV plasmids for Cre-dependent and -independent versions that are...selectivity. With 10 plasmids available as a kit, plus 21 optional accessory plasmids, the collection targets...collaborators recently created ASAP5, a faster and more responsive genetically-encoded voltage indicator (...precise plant breeding. Find dicot prime editing plasmids here! Vu, T. V., Nguyen, N. T., Kim, J., Song... proteins for mammalian cell expression. These plasmids will be useful not only for future studies of ...proteins of interest too! Find CLIP, CLAP, and SNAP plasmids here! Smith, E.R., Turner, E.D., Abdelhamid, ...

...Interested in using PTG plasmids in your research? Check out the PTG plasmids or plant expression systems...common to prokaryotes and eukaryotes. Though the plasmids made by Xie et. al are specifically designed for...factor; the pol lll promoter and terminator need be encoded only once, and each <200 bp tRNA-gRNA takes up ....1420294112. Epub 2015 Mar 2. PubMed. Find the plasmids from this publication at Addgene Other Techniques....2014.04.022. Epub 2014 May 15. PubMed.  Find the plasmids from this publication at Addgene Dimeric CRISPR...nbt.2908. Epub 2014 Apr 25. PubMed.. Find the plasmids from this publication at Addgene ...mutations through homologous recombination. To further increase the utility of CRISPR/Cas9, it will be necessary...

...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...highlights from recent CRISPR plasmids. To find all of the CRISPR plasmids available from Addgene, head...head over to our CRISPR Plasmids and Resources page.  Plasmids for delivering CRISPRi components by a single...inducible degron system for C. elegans New CRISPR plasmids New items from the viral service Epigenetic ... Haynes at Emory recently contributed a set of plasmids to the repository that will help epigenetic researchers...researchers custom design their own engineering plasmid tools in about a week. Taking advantage of Golden...

...About Other Hot Plasmid Technologies Read Other Synthetic Biology Posts  Suggest Plasmid Tools for the ...Resources on Addgene.org Read our Hot Plasmids Articles Find Plasmids for Bacterial Expression ...of conditions. They are composed of 4 proteins encoded in a single operon and were first expressed in ...produced R-bodies that could extend at different pHs. Plasmids expressing expressing both wild type and mutant...acssynbio.5b00237. PubMed PMID: 26814170. Find Plasmids from This Publication at Addgene 4. Petroni, ...mutagenesis to extend at different pH values. Using a screen that takes advantage of the fact that coiled R-...both lower and higher pH. We initially used this screen to find R body mutants that couldn’t extend at ...

...of the new plasmids, antibodies, and viral preps in our repository through our Hot Plasmids articles.  ...2 nucleocapsid protein (Plasmid #141391-LV [+N]) or membrane protein (Plasmid #141386-LV [+M], negative...RNA polymerase III transcript, the sgRNAs were encoded on the lentiviral minus strand in the 3’ LTR. These... over the original CROPseq. Find CROPseq-multi plasmids here Walton, R.T., Qin, Y., Blainey, P.C. (2024...potential improvements to the editor. Find PE7 plasmids here!     Figure 2: PE7 improves prime... Ace2N-mNeon-A122D and Voltron2 are available as Cre-dependent AAV1 vectors and are suitable for extended...found that administering these AAVs with a dilute Cre-expressing vector resulted in a sparsely-labeled ...

...of multiple DNA fragments to create TAL effector nucleases. These plasmids were designed to utilize the...-containing plasmid such as pX330. This cloning strategy not only makes it easy to create a single gRNA-expressing...Golden Gate method to create CRISPR/Cas9 constructs, assemble standard plasmids parts in different combinations...Addgene’s plasmids are used with a wide variety of restriction enzyme-based cloning methods. Each method...Entry DNA overhangs may be present in the original plasmid (Option 1) or added using PCR-based amplification...produces constructs with an attB recombination scar encoding eight amino acids, but Golden Gate assembly can...gRNA-expressing plasmid, but it can also be adapted to express multiple gRNAs. Addgene has two Golden Gate-based...

...EsCas13d. Find the Yan et al. plasmids here Find the Konermann et al. Plasmids here How do Cas13d enzymes...case, Konermann et al. targeted MAPT, the gene encoding dementia-associated tau, delivering dCasRx and...S0092-8674(18)30207-1. PubMed PMID: 29551272 Plasmids from this paper are available at Addgene. Yan...S1097-2765(18)30173-4. PubMed PMID: 29551514 Plasmids from this paper are available at Addgene. Learn... Resources on Addgene.org: Find RNA Knockdown Plasmids Check out our CRISPR Guide Read our CRISPR History...RspWYL1 increases both targeted and collateral RNA degradation by RspCas13d. RspWYL1 also increased EsCas13d...Cas13d, both groups used very general bioinformatic screens that looked for a CRISPR repeat array near a putative...

...experiments in planarians with their recent plasmid deposit. These plasmids enable two main types of experiments... cloned into plasmids to investigate for each of these planarian species. These plasmids all have a PR-T4...insert the cDNA into the desired plasmid backbone. The Sánchez lab plasmids allow students and new labs to...appropriate plasmid and dsRNA expression is activated through the  induction of T7 polymerase encoded in the...Resources at Addgene.org Find plasmids from the Sánchez lab Find plasmids for other developmental models...transcription to create dsRNA. Students can produce DNA templates via PCR off of the same plasmid and use these...phenotype that disrupts wildtype locomotion.   Create your own plasmids to investigate other genes in planarians...

...will use plasmids and viral preps from Addgene to further their work. The Association for Creatine Deficiencies...will use Addgene’s RESOLUTE plasmid collection to perform a functional screen in mammalian cells to identify... with the Rare Genomics Institute by providing plasmid-based tools for rare disease research. Congratulations...therapeutic development,” says Iyer. Since the RESOLUTE plasmid collection consists of human and murine solute ...contraction defects. The lab also plans to use CRISPR plasmids to generate deletions of candidate genes in euploid...neurological phenotypes observed.” Using genetically-encoded biosensors and iPSC-derived cortical neurons from...research questions,” Hébert says. In the spirit of plasmid sharing and open science, Hébert plans on building...

...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...write about a recent plasmid deposit please sign up here.     Listen to the Hot Plasmids episode! Expanding.... Various soCoChR plasmids described in this work including the soCoChR-GFP plasmid as well as several...further probe cellular function. The CRY2clust Plasmid can be found here. Park et al. Nat Commun. 2017...several derivatives encoding alternative promoters and/or fluorescent tags are available at Addgene. Shemesh..., the Barrick lab has deposited the pDCAF3-IS5 plasmid containing a synthetic operon that enables the ...

... diverse secretory routes in various conditions. The plasmids and lentiviral vectors encoding these proteins...amazing plasmid technologies developed by our community of depositors. With over 40,000 plasmids avaliable...amazing new plasmid tools that have come through our doors in 2015. Do you have a favorite new plasmid tool ...system plasmids are available through Addgene and are already very popular. Use these plasmids to increase...tRNA/aminoacyl-tRNA synthase pair is encoded on a single plasmid and facilitates the incorporation of ... can visualize: Cell Structure Plasmids: Cell Structure Plasmids: ER mCherry-Sec61 beta BFP-Sec61...luciferase reporter gene plasmid pGL3 pGL3 luciferase reporter gene plasmids have been used extensively...

...apoptosis in vivo, scientists have used genetically encoded fluorescent executioner caspase reporters. Several...these diseases and disorders. You can find the plasmids for FlipGFP and FlipCherry at Addgene. References...Download the fluorescent proteins 101 eBook Read our plasmids 101 blog series Resources on Addgene.org Browse...protein fusions Browse the fluorescent proteins plasmid kits ...self-assembling split GFP (GFP1-10 and GFP11) to increase fluorescence 10-fold after protease activity (... (To et al., 2016). However, the lab wanted to increase the fluorescence range to better study the spatio-temporal...β-strand of GFP, hence the name FlipGFP. FlipGFP increases fluorescence 100-fold compared to other reporters...

...clone the genes from the hybridoma into a plasmid to create a recombinant antibody.     Figure...changes in the nucleic acid sequence of the antibody-encoding genes over time as the hybridoma cells divide....Resource on Addgene.org Check out Addgene’s antibody plasmid collection Read the latest on Addgene's new antibody...of Producing Monoclonal Antibodies, 1999). This creates an immortal cell population that will continue ...hybridomas for monoclonal antibody production. Image created with BioRender.com.   The antibody-producing...Generation of Hybridomas: Permanent Cell Lines Secreting Monoclonal Antibodies. https://www.ncbi.nlm.nih.gov...

...towards successful iEP reprogramming. MettI7a1 encodes an uncharacterized putative methyltransferase. ... on Addgene.org Visit our stem cell page Find plasmids for reprogramming and differentiation Check out...individual cells. Each cell is labeled with 3-4 tags to increase the diversity of the CellTag library. The fixed...detected over a 10-week period. Their expression decreased over time but they were not silenced.    Find ...retroviral overexpression cocktail yielded a three-fold increase of fully reprogrammed cells based on single-cell...