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We narrowed to 109 results for: crispr library

Showing: 101 - 109 of 109 results

  1. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ...temperature-sensitive materials like viral preps and libraries were kept at the appropriate temperature for the...Educational Resource Top Five by Views Guides CRISPR Guide Lentiviral Guide AAV Guide Optogenetics Guide... What is a Plasmid? How to Design Your gRNA for CRISPR Editing The Promoter Region - Let's Go! How to ...primers to help users get started with tools like CRISPR, lentivirus, and optogenetics — and shared the ...Addgene blog Twenty Years of Addgene Sharing: CRISPRTwenty Years of Sharing: Addgene's Viral Vector Service...

  2. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...genome-wide screening with CRISPR and Lentivirus Read about the ClonTracr Library Resources on Addgene.org...shRNA-mediated knockdown, Tet and Cre-regulated expression, CRISPR genome editing, and more. Not surprisingly, we ...

  3. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ...Considerations for Using Pooled Lentiviral CRISPR Libraries. Current Protocols in Molecular Biology, 115... may recall our first 20th anniversary story on CRISPR, where we dove fairly deeply into the different...

  4. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...Addgene Blog CRISPR 101: Homology-Directed Repair CRISPR 101: Cytosine Transversion Editors CRISPR 101: Any...Addgene.org Catch up on your CRISPR background with our CRISPR Guide Browse CRISPR plasmids for base editing...Early CRISPR applications were often limited by the low editing efficiency of homology-directed repair... indels were observed than point modifications. CRISPR base editing persists through multiple cell divisions...editing based on Cas9 off-target activity.  Find CRISPR base editing plasmids at Addgene Improving cytosine...near-PAMless Cas9 variants significantly expands the library of accessible genomic targets for base editors ...which you can learn more about in this blog post: CRISPR 101: RNA Editing with Cas13. Finally, the development...

  6. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...latest work, the team designed and screened a large library of iGluSnFR3 mutants, identifying the two  best-performing...editing, think beyond Cas9 with STITCHR: a combined CRISPR / retrotransposon system with the pithy name "site-specific...site-specific target-primed insertion through targeted CRISPR homing of retroelements." The Abudayyeh-Gootenberg...

  7. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ...sorting (FACS) of E. coli transformed with aptamer libraries, the Jaffrey group selected for a 49-nucleotide... References 1. Ma, Hanhui, et al. “Multicolor CRISPR labeling of chromosomal loci in human cells.” Proc...

  8. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    Published
    March 14, 2017, 2:30 p.m.
    ... first ever CRISPR-based clinical trial in 18 lung cancer patients. This study used CRISPR gene editing...Our Cancer Collection Pages Use the Clon Tracer Library to Research Cancer Drug Resistance Checkout the...

  9. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...delivered: fluorescent proteins, optogenetic sensors, CRISPR elements, etc... *These are only starting recommendations...deposited by the Gradinaru lab. It was selected from a library screened in marmosets. AAV.CAP-Mac effectively ...
Showing: 101 - 109 of 109 results