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We narrowed to 535 results for: epo

Showing: 101 - 120 of 535 results

  1. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    Published
    April 25, 2019, 3:01 p.m.
    ...Allele Biotechnology which allows researchers to deposit plasmids containing the fluorescent protein mNeonGreen...fluorophores from Allele Biotechnology that now can be deposited at Addgene. What makes these fluorescent proteins...enhanced GFP (EGFP)-type termini. mNeonGreen was reported as the brightest monomeric green or yellow fluorescent... or for live cell imaging of fusions and FRET reporters. mTFP1 is also insensitive to physiologically ...to share with the scientific community, please deposit the plasmids at Addgene! References Ai, Hui-wang...

  2. Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

    Type
    Blog Post
    Published
    Nov. 8, 2018, 3:47 p.m.
    ... plasmids in the repository, each verified by sequencing, which makes the repository a convenient source...genes deposited at Addgene were synthetic. But nine years later, over 20% of the genes deposited that ...most common expression system within the Addgene repository is mammalian, but the largest source of unique...

  3. Meet the New Addgene Board Members!

    Type
    Blog Post
    Published
    April 28, 2020, 1:14 p.m.
    ...plasmids and the lab eventually deposited these plasmids at Addgene. While deposits from for profit organizations...Officer at KSQ Therapeutics and is an Addgene depositor. KSQ uses a CRISPR-Cas9 platform to systematically...can be very impactful. At Novartis, Stegmeier deposited the ClonTracer Barcoding Library, a library that...remarkable. I was compelled to be part of that.” He’s deposited AAV constructs for gene delivery. In 2018, he ...

  4. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to ...double-stranded break. The Xiao Wang Lab has recently deposited a set of plasmids that helps identify base edited...plasmids use “Cas9-mediated adenosine transient reporter for editing enrichment”, or XMAS-TREE, to identify...CRISPR plasmids are always being added to the repository. To find all of the CRISPR plasmids available...

  5. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    Published
    March 23, 2017, 2:30 p.m.
    ...Page Thank you to all of our generous, sharing depositors. Over 3,000 laboratories are sharing plasmids...currently sharing viral preps) through the Addgene repository. Without these scientists and the support of ...had to pave the way for this project with many depositors and partners. The Addgene Scientist Team has ...Team acquired permission from relevant plasmid depositors and their institutions to distribute viral preps...preps derived from plasmids in the repository. The Outreach Team has been making sure scientists know ...

  6. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    Published
    Dec. 12, 2019, 2:22 p.m.
    ...have perfected processes to curate the data our depositors share and enrich it with sequencing data; as ...categories — catalog, educational resources, and depositors — each with subcategories such as plasmids and...educational resources, and labs and institutions for depositors (see below). Even if the user experience is similar... match. For example, matches of a name in the depositor field will rank higher than matches of the same...that contains a plasmid with the catalog ID). Depositor suggestions provide shortcuts to matching PI labs...

  7. Pooled Library Amplifications

    Type
    Blog Post
    Published
    May 30, 2024, 1:30 p.m.
    ...If you’re depositing a pooled library with Addgene, you may be surprised to learn that we ask for an ...an amplification protocol with your deposit. This is because repeated amplifications of pooled libraries...amplification protocol for your pooled library deposit. When a requesting scientist receives a pooled... ensure that it works for your library before depositing it with Addgene. If you have any specific questions...amplification protocol, feel free to contact us at deposit@addgene.org. Happy amplifying! More resources ...

  8. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...step. Scorpion chemistry in particular has been reported to work well in the Lightcycler while being relatively...Yes Agarose gel Sunrise primers   Low Reporter and quencher on hairpin structure included in ...much as sixfold variation in expression levels reported (Biassoni, 2014), so you’ll want to ensure that...conceptualize. Just remember to switch them back before reporting the data!   Statistical analysis  Getting...frown upon statistical laxness in all its forms. Reporting your data  Once you’ve done the work to produce...(Bustin, 2009) to ensure you’re recording and reporting the right information so others can reproduce ...

  9. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type
    Blog Post
    Published
    April 2, 2019, 1:44 p.m.
    ... the names. We also have many plasmids in our repository that you can use to study these genes! Groucho... contain the groucho gene? Check out plasmids deposited by the Shivdasani Lab from the Dana Farber Cancer...here! The most popular SHH plasmid however was deposited by none other than Cliff Tabin himself.   Sleeping... see for yourself. We have one plasmid in our repository, plasmid 39624, that contains cmr.   tinman and...on Addgene.org Find plasmids for your research Deposit plasmids at Addgene  ...

  10. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ...readers may locate data supporting the research reported in PLOS publications. The policy was not an overnight...is calculated and placed in the legend. Widely reported difficulties in reproducing experimental data ...datasets are too large to transfer to a journal repository, they are likely too large to be retrieved by...Scientific Data, which aims to act as a peer-reviewed repository for “scientifically valuable” datasets. It likely...likely won’t be long before other data repositories emerge that allow efficient searching and extraction...

  11. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...only 46% could be reproduced. In a recent Nature report from researchers at Amgen, it was stated that only...through all saved data and automated writing of reports. To be able to understand the situation in depth...keep paper records as well as digital, or for reporting on a project – printing your data out can be required... you might need share data with them including reports and protocols. To facilitate this, some ELNs allow...Software for Biology Resources on the Addgene Blog Deposit your plasmids with Addgene and make your work more...

  12. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ...Russell and Hirata from the University of Washington reported a surprising finding - when delivered via AAV ...take up ~6.5 kB if delivered in a single AAV. They report roughly 40% repair efficiency in the K562 leukemia...develop AAVs for your own research and encourage to deposit any new AAV tools you create. Many thanks to our...AAV vector cures haemophilia B mice." Scientific Reports 7.1 (2017): 4159. PubMed PMID: 28646206. PubMed... gene cassettes using AAV donor vectors." Cell reports 20.3 (2017): 750-756. PubMed PMID: 28723575. PubMed...

  13. Twenty Years of Sharing Science!

    Type
    Blog Post
    Published
    Jan. 8, 2024, 3:25 p.m.
    ... When we started in 2004, we were the plasmid repository. In 2016, we added viral vectors, and in 2022...ready-to-use antibodies. Now, we think of ourselves as a repository for DNA-based materials, including our popular... kits and libraries. This DNA-based materials repository contains 139,868 reagents from 5,821 labs in ... system for scientists using materials in our repository, from our customer and science support to our...

  14. Export Control Regulations Lifted for Plasmids Containing Rabies and VSV-G Genes

    Type
    Blog Post
    Published
    Feb. 6, 2025, 2:15 p.m.
    ...Popular Rabies and VSV-G Plasmids Name Purpose Depositing Lab pCMV-VSV-G (Empty Backbone) Envelope ...pHCMV-RabiesG Contains Rabies virus G protein; see depositing paper for more information Miguel Sena-Esteves...pcDNA-SADB19L Contains rabies viral RNA polymerase; see depositing paper for more information Edward Callaway ...tools for AAV tracing, made from rabies plasmids deposited by Ian Wickersham’s lab....

  15. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...at specified loci. Charles Gersbach’s lab has deposited mammalian expression constructs including pcDNA-dCas9...Methyltransferase 3 Alpha (DNMT3A) Vlatka Zoldoš’ lab has deposited pdCas9-DNMT3A-EGFP and pdCas9-DNMT3A-PuroR for...Methyltransferase MQ1 Margaret Goodell's lab has deposited pcDNA3.1-dCas9-MQ1(Q147L)-EGFP, a fusion of dCas9...Demethylase 1 (LSD1) Tatjana Sauka-Spengler's lab has deposited pX330a dCas9-LSD1 for targeted removal of H3K4me1...-dead Staphylococcus aureus Cas9 (Sa-Cas) and deposited plasmid IF311: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-LSD1...

  16. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ... plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles. Here's what... intensity-based Glutamate-Sensing Fluorescent Reporter (iGluSnFR) designed and improved by the Loren ... license. While the GENIE team tested and deposited versions with both PDGFR and NGR fusions for membrane...optogenetic activator channelrhodopsin-2 or fluorescent reporter dTomato under the control of one of seven enhancers...interneurons in mice. A) AAV expression of dTomato reporter (magenta) under the control of the BiPVe3 enhancer...

  17. Celebrating the 2024 Nobel Prize in Chemistry

    Type
    Blog Post
    Published
    Oct. 17, 2024, 1:15 p.m.
    ... fans of all our depositors here at Addgene — your contributions make the repository what it is. So we...we’re thrilled this week to congratulate depositor David Baker, who was awarded the Nobel Prize in Chemistry...pressures during protein evolution. The Baker Lab has deposited multiple Top7 plasmids at Addgene, along with ...

  18. What's New in CRISPR - Winter 2018

    Type
    Blog Post
    Published
    Dec. 18, 2018, 1:17 p.m.
    ... SHERLOCK (specific high-sensitivity enzymatic reporter unlocking) to detect single molecules of RNA or...transcription factors with GFP Kevin White’s lab recently deposited a set of CRISPR plasmids to tag human transcription...If you have a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in...Find CRISPR plasmids by function Find recently deposited plasmids ...

  19. 500,000 Plasmids Shipped and Counting!

    Type
    Blog Post
    Published
    March 26, 2015, 3:24 p.m.
    ...Our plasmid repository succeeds because of our amazing community. The trust of our depositors to allow us...more scientists outside of the US now use the repository – about 50% of plasmids are shipped to labs outside...manage our website, and continue to improve the repository. We love being able to provide a useful (and ...

  20. Make a Splash: Notions of Scientific Impact Are Evolving

    Type
    Blog Post
    Published
    Dec. 5, 2014, 2:54 p.m.
    ... to all articles, Anurag Acharya and colleagues report, the percentage of citations to articles in non-elite...publications. (As an interesting aside, Nature recently reported that the most cited work in history is a 1951 ... the best way to measure impact? As that Nature report also noted, papers detailing Nobel Prize-winning... for the impact of research" (2013) Scientific Reports 3, Article number: 1649 doi:10.1038/srep01649 ...
Showing: 101 - 120 of 535 results