We narrowed to 872 results for: fer

... probes, LDR can reliably detect single-base differences. The original creators of this technique visualized...fidelity mean for a ligase? DNA ligases generally prefer fully Watson-Crick base-paired dsDNA substrates.... The structural/mechanistic reason for this differential is not known for certain, but may have to do...This methodology has been used to rapidly screen buffer conditions for Taq DNA Ligase and their effect ...upstream probes and four down-stream probes, each differing only in the base at the ligation junction. Thus...Sixteen separate pools were prepared, each with a different complement strand covering all 16 possible NN ...each possible pairing resulted in a product of a different length, with products identifiable and quantifiable...

...an antibody but want to know if different combinations or even different chimeras might function better...antibody-based cancer therapeutics.    References and Resources References Chan, A. C., & Carter, P. J. (2010...functions, the constant regions can be altered for different isotypes, and so much more. How you engineer an... variable; sdAb: single-domain antibody (often referred to as nanobodies). Created with BioRender.com....specific goal, like improved specificity. There are different ways to achieve this goal, including error-prone...same general cyclical process (Figure 2). The differences typically come in when generating the library...that contains a variety of degenerate codons (different codon sequences that code for the same amino acid...

...codon usage biases of different organisms. Different species have different preferences for the codons they...the gene may not express well because of these different codon usage biases. But if a gene’s sequence was...could be determined through comparisons with a reference sequence database. Through a series of simulations...Kunjapur says. Percent sequence identity was a different story - the team found that the percent sequence...sequences is Proteobacteria - the most frequent transfer of genes is from Proteobacteria to mammalian expression...natural gene sequence is, the less likely it is transferred into another organism in the lab. Predicting ...and synthetic biology is expanding rapidly.  References 1. Kunjapur, Aditya M., Philipp Pfingstag, and...

... C improved assay reliability. Different primers can have different annealing efficiencies (Wang et ...of samples from different sources should not be compared. If using AAV from different sources in the same...solution, and generally these methods measure different characteristics of the solution. These characteristics...because each sample with be quantified with a different primer pair. Ct values vary based on the amount...and low-concentration samples can also behave differently in PCR, even if both samples fall within the ...absolute quantification by using the universal AAV Reference Standard Material (AAV SFM), which is an AAV sample...addition to the AAV RSM, we include a second AAV reference sample of known titer, which is at a higher titer...

...expect, different companies produce different products and therefore need people with different areas of...life science PhDs can offer: vast practical lab expertise, experience in different scientific fields, and... as: “Can I use this product under conditions different from those specified on its data sheet?” or “Do... questions customers ask, and there are also differences in customers’ personalities and their expectations...do jobs in technical support have? There are different names for technical support roles. Examples are...distinction between these job titles, they may be different regarding the levels of lab experience and scientific...communication skills by working with people from many different backgrounds and realizing that the same approach...

...plasmids are compatible, different CBPs bind to each plasmid type, and different NTPases separate the plasmid...plasmids at Addgene Read our Molecular Biology Reference References  del Solar G, Giraldo R, Ruiz-Echevarría...incompatibility is defined as the inability of different plasmids to be maintained in one bacterial cell...al., 1998).  In cases of compatible plasmids, different negative regulation systems control plasmid replication...regulation system cannot distinguish between the different plasmids. The concentration of negative regulation...diffusion) to make this happen.  There are several different versions of the plasmid partitioning system in...identification issue: the CBPs in the cell can’t tell the difference between plasmids if they have the same centromere-like...

...24, 2025 by Alyssa Shepard.   References and Resources References Beumer, K. J., Trautman, J. K....Cong worked with Addgene Senior Scientist, Matt Ferenc, to organize the CRISPR forum's most popular questions...double nickase system is that you can express two different chimeric gRNAs with the Cas9 nickase which will... like the wildtype Cas9 does. There are a few references for the double nickase system, including one ... my target sequence? A3: The human U6 promoter prefers a 'G' at the transcription start site to have high...system probably have better specificity. A good reference is Ran et al. (2013). As for target selection...most guides would work, but they sometimes have different efficiency). And it is best if the cut site is...

...variants differing in kinetics, affinity, and ∆F/F0. Introduce the two variants in three different membrane-targeting...fluorescent signals upon sensing glutamate in mouse and ferret models. Furthermore, they created three affinity...medium affinity for glutamate), that came in three different colors – blue (SF-Azurite-iGluSnFR), green (SF-iGluSnFR...glutamate signaling events, and accurately differentiating between synaptic vs. extrasynaptic glutamate...Figure 1   Increased versatility through two different variants The yellow (v82) and lime green (v857... Figure 2: Glutamate titration curves for the different iGluSnFR variants. Image adapted from Aggarwal...Figure 3: pH titration curves and ∆F/F0 for the different iGluSnFR variants. Image adapted from Aggarwal...

...can't distinguish the different proteins from each other. To distinguish different target proteins in your...target. By using a mix of different antibody-fluorophore combinations for different markers of interest, you...occur in combination with that reporter, you can infer its presence through detecting the reporter's fluorescence...a process called Fluorescence Resonance Energy Transfer (FRET).   Figure 1: Emission spectra and...commonly used fluorophores. A fluorophore's brightness refers to the signal strength of the emitted photons detected...a global maximum at around 560 nm, leading to different levels of brightness, depending on the laser light's...these aren't strict rules and in practice the differences in brightness levels are often not that great...

... in a different city, you can still develop a successful FRET-based assay since energy transfer only occurs...what's it good for? Fluorescence resonance energy transfer (FRET) is a biophysical interaction between two...first fluorophore (the donor) is excited, it can transfer energy directly via resonance to the second fluorophore...targeting the donor and acceptor fluorophores to the different biomolecules you’d like to test for interaction...that the targeting specificity requirements are different for the donor and acceptor fluorophores. The donor...Å away from the donor doesn’t result in energy transfer and thus, your specific FRET signal is unaffected...unaffected  Due to these fundamental differences in targeting specificity for donor and acceptor fluorophores...

...overexpress or knockdown a gene, you might need different plasmids. Harvard’s DRSC/TRiP Functional Genomics...varying expression levels of your transgene in different fly lines. Also, if there is a chance you need...transgenes in one fly, having the transgenes in different loci allows for genetic recombination so that ...for direct comparisons between lines expressing different variants of YFG because the transgenes are found...remember that you’ll need to integrate them all at different loci. Fortunately, there are many docking sites...docking site is located). This amounts to a small difference of two or three weeks. 3. How would you like ...yourself. Depending on your strategy and the services offered by the company, you might have to map into which...

...digestion be sure that the buffers and temperature are compatible between the different enzymes. Always check...ligation mix in different bacterial strains (stbl2, NEB stable, etc.), and/or use a different temperature ...want to assemble together in a combination of different ways according to your cloning strategy: Restriction...from other DNA fragments and you will remove the buffers used in the previous reactions. This will increase... There are multiple ways you can assemble the different parts of a plasmid based on the cloning strategy...attention in the areas of ligation between the different fragments If you don’t get any positive clone...might want to reconsider your approach, and try a different cloning strategy. Once you've identified potential...

...current job situation. What can a coach do that is different from an adviser, boss or mentor? First, professional... transition out of the academic sphere into a different meaningful career in science. Supervisors in the...speaking at research institutions and at scientific conferences, I see that few scientists are aware of the benefits...few hours of coaching time can make an enormous difference so don’t assume that this will cost a fortune...successful outcome. How do I find a coach? Start with referrals. Are there scientists you know who have had a ...offices or professional organizations can provide referrals. There are bad coaches out there, so finding a...specializes in scientist coaching. Always ask for references and follow up with 3-4 to hear about those clients...

...fear of referencing a wrong section. The antibody screening and development of a focused reference bring...School of Medicine at Mount Sinai.  References and resources References Baker M. Reproducibility crisis: ...), variability (separate batches can perform differently – happens when the antibody is produced from ...a new set of animals), and wrong application (different experiments/conditions can change binding ability...off-target molecules), reliable (wouldn’t perform differently over time), compatible with the desired applications...and accuracy while screening. We also compare different clones of the same target to see if the results...with general non-specific binding. If the sample differs from the negative control, such as showing a very...

...containing structural genes and a transfer plasmid carrying the transfer gene. By dividing the viral elements...it is recommended that you try a few different ratios of transfer, envelope, and packaging plasmids in...associated with producing and using lentivirus and offer some tips and tricks for overcoming these hurdles...transactivator, Tat. For more information on the different generations of lentiviral packaging systems please...Guide. While the third generation packaging systems offer an extra level of safety, they also require a fourth...encoding the structural elements, envelope, and transfer gene are transfected into a packaging cell line...titer. Importantly, optimized conditions for one transfer plasmid are not always the best for others. For...

... Evaluating Gene function by RNA interference (RNAi) RNA interference (RNAi) is a powerful tool for performing... These smaller dsRNA fragments are referred to as small interfering RNAs (siRNA) and they bind to a class...genes. Double-stranded RNA (dsRNA)-mediated RNA interference (RNAi) to knockdown expression of specific gene...morgani, and Phagocata gracillis. There are 11 different genes cloned into plasmids to investigate for ...WISH technique. The expression patterns of 11 different planarian genes (all of which are available through...one is working with, the protocol will slightly differ. For example, Girardia sp. and Dugesia dorotocephalacan...and if you make new plasmids for investigating different planarian genes, we encourage you to deposit them...

...frequently implicated in cancer cell proliferation, survival, differentiation, and apoptosis. For each of these...controlling properties like growth, survival, and differentiation.” https://www.addgene.org/kits/sabatini-wood-cancer-pathway-orfs...cells one at a time, you could identify those that confer resistance to drugs,” in a rapid, straightforward.... “You could use it to screen for pathways that confer any phenotype of interest, not just drug resistance...morphological features, migration, or survival in different contexts.” It’s also possible you could make use...plasmids in living animals, to explore pathways that confer metastatic potential or the ability to survive ... sometimes drug screens and sometimes totally different experiments we never thought of - and that’s thrilling...

...the non-profit sector are transferrable to industry such as technology transfer, communications, medical...skills overtime for success. Many people will have different roles, titles, and responsibilities during their...candidate is ultimately looking for a role in a different functional area, then the research role could ...higher degree. These numerically limited visas are referred to as “cap” subject H-1Bs. The H-1B allows U.S...and therefore the academic H-1B visa cannot be transferred to an industry position. If you cannot get an...a foreign national to have an employer or a job offer in order to obtain permanent residence. The recently...your field. No employer is necessary and no job offer is needed for this category. To qualify, you need...

...cellular proliferation. We have appreciated for some time the causes of this uncontrolled proliferation, in...normally encourage cell survival, growth and proliferation, while tumor suppressors perform the opposite...or, in the case of cancer, uncontrolled cell proliferation. Hence it is imperative to the health of an ...division. The combined effect is unregulated proliferation. Another safety net is our own immune system...cellular pathways involved in the development of different cancers and identifying the roles of key proteins...is the Ras pathway. The term “Ras” collectively refers to 3 proteins (H-Ras, K-Ras, and N-Ras) encoded...approaching the Ras problem by exploiting the different mutant versions of Ras that are most prevalent...

...presenting your research at conferences such as SACNAS and ABRCMS. These conferences have a lot of programming... recruiters will attend these conferences to answer questions and offer application fee waivers. One of...her on Twitter @EleanorWang.     References and resources References Canning EA, LaCosse J, Kroeper KM...neurodegenerative disease because they had close relatives suffering from similar diseases. Others were inspired by...proactive Rather than applying only to posted job offerings, the best research experiences typically come ... is a supportive environment—it makes all the difference in a lab experience. “Mentorship” was a bit of...interested in doing research and attending research conferences. The Barry Goldwater scholarship is also a national...