We narrowed to 462 results for: invs
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TypeBlog PostPublishedNov. 13, 2018, 1:22 p.m....discovery: Unless your academic lab is closely involved with clinical trials or has ties to industry, ...work. The number of steps, decisions and people involved is staggering. The process is complicated. If ...important to you, make sure that your project will not involve proprietary compounds or information. Where publication...
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Genome-wide Screening Using CRISPR
TypeBlog PostPublishedAug. 18, 2015, 2:30 p.m.... lead to a wealth of additional experiments investigating the role of individual genes or entire pathways...a population of mutant cells to identify genes involved in a particular phenotype. CRISPR can be readily...specific classes of human genes. What are the steps involved in a CRISPR screen? Performing a forward genetic... -
Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments
TypeBlog PostPublishedSept. 23, 2014, 3:05 p.m....want the works: organizing reagent storage and inventory, managing orders, tracking students and research...access your protocols at the bench, keep your inventory at your finger tips, and make shopping lists as...protcols, sign-up for shared equipment, and track inventory and orders. Quartzy caters to life science labs... -
Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection
TypeBlog PostPublishedDec. 12, 2013, 3:35 p.m.... don't have good ways of converting typically invisible information into actionable signals. Let's say...sensors. Those sensors could transduce otherwise invisible molecules into regulation of gene expression including...bioeconomy, and that it’s been 40 years since the invention of genetic engineering. What's incredible is the... -
AAVs CREATed for Gene Delivery to the CNS and PNS
TypeBlog PostPublishedSept. 28, 2017, 2:01 p.m....the polyA sequence is inverted only in the Cre-expressing tissues. This inversion creates a template that...Yoo BB, et al. Engineered AAVs for efficient noninvasive gene delivery to the central and peripheral nervous... -
Simplify Cloning with in vivo Assembly
TypeBlog PostPublishedOct. 18, 2018, 12:37 p.m....process. The majority of current cloning techniques involve the assembly of a circular plasmid in vitro, before...Laboratory of Molecular Biology. He is currently investigating the molecular biology of synaptic transmission...and plasticity. Javier García-Nafría is an investigator scientist at the MRC Laboratory of Molecular... -
Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!
TypeBlog PostPublishedDec. 3, 2019, 11:38 a.m....protein involved in neurodegeneration in Parkinson’s disease. Knowing the primary investigator where the...available for distribution. If your experiment involves delivering a calcium biosensor using AAV, you ... -
Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research
TypeBlog PostPublishedJuly 29, 2025, 1:15 p.m....blog post was written by Dr. Kutubuddin Molla, investigator at ICAR-Central National Rice Research Institute...Karmakar et al., 2024; Karmakar et al., 2025). We invite researchers to explore and use our TnpB-based genome...account: @Kutub_joy). Dr. Kutubuddin Molla is an investigator at the ICAR-Central National Rice Research Institute... -
CRISPR 101: Any Base Transversion Editors
TypeBlog PostPublishedApril 1, 2025, 1:15 p.m....editor invented. ABE Adenine Base Editor Converts A → G; the second type of base editor invented. CGBE... CBE; the first type of base transition editor invented. Abasic site A DNA site with the nucleobase... -
What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program
TypeBlog PostPublishedMay 2, 2019, 12:41 p.m....scientist. I recently was invited to attend the first Principal Investigator meeting of the NSF EDGE (... students. You can see why I was excited to be invited to attend. My job is to ensure that, in general... -
An Inside Look into Addgene's Dev Team
TypeBlog PostPublishedJan. 23, 2020, 2:45 p.m....Scrum is that it provides transparency to everyone involved about the status of each project. This creates...example, we recently reduced the number of steps involved during checkout. Demo details for this would be...writing code for common tasks, it would be like re-inventing the wheel over and over again for every single... -
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs
TypeBlog PostPublishedDec. 14, 2017, 2:08 p.m....of collecting fibroblasts, which require a more invasive skin biopsy procedure. This becomes a major benefit...grade hiPSCs, reprogramming methods that do not involve modification of the original genome by integrating...cell. The method also reduces biosafety concerns involved in production and usage of Sendai virus. Reprogramming... -
Career Insights: Technical Support Specialist
TypeBlog PostPublishedNov. 9, 2017, 2 p.m.... This will give you a feeling of what might be involved in such a role. In addition, it can be advantageous...extent there is the need (or opportunity) to get involved in product testing in the lab, to travel to customers...outreach, and more. Technical support roles also involve interacting with other departments such as research... -
The Challenges of Cell Culture
TypeBlog PostPublishedMay 5, 2016, 2:30 p.m....cell lines can become cross contaminated and are invariably much older than those available from biorepositories... NDRG2 Expression Attenuates Proliferation and Invasion in Renal Cancer Cells, Marker-independent identification...Thermofisher Guidelines for Maintaining Cultured Cells Invitrogen Cell Culture Basics Guide Abcam Cell Culture ... -
Viral Vectors 101: Integrase-Deficient Lentiviral Vectors
TypeBlog PostPublishedMay 13, 2025, 1:15 p.m.... IDLVs were first discovered by researchers investigating HIV integrase mutants in the early 1990s (Leavitt...wish you the best of luck with any experiments involving IDLVs. And remember: You can never trust a lentivirus...of SCID-X1 patients. The Journal of Clinical Investigation, 118(9), 3143–3150. https://doi.org/10.1172/... -
Replacing Paper: Tips for Choosing an Electronic lab Notebook
TypeBlog PostPublishedJuly 10, 2018, 1:08 p.m....means to manage their work, combining data, team, inventory and project management functionalities with experiment...protocols. To facilitate this, some ELNs allow you to invite people and assign them roles and permission that... all data is organized within an ELN already. Investing some time in deploying a software solution that... -
Transferable Skills Guide: Conflict Resolution
TypeBlog PostPublishedJan. 4, 2022, 2:15 p.m.... of conflicts is important for the individuals involved, and also for the labs, organizations, or businesses... when you know that a person or team should be involved in making a decision and you see no record of ... means the other party will feel punished or invalidated. Both compromise and arbitration might remedy... -
Five Popular Model Organisms
TypeBlog PostPublishedApril 11, 2019, 12:39 p.m.... genes mutated in human cancers have a homolog involved in yeast cell division (Pray, 2008). Scientific...common protein found in lewy bodies), have been invaluable in understanding the basic molecular mechanisms...other model organisms. Worms have been used to investigate an array of biological systems and processes ... -
The Fluorescent Vegetables in Aptamer Soup
TypeBlog PostPublishedJan. 29, 2019, 2:27 p.m....2013). Since then, Spinach2 has been used to investigate intracellular viral DNA dynamics in neuronal ...developed into a split aptamer that can be used to investigate RNA assembly. The split aptamer is composed of... blog Learn more about aptamer fluorophores Investigate other fluorescent biosensors Find other uses ... -
FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution
TypeBlog PostPublishedOct. 17, 2019, 12:28 p.m....and optogenetics meet, allowing scientists to investigate the fundamental basis of both brain function ... flip-excision) switch (aka DIO (Double-floxed Inverse Orientation) or DO (Double-floxed Orientation))...recognition sites, its presence induces first opsin inversion, and then lox sites excision, therefore locking...