We narrowed to 111 results for: max
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TypeBlog PostPublishedMay 31, 2016, 3:30 p.m....Importantly, by varying these ratios, you can either maximize or minimize expression levels of your gene of ...
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Plasmids 101: Restriction Cloning
TypeBlog PostPublishedFeb. 18, 2016, 3:42 p.m....quantities of DNA, you’ll need to do a midiprep or a maxiprep. In these purifications, you generally lyse the... -
9 tips for a successful postdoctoral experience
TypeBlog PostPublishedOct. 9, 2018, 12:21 p.m....early on. Once your system is working, you should maximize data production with the end goal of having sufficient... -
Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)
TypeBlog PostPublishedMarch 7, 2017, 3:30 p.m....and are described in Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9... -
Plasmids 101: Using Transposons in the Lab
TypeBlog PostPublishedJuly 6, 2021, 1:28 p.m....mammalian cells without decreased efficiency, with a maximum cargo capacity of ~200 kB of DNA. Similar to piggyBac... -
Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
TypeBlog PostPublishedJune 28, 2018, 3:01 p.m....cells were transfected in 96-well format using an Amaxa Shuttle device; 5 days after the transfection, the... -
Special Delivery: Fluorophore Targeting for FRET Studies
TypeBlog PostPublishedJuly 19, 2016, 2:30 p.m....FRET acceptor. These experiments will define the maximum and minimum energy transfer values you can expect... -
A Needle in a Base-Stack: Cas9 Structural Biology
TypeBlog PostPublishedJune 4, 2024, 1:15 p.m....duplex, and it’s the reason guide sequences have a maximum length of 20 bases: nothing longer will fit. Stabilization... -
Antibody Validation for Flow Cytometry
TypeBlog PostPublishedSept. 10, 2024, 1:15 p.m.... further optimization can be performed to both maximize the separation and reduce any background staining... -
CRISPR/Cas9 FAQs Answered!
TypeBlog PostPublishedMarch 13, 2014, 4:08 p.m....tract lengths seem much shorter. Q10: What is the maximum amount of DNA that can be inserted into the genome... -
Troubleshooting and Optimizing a Western Blot
TypeBlog PostPublishedSept. 17, 2024, 1:15 p.m....that antibodies may need longer to reach their maximum binding, so feel free to trial even longer incubation...