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...; Hellemans J; Huggett J; Kubista M; Mueller R; Nolan T; Pfaffl MW; Shipley GL; Vandesompele J; Wittwer...abundances using SYBR Green I and real-time RT-PCR. J. Immunol. Methods 283:291–306.Panina, Y., Germond, A., Masui...Reproducible Data the First Time, Trends in Biotechnology, Volume 37, Issue 7, 2019, Pages 761-774, SSN... for low copy number target DNA. Journal of Immunological Methods 270 (2002) 119 – 133Tichopad A; Dilger...

... the United States, and many universities and Technology Transfer Offices were, at least, temporarily ...                           California Institute of Technology             Johns Hopkins University                           ...will initiate a conversation with you and your technology transfer office to discuss the possibilities ...

...2015 Find Hot Plasmid Technologies Write about Your Favorite Plasmid Technology Resources on Addgene....it is requested from Addgene many times as the technology disseminates throughout the research community...

...address a topic in a mechanistic way using the technology available.” Choosing a research project “entails...entails good judgement with the current technology to identify a problem, asking what approach will I take... importance of combining “new discoveries or technologies increase chances that your project will be fresh...

...excited about our new partnership with Allele Biotechnology which allows researchers to deposit plasmids...mTFP1 and mWasabi, as fluorophores from Allele Biotechnology that now can be deposited at Addgene. What makes...colleagues from The Scintillon Institute, Allele Biotechnology, Florida State University, Indiana University...

...many developments in science and the plasmids, technologies, and researchers behind them. As part of our...started using plasmids and the revolutionary CRISPR technology in their own labs. We’re overjoyed to provide...will be sure to keep our eyes open for any new technologies that will bring about the next revolution in...

... to Predict Vulnerable Patients. Frontiers in Immunology, 13. https://www.frontiersin.org/articles/10.3389...adverse reactions to drugs: adverse reaction terminology. WHO Collaborating Centre for International Drug....2022.952326Joubert, M. K., Deshpande, M., Yang, J., Reynolds, H., Bryson, C., Fogg, M., Baker, M. P., Herskovitz...

...They created an endogenous ApoB-NanoLuc reporter by inserting the NanoLuc luciferase downstream of the apoB...activation in bacteria Article contributed by Will Arnold Baojun Wang’s lab recently developed a CRISPR tool...

...tools by making their own fluorescent protein technologies widely available to the research community. ... a key advantage over other inducible enzyme technologies. She hopes that other researchers will be able... HMOs. Current HPLC-MS and fluorophore-based technologies are too time consuming in the first case and...

... via Single-Cell RNA Sequencing. Frontiers in immunology, 12, 730825. https://doi.org/10.3389/fimmu.2021.730825... Gradinaru V, Fox AS. (2023). Advances in AAV technology for delivering genetically encoded cargo to the...throughout the non-human primate brain. Nature Nanotechnology, 1–11. https://doi.org/10.1038/s41565-023-01419...widespread gene transfer to the adult brain. Nature Biotechnology, 34(2), 204–209. https://doi.org/10.1038/nbt...tropism of viral vectors in tissues. Nature Biotechnology. https://doi.org/10.1038/s41587-022-01648-w ...

...γ-retrovirus packaging cell line developed by Garry Nolan at Stanford (Swift, 2001) contains Gag-Pol and either...resources References Swift S, Lorens J, Achacoso P, Nolan GP. Rapid production of retroviruses for efficient...cells using 293T cell-based systems. Curr Protoc Immunol. 2001 May;Chapter 10:Unit 10.17C. doi: 10.1002/...

...acids in the domain). Yan et al. from Arbor Biotechnologies found that RspCas13d accessory protein RspWYL1...functional in multiple species (read Arbor Biotechnologies' press release about their Cas13d deposit here...Addgene. Learn more about research at Arbor Biotechnologies. Additional Resources on the Addgene Blog:...

... and BioID. In the Ting Lab’s recent Nature Biotechnology paper, they describe TurboID and miniTurbo, ... the TurboID plasmids here! Branon et al. Nat Biotechnol. 2018 PubMed PMID: 30125270. Changing the way...active LacZ tetramer.  This tetramer cleaves chlorophenol red-b-D-galactopyranoside on the assay paper...

...CRISPR 101" post. Next-generation sequencing technologies are the gold standard but they remain cost-prohibitive...introduce digital PCR as an emerging validation technology. Digital PCR has several advantages over mismatch...CRISPR-Cas nucleases in human cells. Nature Biotechnology 31, 822–826 (2013). PubMed PMID: 23792628. PubMed...

...to essentially any DNA sequence desired. This technology allows you to create a pair of designer proteins...on traditional homologous recombination. That technology had been used for decades to generate knock-out...That’s the revolutionary aspect: TALENs take a technology that was difficult for all but the most experienced...

...Gibson Assembly Cloning and Gateway® cloning technologies, as well as several others. Each method is unique...biologists have harnessed it to evolve cloning technology. CcdB: A potent toxin... The ccdB gene, located...thoroughly screen colonies for the insert. Gateway® technology (developed by InvitrogenTM) is essentially a ...

...to your cloning efforts. Golden Gate cloning technology relies on Type IIS restriction enzymes, first...Voytas groups described a new Golden Gate-based technology for genome editing which allowed for the ordered...efficiently in just a few steps. More recently, CRISPR technology has adapted Golden Gate cloning for inserting...

... the limitations of current state of the art technologies. Therefore, take time to review the literature...insight into what the desirable features of future technologies should offer in order to build upon and address...proposal to test hypotheses and develop novel technologies! If data from a publication or preliminary data...

...targeting of long non-coding RNA genes. Nature biotechnology. 2020;38(5):573. doi:10.1038/s41587-020-0428... cells by Cas9 targeting of splice sites. Nat Biotechnol. Published online November 5, 2018. doi:10.1038...Non-Coding RNAs in Plants with the CRISPR-Cas Technology is a Challenge yet Worth Accepting. Front Plant...

...There can be no doubt that CRISPR/Cas9 technology has been a breakthrough for the genome-editing field... obstacles for future CRISPR therapies CRISPR technology is rapidly evolving, and many of the challenges...taken advantage of what our current delivery technologies are suited for. For example, lipid nanoparticle...